Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4149 and GFF4150 are separated by 443 nucleotides GFF4150 and GFF4151 are separated by 1 nucleotides GFF4151 and GFF4152 are separated by 13 nucleotides
GFF4149 - putative cytoplasmic protein, at 29,552 to 30,691
GFF4149
GFF4150 - putative periplasmic protein, at 31,135 to 31,773
GFF4150
GFF4151 - integrase-like protein, at 31,775 to 31,942
GFF4151
GFF4152 - Phage integrase, Phage P4-associated, at 31,956 to 33,020
GFF4152
Position (kb)
31
32 Strain fitness (log2 ratio)
-1
0
1 at 30.414 kb on - strand, within GFF4149 at 30.460 kb on + strand, within GFF4149 at 30.532 kb on - strand, within GFF4149 at 30.532 kb on - strand, within GFF4149 at 30.632 kb on - strand at 30.632 kb on - strand at 30.740 kb on + strand at 30.771 kb on - strand at 30.785 kb on - strand at 30.952 kb on - strand at 30.952 kb on - strand at 30.990 kb on + strand at 30.991 kb on - strand at 31.043 kb on + strand at 31.043 kb on + strand at 31.072 kb on - strand at 31.238 kb on - strand, within GFF4150 at 31.238 kb on - strand, within GFF4150 at 31.477 kb on - strand, within GFF4150 at 31.515 kb on + strand, within GFF4150 at 31.550 kb on + strand, within GFF4150 at 31.627 kb on - strand, within GFF4150 at 31.631 kb on + strand, within GFF4150 at 31.729 kb on - strand at 32.069 kb on + strand, within GFF4152 at 32.069 kb on + strand, within GFF4152 at 32.069 kb on + strand, within GFF4152 at 32.287 kb on - strand, within GFF4152 at 32.481 kb on - strand, within GFF4152 at 32.736 kb on + strand, within GFF4152
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 30,414 - GFF4149 0.76 +0.4 30,460 + GFF4149 0.80 -1.1 30,532 - GFF4149 0.86 +0.1 30,532 - GFF4149 0.86 +0.2 30,632 - +0.0 30,632 - +0.3 30,740 + +0.6 30,771 - -0.3 30,785 - +0.1 30,952 - +0.3 30,952 - -0.2 30,990 + -0.4 30,991 - +0.5 31,043 + +0.6 31,043 + +0.1 31,072 - +0.9 31,238 - GFF4150 0.16 +0.5 31,238 - GFF4150 0.16 +0.3 31,477 - GFF4150 0.54 -0.2 31,515 + GFF4150 0.59 +0.5 31,550 + GFF4150 0.65 +0.7 31,627 - GFF4150 0.77 +0.0 31,631 + GFF4150 0.78 +0.5 31,729 - -1.1 32,069 + GFF4152 0.11 +0.8 32,069 + GFF4152 0.11 -0.5 32,069 + GFF4152 0.11 +0.0 32,287 - GFF4152 0.31 +0.1 32,481 - GFF4152 0.49 +0.8 32,736 + GFF4152 0.73 -0.1
Or see this region's nucleotide sequence