Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3012 and GFF3013 are separated by 380 nucleotides GFF3013 and GFF3014 are separated by 265 nucleotides
GFF3012 - 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2), at 27,436 to 28,032
GFF3012
GFF3013 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 28,413 to 29,645
GFF3013
GFF3014 - Ribose 5-phosphate isomerase A (EC 5.3.1.6), at 29,911 to 30,570
GFF3014
Position (kb)
28
29
30 Strain fitness (log2 ratio)
-1
0
1 at 27.651 kb on - strand, within GFF3012 at 27.724 kb on + strand, within GFF3012 at 27.891 kb on + strand, within GFF3012 at 27.891 kb on + strand, within GFF3012 at 27.892 kb on - strand, within GFF3012 at 27.915 kb on + strand, within GFF3012 at 27.916 kb on - strand, within GFF3012 at 27.923 kb on + strand, within GFF3012 at 27.985 kb on - strand at 28.125 kb on + strand at 28.125 kb on + strand at 28.128 kb on + strand at 28.173 kb on + strand at 28.173 kb on + strand at 28.200 kb on - strand at 28.258 kb on - strand at 28.268 kb on + strand at 28.276 kb on + strand at 28.298 kb on - strand at 28.301 kb on + strand at 28.302 kb on - strand at 28.333 kb on + strand at 28.333 kb on + strand at 28.333 kb on + strand at 28.334 kb on - strand at 28.498 kb on - strand at 28.507 kb on - strand at 28.513 kb on + strand at 28.557 kb on - strand, within GFF3013 at 28.706 kb on + strand, within GFF3013 at 28.706 kb on + strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.707 kb on - strand, within GFF3013 at 28.829 kb on + strand, within GFF3013 at 28.915 kb on - strand, within GFF3013 at 29.460 kb on - strand, within GFF3013 at 29.537 kb on - strand at 29.709 kb on + strand at 29.709 kb on + strand at 29.709 kb on + strand at 29.709 kb on + strand at 29.710 kb on - strand at 29.897 kb on + strand at 30.571 kb on - strand at 30.609 kb on - strand at 30.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 27,651 - GFF3012 0.36 +0.4 27,724 + GFF3012 0.48 -0.1 27,891 + GFF3012 0.76 -0.1 27,891 + GFF3012 0.76 -0.2 27,892 - GFF3012 0.76 +0.1 27,915 + GFF3012 0.80 -0.3 27,916 - GFF3012 0.80 -1.1 27,923 + GFF3012 0.82 -0.2 27,985 - +0.2 28,125 + +0.3 28,125 + -0.1 28,128 + +1.7 28,173 + +1.1 28,173 + +0.2 28,200 - -0.4 28,258 - +0.4 28,268 + -1.4 28,276 + +0.6 28,298 - +0.5 28,301 + +1.5 28,302 - +0.1 28,333 + -0.0 28,333 + +0.3 28,333 + -0.5 28,334 - +0.5 28,498 - +0.2 28,507 - -0.5 28,513 + +0.2 28,557 - GFF3013 0.12 +0.7 28,706 + GFF3013 0.24 +0.2 28,706 + GFF3013 0.24 -0.3 28,707 - GFF3013 0.24 -0.3 28,707 - GFF3013 0.24 +0.5 28,707 - GFF3013 0.24 +0.1 28,829 + GFF3013 0.34 +0.6 28,915 - GFF3013 0.41 +0.1 29,460 - GFF3013 0.85 +0.4 29,537 - +0.3 29,709 + +0.4 29,709 + -0.3 29,709 + +0.1 29,709 + +1.3 29,710 - +0.7 29,897 + -0.3 30,571 - -0.6 30,609 - -0.4 30,643 - +0.1
Or see this region's nucleotide sequence