Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF254 and GFF255 are separated by 63 nucleotides GFF255 and GFF256 are separated by 199 nucleotides GFF256 and GFF257 are separated by 79 nucleotides
GFF254 - 5-methyltetrahydropteroyltriglutamate-- homocysteine methyltransferase (EC 2.1.1.14), at 28,596 to 30,860
GFF254
GFF255 - hypothetical protein, at 30,924 to 31,052
GFF255
GFF256 - Putative arylsulfatase regulatory protein, at 31,252 to 32,547
GFF256
GFF257 - Putative carboxymethylenebutenolidase (EC 3.1.1.45), at 32,627 to 33,382
GFF257
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 30.381 kb on - strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.525 kb on + strand, within GFF254 at 30.738 kb on - strand at 30.738 kb on - strand at 30.858 kb on + strand at 30.859 kb on - strand at 31.031 kb on - strand, within GFF255 at 31.099 kb on + strand at 31.099 kb on + strand at 31.175 kb on + strand at 31.175 kb on + strand at 31.176 kb on - strand at 31.275 kb on - strand at 31.318 kb on + strand at 31.318 kb on + strand at 31.327 kb on + strand at 31.328 kb on - strand at 31.328 kb on - strand at 31.401 kb on + strand, within GFF256 at 31.401 kb on + strand, within GFF256 at 31.403 kb on + strand, within GFF256 at 31.413 kb on + strand, within GFF256 at 31.509 kb on - strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.646 kb on + strand, within GFF256 at 31.666 kb on - strand, within GFF256 at 31.741 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.744 kb on - strand, within GFF256 at 31.824 kb on + strand, within GFF256 at 31.845 kb on - strand, within GFF256 at 31.864 kb on - strand, within GFF256 at 31.875 kb on - strand, within GFF256 at 31.878 kb on + strand, within GFF256 at 32.002 kb on + strand, within GFF256 at 32.003 kb on - strand, within GFF256 at 32.064 kb on - strand, within GFF256 at 32.186 kb on + strand, within GFF256 at 32.190 kb on + strand, within GFF256 at 32.191 kb on - strand, within GFF256 at 32.273 kb on + strand, within GFF256 at 32.274 kb on - strand, within GFF256 at 32.334 kb on + strand, within GFF256 at 32.338 kb on + strand, within GFF256 at 32.354 kb on - strand, within GFF256 at 32.356 kb on - strand, within GFF256 at 32.383 kb on + strand, within GFF256 at 32.384 kb on - strand, within GFF256 at 32.384 kb on - strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.385 kb on + strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.386 kb on - strand, within GFF256 at 32.487 kb on + strand at 32.538 kb on + strand at 32.539 kb on - strand at 32.545 kb on + strand at 32.549 kb on + strand at 32.549 kb on + strand at 32.615 kb on - strand at 32.615 kb on - strand at 32.726 kb on - strand, within GFF257 at 32.749 kb on - strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.951 kb on + strand, within GFF257 at 32.952 kb on - strand, within GFF257 at 32.973 kb on + strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.974 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 32.984 kb on - strand, within GFF257 at 33.095 kb on + strand, within GFF257 at 33.128 kb on + strand, within GFF257 at 33.129 kb on - strand, within GFF257 at 33.130 kb on + strand, within GFF257 at 33.316 kb on + strand at 33.443 kb on - strand at 33.475 kb on + strand at 33.479 kb on + strand at 33.479 kb on + strand at 33.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 30,381 - GFF254 0.79 +0.1 30,525 + GFF254 0.85 +0.1 30,525 + GFF254 0.85 -0.2 30,738 - +1.5 30,738 - +0.4 30,858 + +0.1 30,859 - -0.0 31,031 - GFF255 0.83 -0.2 31,099 + +0.3 31,099 + +0.5 31,175 + +0.6 31,175 + +0.2 31,176 - -1.0 31,275 - +0.8 31,318 + -0.1 31,318 + +0.5 31,327 + +0.6 31,328 - +0.1 31,328 - -1.3 31,401 + GFF256 0.11 +0.1 31,401 + GFF256 0.11 -0.1 31,403 + GFF256 0.12 +0.2 31,413 + GFF256 0.12 +0.8 31,509 - GFF256 0.20 +0.4 31,646 + GFF256 0.30 +0.0 31,646 + GFF256 0.30 +0.8 31,666 - GFF256 0.32 +0.7 31,741 - GFF256 0.38 +0.7 31,744 - GFF256 0.38 +0.2 31,744 - GFF256 0.38 +0.0 31,824 + GFF256 0.44 +0.1 31,845 - GFF256 0.46 +1.0 31,864 - GFF256 0.47 -1.7 31,875 - GFF256 0.48 -0.2 31,878 + GFF256 0.48 +0.2 32,002 + GFF256 0.58 +0.2 32,003 - GFF256 0.58 +0.2 32,064 - GFF256 0.63 +0.6 32,186 + GFF256 0.72 +0.3 32,190 + GFF256 0.72 +0.1 32,191 - GFF256 0.72 +0.7 32,273 + GFF256 0.79 +0.3 32,274 - GFF256 0.79 +0.2 32,334 + GFF256 0.83 +0.1 32,338 + GFF256 0.84 +0.1 32,354 - GFF256 0.85 +0.2 32,356 - GFF256 0.85 +0.8 32,383 + GFF256 0.87 -1.1 32,384 - GFF256 0.87 -0.6 32,384 - GFF256 0.87 +0.2 32,385 + GFF256 0.87 -1.3 32,385 + GFF256 0.87 -3.2 32,385 + GFF256 0.87 +0.8 32,386 - GFF256 0.88 +0.3 32,386 - GFF256 0.88 +0.5 32,386 - GFF256 0.88 +0.4 32,487 + +0.2 32,538 + -0.0 32,539 - +0.3 32,545 + +0.2 32,549 + +0.9 32,549 + -0.1 32,615 - +0.0 32,615 - +0.0 32,726 - GFF257 0.13 +0.6 32,749 - GFF257 0.16 -0.1 32,951 + GFF257 0.43 -0.3 32,951 + GFF257 0.43 +0.5 32,952 - GFF257 0.43 +1.5 32,973 + GFF257 0.46 -0.3 32,974 - GFF257 0.46 +1.0 32,974 - GFF257 0.46 +0.4 32,984 - GFF257 0.47 +0.1 32,984 - GFF257 0.47 +0.2 33,095 + GFF257 0.62 +0.4 33,128 + GFF257 0.66 +0.4 33,129 - GFF257 0.66 +0.2 33,130 + GFF257 0.67 +0.1 33,316 + +0.1 33,443 - +0.1 33,475 + -0.2 33,479 + +0.4 33,479 + -0.4 33,481 + +0.1
Or see this region's nucleotide sequence