Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3588 and GFF3589 are separated by 35 nucleotides GFF3589 and GFF3590 are separated by 2 nucleotides GFF3590 and GFF3591 are separated by 99 nucleotides GFF3591 and GFF3592 are separated by 2 nucleotides
GFF3588 - Secretion system effector SseB, at 345,987 to 346,577
GFF3588
GFF3589 - Secretion system chaparone SscA, at 346,613 to 347,047
GFF3589
GFF3590 - Secretion system effector SseC, at 347,050 to 348,504
GFF3590
GFF3591 - Secretion system effector SseD, at 348,604 to 349,107
GFF3591
GFF3592 - Secretion system effector SseE, at 349,110 to 349,526
GFF3592
Position (kb)
347
348
349 Strain fitness (log2 ratio)
-2
-1
0
1 at 346.265 kb on + strand, within GFF3588 at 346.466 kb on - strand, within GFF3588 at 346.646 kb on - strand at 346.689 kb on - strand, within GFF3589 at 346.762 kb on - strand, within GFF3589 at 346.763 kb on + strand, within GFF3589 at 346.764 kb on - strand, within GFF3589 at 346.764 kb on - strand, within GFF3589 at 346.967 kb on - strand, within GFF3589 at 347.045 kb on + strand at 347.114 kb on + strand at 347.370 kb on + strand, within GFF3590 at 347.430 kb on + strand, within GFF3590 at 347.430 kb on + strand, within GFF3590 at 347.441 kb on + strand, within GFF3590 at 347.541 kb on - strand, within GFF3590 at 347.623 kb on + strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.712 kb on + strand, within GFF3590 at 347.727 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 348.436 kb on - strand at 348.443 kb on + strand at 348.444 kb on - strand at 348.446 kb on + strand at 348.447 kb on - strand at 348.836 kb on + strand, within GFF3591 at 348.948 kb on + strand, within GFF3591 at 349.168 kb on - strand, within GFF3592 at 349.186 kb on + strand, within GFF3592 at 349.190 kb on - strand, within GFF3592 at 349.230 kb on + strand, within GFF3592 at 349.316 kb on - strand, within GFF3592 at 349.336 kb on + strand, within GFF3592
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 346,265 + GFF3588 0.47 -0.4 346,466 - GFF3588 0.81 -0.1 346,646 - -2.5 346,689 - GFF3589 0.17 -0.0 346,762 - GFF3589 0.34 -0.2 346,763 + GFF3589 0.34 -0.3 346,764 - GFF3589 0.35 -0.9 346,764 - GFF3589 0.35 -0.1 346,967 - GFF3589 0.81 -0.1 347,045 + -0.9 347,114 + -0.2 347,370 + GFF3590 0.22 -0.4 347,430 + GFF3590 0.26 -0.0 347,430 + GFF3590 0.26 -0.7 347,441 + GFF3590 0.27 +0.2 347,541 - GFF3590 0.34 -0.5 347,623 + GFF3590 0.39 -0.4 347,624 - GFF3590 0.39 -0.3 347,624 - GFF3590 0.39 -0.5 347,624 - GFF3590 0.39 -0.6 347,712 + GFF3590 0.45 +0.8 347,727 + GFF3590 0.47 +0.2 347,868 + GFF3590 0.56 +0.2 347,868 + GFF3590 0.56 +0.1 348,436 - -0.3 348,443 + +0.4 348,444 - +0.0 348,446 + +0.8 348,447 - -0.6 348,836 + GFF3591 0.46 -0.1 348,948 + GFF3591 0.68 +0.2 349,168 - GFF3592 0.14 -0.3 349,186 + GFF3592 0.18 -0.1 349,190 - GFF3592 0.19 +0.0 349,230 + GFF3592 0.29 -0.2 349,316 - GFF3592 0.49 -0.2 349,336 + GFF3592 0.54 -0.2
Or see this region's nucleotide sequence