Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4156 and GFF4157 are separated by 315 nucleotides GFF4157 and GFF4158 are separated by 145 nucleotides GFF4158 and GFF4159 are separated by 236 nucleotides GFF4159 and GFF4160 are separated by 4 nucleotides
GFF4156 - T1SS secreted agglutinin RTX, at 38,443 to 49,917
GFF4156
GFF4157 - hypothetical protein, at 50,233 to 50,385
GFF4157
GFF4158 - tmRNA-binding protein SmpB, at 50,531 to 51,013
GFF4158
GFF4159 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 51,250 to 51,639
GFF4159
GFF4160 - UPF0125 protein yfjF, at 51,644 to 51,919
GFF4160
Position (kb)
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 49.612 kb on - strand at 49.667 kb on - strand at 49.693 kb on + strand at 49.918 kb on - strand at 49.918 kb on - strand at 50.123 kb on - strand at 50.424 kb on + strand at 50.458 kb on + strand at 50.851 kb on - strand, within GFF4158 at 50.943 kb on + strand, within GFF4158 at 51.035 kb on - strand at 51.085 kb on - strand at 51.089 kb on + strand at 51.090 kb on - strand at 51.090 kb on - strand at 51.119 kb on - strand at 51.161 kb on - strand at 51.171 kb on - strand at 51.260 kb on + strand at 51.680 kb on + strand, within GFF4160 at 51.781 kb on + strand, within GFF4160 at 51.786 kb on + strand, within GFF4160 at 51.787 kb on - strand, within GFF4160 at 51.794 kb on + strand, within GFF4160 at 51.882 kb on + strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.883 kb on - strand, within GFF4160 at 51.913 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 49,612 - -2.5 49,667 - -0.2 49,693 + -0.0 49,918 - +0.2 49,918 - -0.6 50,123 - -1.3 50,424 + -0.2 50,458 + +1.0 50,851 - GFF4158 0.66 -0.6 50,943 + GFF4158 0.85 -0.5 51,035 - +0.4 51,085 - +0.1 51,089 + +0.3 51,090 - +0.1 51,090 - +0.1 51,119 - +0.2 51,161 - +0.5 51,171 - -0.8 51,260 + -3.3 51,680 + GFF4160 0.13 -1.2 51,781 + GFF4160 0.50 -1.0 51,786 + GFF4160 0.51 -0.3 51,787 - GFF4160 0.52 -0.8 51,794 + GFF4160 0.54 +0.7 51,882 + GFF4160 0.86 -1.7 51,883 - GFF4160 0.87 +0.0 51,883 - GFF4160 0.87 -0.3 51,913 + -0.8
Or see this region's nucleotide sequence