Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4721 and GFF4722 overlap by 10 nucleotides GFF4722 and GFF4723 overlap by 1 nucleotides GFF4723 and GFF4724 overlap by 4 nucleotides
GFF4721 - [NiFe] hydrogenase nickel incorporation-associated protein HypB, at 30,003 to 30,875
GFF4721
GFF4722 - [NiFe] hydrogenase metallocenter assembly protein HypC, at 30,866 to 31,138
GFF4722
GFF4723 - [NiFe] hydrogenase metallocenter assembly protein HypD, at 31,138 to 32,259
GFF4723
GFF4724 - [NiFe] hydrogenase metallocenter assembly protein HypE, at 32,256 to 33,266
GFF4724
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-1
0
1 at 30.266 kb on + strand, within GFF4721 at 30.398 kb on - strand, within GFF4721 at 30.627 kb on - strand, within GFF4721 at 31.084 kb on + strand, within GFF4722 at 31.136 kb on + strand at 31.137 kb on - strand at 31.157 kb on - strand at 31.305 kb on - strand, within GFF4723 at 31.497 kb on + strand, within GFF4723 at 31.691 kb on + strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.833 kb on - strand, within GFF4723 at 31.897 kb on + strand, within GFF4723 at 32.083 kb on + strand, within GFF4723 at 32.084 kb on - strand, within GFF4723 at 32.084 kb on - strand, within GFF4723 at 32.266 kb on + strand at 32.267 kb on - strand at 32.267 kb on - strand at 32.324 kb on + strand at 32.433 kb on + strand, within GFF4724 at 32.433 kb on + strand, within GFF4724 at 32.434 kb on - strand, within GFF4724 at 32.597 kb on + strand, within GFF4724 at 32.683 kb on + strand, within GFF4724 at 32.688 kb on - strand, within GFF4724 at 32.777 kb on + strand, within GFF4724 at 32.864 kb on - strand, within GFF4724 at 32.892 kb on - strand, within GFF4724 at 33.207 kb on + strand at 33.250 kb on + strand at 33.257 kb on + strand at 33.259 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 30,266 + GFF4721 0.30 +0.2 30,398 - GFF4721 0.45 +0.1 30,627 - GFF4721 0.71 +0.6 31,084 + GFF4722 0.80 +0.2 31,136 + -0.1 31,137 - -0.7 31,157 - +0.7 31,305 - GFF4723 0.15 -0.5 31,497 + GFF4723 0.32 -0.1 31,691 + GFF4723 0.49 -0.3 31,833 - GFF4723 0.62 +0.1 31,833 - GFF4723 0.62 -0.2 31,897 + GFF4723 0.68 -0.1 32,083 + GFF4723 0.84 +0.0 32,084 - GFF4723 0.84 -0.2 32,084 - GFF4723 0.84 -0.4 32,266 + +1.1 32,267 - -0.5 32,267 - -0.1 32,324 + -0.0 32,433 + GFF4724 0.18 +0.1 32,433 + GFF4724 0.18 +0.0 32,434 - GFF4724 0.18 -0.1 32,597 + GFF4724 0.34 -0.2 32,683 + GFF4724 0.42 +0.7 32,688 - GFF4724 0.43 +0.1 32,777 + GFF4724 0.52 +0.3 32,864 - GFF4724 0.60 +0.2 32,892 - GFF4724 0.63 +0.3 33,207 + -0.0 33,250 + -1.0 33,257 + +0.3 33,259 + -0.2
Or see this region's nucleotide sequence