Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4733 and GFF4734 are separated by 344 nucleotides GFF4734 and GFF4735 are separated by 86 nucleotides GFF4735 and GFF4736 overlap by 44 nucleotides GFF4736 and GFF4737 overlap by 8 nucleotides
GFF4733 - Type III secretion transcriptional regulator HilC (= SirC), at 41,890 to 42,777
GFF4733
GFF4734 - Putative effector protein OrgC of SPI-1 type III secretion system, at 43,122 to 43,484
GFF4734
GFF4735 - OrgB protein, associated with InvC ATPase of type III secretion system, at 43,571 to 44,251
GFF4735
GFF4736 - Oxygen-regulated invasion protein OrgA, at 44,208 to 44,786
GFF4736
GFF4737 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 44,779 to 45,537
GFF4737
Position (kb) 43
44
45 Strain fitness (log2 ratio) -1
0
1 at 43.026 kb on + strand at 43.070 kb on + strand at 43.081 kb on - strand at 43.082 kb on + strand at 43.085 kb on - strand at 43.085 kb on - strand at 43.097 kb on - strand at 43.365 kb on - strand, within GFF4734 at 43.476 kb on - strand at 43.546 kb on + strand at 43.547 kb on - strand at 43.616 kb on - strand at 43.682 kb on - strand, within GFF4735 at 43.754 kb on - strand, within GFF4735 at 43.778 kb on - strand, within GFF4735 at 43.784 kb on - strand, within GFF4735 at 44.014 kb on + strand, within GFF4735 at 44.122 kb on + strand, within GFF4735 at 44.188 kb on + strand at 44.251 kb on + strand at 44.252 kb on - strand at 44.426 kb on + strand, within GFF4736 at 44.440 kb on - strand, within GFF4736 at 44.548 kb on + strand, within GFF4736 at 44.649 kb on + strand, within GFF4736 at 44.728 kb on + strand, within GFF4736 at 44.729 kb on - strand at 44.729 kb on - strand at 44.781 kb on - strand at 44.850 kb on - strand at 45.000 kb on - strand, within GFF4737 at 45.078 kb on - strand, within GFF4737 at 45.079 kb on + strand, within GFF4737 at 45.080 kb on - strand, within GFF4737 at 45.144 kb on - strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.148 kb on - strand, within GFF4737 at 45.223 kb on + strand, within GFF4737
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 43,026 + -0.3 43,070 + -0.4 43,081 - +0.2 43,082 + +0.1 43,085 - -0.1 43,085 - +0.1 43,097 - -0.2 43,365 - GFF4734 0.67 -0.3 43,476 - +0.2 43,546 + -0.9 43,547 - -0.6 43,616 - -0.6 43,682 - GFF4735 0.16 -0.7 43,754 - GFF4735 0.27 -0.1 43,778 - GFF4735 0.30 +0.3 43,784 - GFF4735 0.31 -0.3 44,014 + GFF4735 0.65 -0.6 44,122 + GFF4735 0.81 -0.1 44,188 + -0.3 44,251 + -0.4 44,252 - -0.0 44,426 + GFF4736 0.38 +0.2 44,440 - GFF4736 0.40 -0.6 44,548 + GFF4736 0.59 +0.2 44,649 + GFF4736 0.76 +0.9 44,728 + GFF4736 0.90 +0.1 44,729 - +0.2 44,729 - +0.1 44,781 - +0.3 44,850 - -0.0 45,000 - GFF4737 0.29 -0.1 45,078 - GFF4737 0.39 -0.0 45,079 + GFF4737 0.40 -0.1 45,080 - GFF4737 0.40 +0.1 45,144 - GFF4737 0.48 -0.7 45,147 + GFF4737 0.48 -0.0 45,147 + GFF4737 0.48 +0.8 45,148 - GFF4737 0.49 -0.3 45,223 + GFF4737 0.58 +0.7
Or see this region's nucleotide sequence