Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1925 and GFF1926 are separated by 210 nucleotides GFF1926 and GFF1927 are separated by 35 nucleotides
GFF1925 - Diaminopimelate decarboxylase (EC 4.1.1.20), at 117,655 to 119,052
GFF1925
GFF1926 - Arginine utilization regulatory protein RocR, at 119,263 to 120,690
GFF1926
GFF1927 - Amidophosphoribosyltransferase (EC 2.4.2.14), at 120,726 to 122,243
GFF1927
Position (kb)
119
120
121 Strain fitness (log2 ratio)
-1
0
1 at 118.404 kb on + strand, within GFF1925 at 118.405 kb on - strand, within GFF1925 at 118.596 kb on + strand, within GFF1925 at 118.629 kb on + strand, within GFF1925 at 118.629 kb on + strand, within GFF1925 at 118.630 kb on - strand, within GFF1925 at 118.656 kb on - strand, within GFF1925 at 118.878 kb on + strand, within GFF1925 at 118.908 kb on - strand, within GFF1925 at 119.018 kb on - strand at 119.018 kb on - strand at 119.226 kb on - strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.260 kb on + strand at 119.262 kb on + strand at 119.263 kb on - strand at 119.263 kb on - strand at 119.350 kb on - strand at 119.374 kb on + strand at 119.375 kb on - strand at 119.375 kb on - strand at 119.406 kb on + strand, within GFF1926 at 119.406 kb on + strand, within GFF1926 at 119.407 kb on - strand, within GFF1926 at 119.433 kb on + strand, within GFF1926 at 119.433 kb on + strand, within GFF1926 at 119.472 kb on + strand, within GFF1926 at 119.628 kb on + strand, within GFF1926 at 119.629 kb on - strand, within GFF1926 at 119.775 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.837 kb on + strand, within GFF1926 at 119.901 kb on + strand, within GFF1926 at 119.916 kb on + strand, within GFF1926 at 120.282 kb on + strand, within GFF1926 at 120.548 kb on - strand at 120.560 kb on + strand at 120.658 kb on + strand at 120.659 kb on - strand at 120.684 kb on + strand at 120.685 kb on - strand at 120.794 kb on + strand at 120.794 kb on + strand at 120.795 kb on - strand at 120.831 kb on + strand at 120.847 kb on + strand at 120.847 kb on + strand at 120.847 kb on + strand at 120.847 kb on + strand at 120.848 kb on - strand at 120.848 kb on - strand at 121.027 kb on + strand, within GFF1927 at 121.028 kb on - strand, within GFF1927 at 121.239 kb on - strand, within GFF1927 at 121.244 kb on + strand, within GFF1927 at 121.245 kb on - strand, within GFF1927 at 121.341 kb on - strand, within GFF1927 at 121.548 kb on - strand, within GFF1927 at 121.548 kb on - strand, within GFF1927 at 121.549 kb on + strand, within GFF1927 at 121.549 kb on + strand, within GFF1927 at 121.549 kb on + strand, within GFF1927 at 121.550 kb on - strand, within GFF1927 at 121.550 kb on - strand, within GFF1927 at 121.550 kb on - strand, within GFF1927 at 121.550 kb on - strand, within GFF1927 at 121.550 kb on - strand, within GFF1927 at 121.554 kb on - strand, within GFF1927 at 121.628 kb on + strand, within GFF1927 at 121.629 kb on - strand, within GFF1927 at 121.630 kb on + strand, within GFF1927 at 121.630 kb on + strand, within GFF1927 at 121.631 kb on - strand, within GFF1927
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 118,404 + GFF1925 0.54 +1.6 118,405 - GFF1925 0.54 -0.1 118,596 + GFF1925 0.67 +0.1 118,629 + GFF1925 0.70 +0.3 118,629 + GFF1925 0.70 -0.0 118,630 - GFF1925 0.70 +0.0 118,656 - GFF1925 0.72 -0.0 118,878 + GFF1925 0.87 +0.6 118,908 - GFF1925 0.90 +0.5 119,018 - -0.1 119,018 - -0.0 119,226 - +0.8 119,260 + +0.2 119,260 + +0.5 119,260 + +0.0 119,260 + +0.1 119,262 + -0.3 119,263 - -0.3 119,263 - +0.2 119,350 - +0.9 119,374 + +0.6 119,375 - +1.3 119,375 - +0.3 119,406 + GFF1926 0.10 +0.1 119,406 + GFF1926 0.10 -0.1 119,407 - GFF1926 0.10 -1.0 119,433 + GFF1926 0.12 +0.8 119,433 + GFF1926 0.12 +0.0 119,472 + GFF1926 0.15 +0.5 119,628 + GFF1926 0.26 -0.1 119,629 - GFF1926 0.26 +0.5 119,775 + GFF1926 0.36 +1.3 119,837 + GFF1926 0.40 -0.1 119,837 + GFF1926 0.40 +0.5 119,837 + GFF1926 0.40 -0.1 119,901 + GFF1926 0.45 +0.5 119,916 + GFF1926 0.46 +0.6 120,282 + GFF1926 0.71 -0.1 120,548 - +0.6 120,560 + -0.3 120,658 + -0.0 120,659 - -0.0 120,684 + +0.2 120,685 - +0.1 120,794 + +0.3 120,794 + -0.4 120,795 - +0.2 120,831 + -0.1 120,847 + +0.4 120,847 + +0.3 120,847 + +0.2 120,847 + -0.1 120,848 - +0.2 120,848 - +0.4 121,027 + GFF1927 0.20 -1.0 121,028 - GFF1927 0.20 +0.1 121,239 - GFF1927 0.34 -0.0 121,244 + GFF1927 0.34 +0.1 121,245 - GFF1927 0.34 +0.2 121,341 - GFF1927 0.41 +1.2 121,548 - GFF1927 0.54 +0.2 121,548 - GFF1927 0.54 -0.0 121,549 + GFF1927 0.54 +0.0 121,549 + GFF1927 0.54 +0.1 121,549 + GFF1927 0.54 -0.1 121,550 - GFF1927 0.54 -0.1 121,550 - GFF1927 0.54 +0.4 121,550 - GFF1927 0.54 -0.1 121,550 - GFF1927 0.54 -0.1 121,550 - GFF1927 0.54 +0.4 121,554 - GFF1927 0.55 +0.1 121,628 + GFF1927 0.59 +1.0 121,629 - GFF1927 0.59 +0.7 121,630 + GFF1927 0.60 -0.4 121,630 + GFF1927 0.60 +0.8 121,631 - GFF1927 0.60 -0.1
Or see this region's nucleotide sequence