Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF393 and GFF394 are separated by 9 nucleotides GFF394 and GFF395 are separated by 493 nucleotides
GFF393 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 107,965 to 109,161
GFF393
GFF394 - L-threonine 3-dehydrogenase (EC 1.1.1.103), at 109,171 to 110,196
GFF394
GFF395 - Beta-1,3-galactosyltransferase / Beta-1,4-galactosyltransferase, at 110,690 to 111,724
GFF395
Position (kb)
109
110
111 Strain fitness (log2 ratio)
-1
0
1
2 at 108.286 kb on + strand, within GFF393 at 108.349 kb on - strand, within GFF393 at 108.378 kb on + strand, within GFF393 at 108.378 kb on - strand, within GFF393 at 108.379 kb on - strand, within GFF393 at 108.422 kb on - strand, within GFF393 at 108.668 kb on + strand, within GFF393 at 108.668 kb on + strand, within GFF393 at 108.669 kb on - strand, within GFF393 at 108.672 kb on + strand, within GFF393 at 108.673 kb on - strand, within GFF393 at 108.673 kb on - strand, within GFF393 at 108.676 kb on - strand, within GFF393 at 108.776 kb on - strand, within GFF393 at 108.822 kb on + strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.016 kb on - strand, within GFF393 at 109.033 kb on + strand, within GFF393 at 109.104 kb on + strand at 109.167 kb on + strand at 109.168 kb on - strand at 109.168 kb on - strand at 109.261 kb on - strand at 109.303 kb on + strand, within GFF394 at 109.306 kb on - strand, within GFF394 at 109.363 kb on + strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.364 kb on - strand, within GFF394 at 109.540 kb on + strand, within GFF394 at 109.599 kb on - strand, within GFF394 at 109.670 kb on + strand, within GFF394 at 109.974 kb on + strand, within GFF394 at 110.198 kb on - strand at 110.202 kb on - strand at 110.202 kb on - strand at 110.202 kb on - strand at 110.416 kb on - strand at 110.803 kb on + strand, within GFF395 at 110.806 kb on + strand, within GFF395 at 110.996 kb on + strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 110.997 kb on - strand, within GFF395 at 111.013 kb on + strand, within GFF395
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 108,286 + GFF393 0.27 +0.3 108,349 - GFF393 0.32 +0.0 108,378 + GFF393 0.35 +0.4 108,378 - GFF393 0.35 +1.3 108,379 - GFF393 0.35 +0.1 108,422 - GFF393 0.38 +0.4 108,668 + GFF393 0.59 +0.1 108,668 + GFF393 0.59 +0.2 108,669 - GFF393 0.59 -0.6 108,672 + GFF393 0.59 -0.5 108,673 - GFF393 0.59 -0.4 108,673 - GFF393 0.59 -0.5 108,676 - GFF393 0.59 -0.6 108,776 - GFF393 0.68 +0.1 108,822 + GFF393 0.72 +0.0 109,016 - GFF393 0.88 -0.3 109,016 - GFF393 0.88 -0.2 109,016 - GFF393 0.88 -0.7 109,033 + GFF393 0.89 -0.3 109,104 + -0.1 109,167 + -0.5 109,168 - +0.0 109,168 - +0.0 109,261 - -0.7 109,303 + GFF394 0.13 +0.7 109,306 - GFF394 0.13 -1.2 109,363 + GFF394 0.19 -0.3 109,364 - GFF394 0.19 -0.9 109,364 - GFF394 0.19 -0.9 109,364 - GFF394 0.19 -0.9 109,364 - GFF394 0.19 -0.9 109,540 + GFF394 0.36 -0.3 109,599 - GFF394 0.42 -1.0 109,670 + GFF394 0.49 +0.1 109,974 + GFF394 0.78 -0.3 110,198 - -0.2 110,202 - +0.0 110,202 - -0.1 110,202 - -0.6 110,416 - -0.1 110,803 + GFF395 0.11 +0.5 110,806 + GFF395 0.11 +0.1 110,996 + GFF395 0.30 +0.7 110,997 - GFF395 0.30 +0.3 110,997 - GFF395 0.30 -0.3 110,997 - GFF395 0.30 -0.1 111,013 + GFF395 0.31 +1.9
Or see this region's nucleotide sequence