Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3453 and GFF3454 are separated by 196 nucleotides GFF3454 and GFF3455 are separated by 16 nucleotides GFF3455 and GFF3456 overlap by 24 nucleotides GFF3456 and GFF3457 are separated by 2 nucleotides GFF3457 and GFF3458 are separated by 229 nucleotides
GFF3453 - Periplasmic chorismate mutase I precursor (EC 5.4.99.5), at 211,905 to 212,450
GFF3453
GFF3454 - Putative transport protein, at 212,647 to 213,285
GFF3454
GFF3455 - FIG01046394: hypothetical protein, at 213,302 to 213,481
GFF3455
GFF3456 - FIG074102: hypothetical protein, at 213,458 to 213,820
GFF3456
GFF3457 - FIG074102: hypothetical protein, at 213,823 to 214,005
GFF3457
GFF3458 - Putative membrane protein, at 214,235 to 214,876
GFF3458
Position (kb)
213
214 Strain fitness (log2 ratio)
-1
0
1 at 212.448 kb on + strand at 212.477 kb on - strand at 212.623 kb on - strand at 212.660 kb on - strand at 212.776 kb on + strand, within GFF3454 at 212.777 kb on - strand, within GFF3454 at 212.903 kb on - strand, within GFF3454 at 212.911 kb on + strand, within GFF3454 at 212.911 kb on + strand, within GFF3454 at 213.818 kb on + strand at 213.838 kb on + strand at 213.840 kb on + strand at 213.841 kb on - strand at 214.108 kb on + strand at 214.113 kb on - strand at 214.228 kb on + strand at 214.270 kb on + strand at 214.270 kb on + strand at 214.409 kb on - strand, within GFF3458 at 214.409 kb on - strand, within GFF3458 at 214.409 kb on - strand, within GFF3458 at 214.411 kb on - strand, within GFF3458
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 212,448 + +0.0 212,477 - +0.4 212,623 - +0.0 212,660 - +0.9 212,776 + GFF3454 0.20 -0.6 212,777 - GFF3454 0.20 -0.7 212,903 - GFF3454 0.40 +0.4 212,911 + GFF3454 0.41 -0.2 212,911 + GFF3454 0.41 -0.3 213,818 + -0.0 213,838 + -1.2 213,840 + -0.6 213,841 - -0.1 214,108 + +0.2 214,113 - +0.3 214,228 + +0.4 214,270 + +0.1 214,270 + -0.1 214,409 - GFF3458 0.27 +0.3 214,409 - GFF3458 0.27 -0.1 214,409 - GFF3458 0.27 -1.5 214,411 - GFF3458 0.27 -0.3
Or see this region's nucleotide sequence