Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2865 and GFF2866 are separated by 162 nucleotides GFF2866 and GFF2867 are separated by 485 nucleotides GFF2867 and GFF2868 are separated by 96 nucleotides GFF2868 and GFF2869 are separated by 392 nucleotides
GFF2865 - Mobile element protein, at 382,126 to 382,983
GFF2865
GFF2866 - Phage integrase, at 383,146 to 383,475
GFF2866
GFF2867 - hypothetical protein, at 383,961 to 384,161
GFF2867
GFF2868 - hypothetical protein, at 384,258 to 384,386
GFF2868
GFF2869 - Permease of the drug/metabolite transporter (DMT) superfamily, at 384,779 to 385,693
GFF2869
Position (kb)
383
384
385 Strain fitness (log2 ratio)
-2
-1
0
1 at 383.083 kb on + strand at 383.084 kb on - strand at 383.410 kb on - strand, within GFF2866 at 383.410 kb on - strand, within GFF2866 at 383.876 kb on - strand at 383.934 kb on + strand at 383.958 kb on + strand at 384.005 kb on + strand, within GFF2867 at 384.053 kb on + strand, within GFF2867 at 384.053 kb on + strand, within GFF2867 at 384.144 kb on - strand at 384.315 kb on + strand, within GFF2868 at 384.377 kb on + strand at 384.404 kb on - strand at 384.575 kb on + strand at 385.085 kb on - strand, within GFF2869
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 383,083 + -0.0 383,084 - +0.5 383,410 - GFF2866 0.80 +0.2 383,410 - GFF2866 0.80 -0.3 383,876 - +0.3 383,934 + -1.4 383,958 + -0.6 384,005 + GFF2867 0.22 -1.9 384,053 + GFF2867 0.46 -1.1 384,053 + GFF2867 0.46 -0.1 384,144 - +1.1 384,315 + GFF2868 0.44 -0.3 384,377 + +0.0 384,404 - +0.4 384,575 + +0.3 385,085 - GFF2869 0.33 -0.3
Or see this region's nucleotide sequence