Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4647 and GFF4648 are separated by 14 nucleotides GFF4648 and GFF4649 are separated by 136 nucleotides GFF4649 and GFF4650 are separated by 291 nucleotides GFF4650 and GFF4651 overlap by 4 nucleotides
GFF4647 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 441,657 to 442,796
GFF4647
GFF4648 - Cyd operon protein YbgT, at 442,811 to 442,924
GFF4648
GFF4649 - Cyd operon protein YbgE, at 443,061 to 443,342
GFF4649
GFF4650 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 443,634 to 443,975
GFF4650
GFF4651 - MotA/TolQ/ExbB proton channel family protein, at 443,972 to 444,664
GFF4651
Position (kb) 443
444 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 442.090 kb on + strand, within GFF4647 at 442.215 kb on + strand, within GFF4647 at 442.292 kb on - strand, within GFF4647 at 442.326 kb on + strand, within GFF4647 at 442.326 kb on + strand, within GFF4647 at 442.327 kb on - strand, within GFF4647 at 442.332 kb on - strand, within GFF4647 at 442.494 kb on - strand, within GFF4647 at 442.563 kb on - strand, within GFF4647 at 442.792 kb on - strand at 442.795 kb on - strand at 442.798 kb on + strand at 442.922 kb on + strand at 442.922 kb on + strand at 442.933 kb on - strand at 442.941 kb on + strand at 442.950 kb on + strand at 443.004 kb on + strand at 443.004 kb on + strand at 443.004 kb on + strand at 443.004 kb on + strand at 443.012 kb on + strand at 443.056 kb on - strand at 443.056 kb on - strand at 443.068 kb on - strand at 443.074 kb on - strand at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.141 kb on + strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.142 kb on - strand, within GFF4649 at 443.204 kb on - strand, within GFF4649 at 443.441 kb on + strand at 443.444 kb on + strand at 443.445 kb on - strand at 443.445 kb on - strand at 443.445 kb on - strand at 443.463 kb on + strand at 443.487 kb on + strand at 443.487 kb on + strand at 443.496 kb on + strand at 443.508 kb on + strand at 443.576 kb on + strand at 443.580 kb on + strand at 443.581 kb on - strand at 443.652 kb on + strand at 443.769 kb on + strand, within GFF4650 at 443.793 kb on - strand, within GFF4650 at 443.881 kb on + strand, within GFF4650 at 443.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 442,090 + GFF4647 0.38 -1.6 442,215 + GFF4647 0.49 -1.8 442,292 - GFF4647 0.56 -2.7 442,326 + GFF4647 0.59 -2.9 442,326 + GFF4647 0.59 -1.5 442,327 - GFF4647 0.59 -2.5 442,332 - GFF4647 0.59 -2.1 442,494 - GFF4647 0.73 -1.8 442,563 - GFF4647 0.79 -1.7 442,792 - -1.9 442,795 - -1.4 442,798 + -0.3 442,922 + +0.5 442,922 + +0.1 442,933 - -0.3 442,941 + +0.3 442,950 + +0.3 443,004 + +0.2 443,004 + -0.1 443,004 + +0.4 443,004 + -0.2 443,012 + +0.3 443,056 - -0.3 443,056 - -0.6 443,068 - -0.5 443,074 - +0.2 443,141 + GFF4649 0.28 +0.3 443,141 + GFF4649 0.28 -0.4 443,141 + GFF4649 0.28 -0.2 443,141 + GFF4649 0.28 -0.3 443,142 - GFF4649 0.29 -0.7 443,142 - GFF4649 0.29 -0.0 443,142 - GFF4649 0.29 +0.2 443,142 - GFF4649 0.29 +0.6 443,204 - GFF4649 0.51 +0.6 443,441 + +0.2 443,444 + -0.5 443,445 - -0.2 443,445 - -0.4 443,445 - -0.3 443,463 + +0.5 443,487 + +0.4 443,487 + +0.5 443,496 + -0.8 443,508 + +0.2 443,576 + -1.0 443,580 + -0.3 443,581 - -0.5 443,652 + +0.5 443,769 + GFF4650 0.39 -0.2 443,793 - GFF4650 0.46 +0.2 443,881 + GFF4650 0.72 -0.8 443,945 + +1.1
Or see this region's nucleotide sequence