Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3996 and GFF3997 overlap by 20 nucleotides GFF3997 and GFF3998 overlap by 12 nucleotides GFF3998 and GFF3999 are separated by 175 nucleotides
GFF3996 - DNA-cytosine methyltransferase (EC 2.1.1.37), at 269,094 to 270,524
GFF3996
GFF3997 - Very-short-patch mismatch repair endonuclease (G-T specific), at 270,505 to 270,975
GFF3997
GFF3998 - Permease of the drug/metabolite transporter (DMT) superfamily, at 270,964 to 271,884
GFF3998
GFF3999 - Putative inner membrane protein, at 272,060 to 272,971
GFF3999
Position (kb)
270
271
272 Strain fitness (log2 ratio)
-1
0
1 at 270.069 kb on + strand, within GFF3996 at 270.101 kb on - strand, within GFF3996 at 270.148 kb on - strand, within GFF3996 at 270.159 kb on + strand, within GFF3996 at 270.205 kb on - strand, within GFF3996 at 270.673 kb on + strand, within GFF3997 at 270.724 kb on + strand, within GFF3997 at 270.725 kb on - strand, within GFF3997 at 270.725 kb on - strand, within GFF3997 at 270.805 kb on - strand, within GFF3997 at 270.965 kb on + strand at 271.194 kb on + strand, within GFF3998 at 271.194 kb on + strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.195 kb on - strand, within GFF3998 at 271.216 kb on - strand, within GFF3998 at 271.414 kb on + strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.451 kb on - strand, within GFF3998 at 271.545 kb on + strand, within GFF3998 at 271.557 kb on + strand, within GFF3998 at 271.557 kb on + strand, within GFF3998 at 271.658 kb on - strand, within GFF3998 at 271.658 kb on - strand, within GFF3998 at 271.835 kb on + strand at 271.835 kb on + strand at 271.865 kb on - strand at 271.868 kb on - strand at 272.022 kb on + strand at 272.022 kb on + strand at 272.022 kb on + strand at 272.026 kb on + strand at 272.027 kb on - strand at 272.027 kb on - strand at 272.085 kb on + strand at 272.116 kb on + strand at 272.256 kb on + strand, within GFF3999 at 272.383 kb on + strand, within GFF3999 at 272.384 kb on - strand, within GFF3999 at 272.384 kb on - strand, within GFF3999 at 272.484 kb on + strand, within GFF3999 at 272.621 kb on + strand, within GFF3999 at 272.635 kb on + strand, within GFF3999 at 272.639 kb on - strand, within GFF3999 at 272.817 kb on + strand, within GFF3999
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 270,069 + GFF3996 0.68 -0.2 270,101 - GFF3996 0.70 +0.3 270,148 - GFF3996 0.74 -0.3 270,159 + GFF3996 0.74 -0.1 270,205 - GFF3996 0.78 -0.5 270,673 + GFF3997 0.36 -0.1 270,724 + GFF3997 0.46 +0.5 270,725 - GFF3997 0.47 +1.0 270,725 - GFF3997 0.47 +0.1 270,805 - GFF3997 0.64 -0.9 270,965 + +1.1 271,194 + GFF3998 0.25 -0.3 271,194 + GFF3998 0.25 +0.4 271,195 - GFF3998 0.25 +0.1 271,195 - GFF3998 0.25 -0.1 271,195 - GFF3998 0.25 +0.5 271,216 - GFF3998 0.27 +0.8 271,414 + GFF3998 0.49 +0.6 271,451 - GFF3998 0.53 +0.7 271,451 - GFF3998 0.53 +1.3 271,451 - GFF3998 0.53 +0.1 271,545 + GFF3998 0.63 -0.8 271,557 + GFF3998 0.64 +1.2 271,557 + GFF3998 0.64 +0.4 271,658 - GFF3998 0.75 -0.3 271,658 - GFF3998 0.75 +0.6 271,835 + +0.9 271,835 + -0.0 271,865 - +0.5 271,868 - +0.6 272,022 + -0.0 272,022 + +0.0 272,022 + +0.1 272,026 + -0.2 272,027 - -0.4 272,027 - +1.2 272,085 + +0.2 272,116 + +0.2 272,256 + GFF3999 0.21 +0.4 272,383 + GFF3999 0.35 +0.1 272,384 - GFF3999 0.36 +0.4 272,384 - GFF3999 0.36 +0.3 272,484 + GFF3999 0.46 +0.5 272,621 + GFF3999 0.62 -0.5 272,635 + GFF3999 0.63 +0.9 272,639 - GFF3999 0.63 -0.6 272,817 + GFF3999 0.83 +0.9
Or see this region's nucleotide sequence