Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4764 and GFF4765 are separated by 140 nucleotides GFF4765 and GFF4766 are separated by 672 nucleotides
GFF4764 - Type III secretion outermembrane pore forming protein (YscC,MxiD,HrcC, InvG), at 71,380 to 72,924
GFF4764
GFF4765 - Type III secretion thermoregulatory protein (LcrF,VirF,transcription regulation of virulence plasmid), at 73,065 to 73,814
GFF4765
GFF4766 - Invasion protein invH precursor, at 74,487 to 74,615
GFF4766
Position (kb)
73
74 Strain fitness (log2 ratio)
-1
0
1 at 72.078 kb on + strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.083 kb on - strand, within GFF4764 at 72.270 kb on + strand, within GFF4764 at 72.271 kb on - strand, within GFF4764 at 72.399 kb on + strand, within GFF4764 at 72.400 kb on - strand, within GFF4764 at 72.459 kb on + strand, within GFF4764 at 72.507 kb on + strand, within GFF4764 at 72.598 kb on - strand, within GFF4764 at 72.627 kb on - strand, within GFF4764 at 72.676 kb on + strand, within GFF4764 at 72.765 kb on - strand, within GFF4764 at 72.985 kb on + strand at 73.011 kb on + strand at 73.301 kb on - strand, within GFF4765 at 73.362 kb on + strand, within GFF4765 at 73.410 kb on + strand, within GFF4765 at 73.416 kb on - strand, within GFF4765 at 73.534 kb on + strand, within GFF4765 at 73.600 kb on - strand, within GFF4765 at 73.715 kb on + strand, within GFF4765 at 73.716 kb on - strand, within GFF4765 at 74.301 kb on - strand at 74.569 kb on + strand, within GFF4766 at 74.612 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 72,078 + GFF4764 0.45 -0.4 72,083 - GFF4764 0.46 +0.1 72,083 - GFF4764 0.46 +0.3 72,270 + GFF4764 0.58 +0.3 72,271 - GFF4764 0.58 +0.1 72,399 + GFF4764 0.66 +0.4 72,400 - GFF4764 0.66 +0.7 72,459 + GFF4764 0.70 -0.2 72,507 + GFF4764 0.73 +0.2 72,598 - GFF4764 0.79 +0.3 72,627 - GFF4764 0.81 +0.1 72,676 + GFF4764 0.84 +0.1 72,765 - GFF4764 0.90 -0.0 72,985 + +0.1 73,011 + -0.6 73,301 - GFF4765 0.31 +0.3 73,362 + GFF4765 0.40 -0.4 73,410 + GFF4765 0.46 -0.6 73,416 - GFF4765 0.47 -0.3 73,534 + GFF4765 0.63 -1.1 73,600 - GFF4765 0.71 -0.3 73,715 + GFF4765 0.87 -1.3 73,716 - GFF4765 0.87 -1.4 74,301 - +0.3 74,569 + GFF4766 0.64 +0.1 74,612 - +0.5
Or see this region's nucleotide sequence