Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4028 and GFF4029 are separated by 256 nucleotides GFF4029 and GFF4030 are separated by 78 nucleotides
GFF4028 - Flagellar hook-associated protein FliD, at 294,048 to 295,451
GFF4028
GFF4029 - Flagellar biosynthesis protein FliC, at 295,708 to 297,195
GFF4029
GFF4030 - Lysine-N-methylase (EC 2.1.1.-), at 297,274 to 298,479
GFF4030
Position (kb) 295
296
297
298 Strain fitness (log2 ratio) -1
0
1 at 295.059 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.167 kb on - strand, within GFF4028 at 295.169 kb on - strand, within GFF4028 at 295.645 kb on + strand at 295.688 kb on + strand at 295.722 kb on + strand at 296.395 kb on + strand, within GFF4029 at 296.508 kb on + strand, within GFF4029 at 296.631 kb on + strand, within GFF4029 at 296.641 kb on + strand, within GFF4029 at 296.642 kb on - strand, within GFF4029 at 296.704 kb on + strand, within GFF4029 at 296.739 kb on + strand, within GFF4029 at 296.742 kb on + strand, within GFF4029 at 296.913 kb on + strand, within GFF4029 at 297.018 kb on + strand, within GFF4029 at 297.018 kb on + strand, within GFF4029 at 297.038 kb on + strand, within GFF4029 at 297.185 kb on + strand at 297.223 kb on + strand at 297.609 kb on + strand, within GFF4030 at 297.631 kb on - strand, within GFF4030 at 297.631 kb on - strand, within GFF4030 at 297.826 kb on + strand, within GFF4030 at 297.850 kb on + strand, within GFF4030 at 297.850 kb on + strand, within GFF4030 at 297.850 kb on + strand, within GFF4030 at 297.851 kb on - strand, within GFF4030 at 297.907 kb on + strand, within GFF4030 at 298.050 kb on + strand, within GFF4030
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 295,059 - GFF4028 0.72 +0.1 295,167 - GFF4028 0.80 -0.5 295,167 - GFF4028 0.80 +0.6 295,167 - GFF4028 0.80 +0.0 295,167 - GFF4028 0.80 -0.3 295,169 - GFF4028 0.80 +0.0 295,645 + +0.9 295,688 + +1.0 295,722 + +1.3 296,395 + GFF4029 0.46 -0.3 296,508 + GFF4029 0.54 +1.5 296,631 + GFF4029 0.62 +1.0 296,641 + GFF4029 0.63 +1.2 296,642 - GFF4029 0.63 -0.8 296,704 + GFF4029 0.67 +1.5 296,739 + GFF4029 0.69 +0.7 296,742 + GFF4029 0.69 +0.3 296,913 + GFF4029 0.81 +1.7 297,018 + GFF4029 0.88 +0.7 297,018 + GFF4029 0.88 +0.7 297,038 + GFF4029 0.89 +0.7 297,185 + +0.8 297,223 + +0.7 297,609 + GFF4030 0.28 +0.1 297,631 - GFF4030 0.30 -0.8 297,631 - GFF4030 0.30 -0.2 297,826 + GFF4030 0.46 -0.2 297,850 + GFF4030 0.48 +0.9 297,850 + GFF4030 0.48 +0.5 297,850 + GFF4030 0.48 +0.1 297,851 - GFF4030 0.48 -0.2 297,907 + GFF4030 0.52 +0.4 298,050 + GFF4030 0.64 +0.7
Or see this region's nucleotide sequence