Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1529 and GFF1530 are separated by 26 nucleotides GFF1530 and GFF1531 are separated by 10 nucleotides GFF1531 and GFF1532 are separated by 333 nucleotides
GFF1529 - PTS system, galactose-inducible IIB component (EC 2.7.1.69) / PTS system, galactose-inducible IIC component (EC 2.7.1.69), at 32,720 to 34,147
GFF1529
GFF1530 - Tagatose-6-phosphate kinase (EC 2.7.1.144) / 1-phosphofructokinase (EC 2.7.1.56), at 34,174 to 35,088
GFF1530
GFF1531 - Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40), at 35,099 to 35,953
GFF1531
GFF1532 - Transcriptional repressor of aga operon, at 36,287 to 37,120
GFF1532
Position (kb) 35
36 Strain fitness (log2 ratio) -1
0
1 at 34.205 kb on - strand at 34.232 kb on - strand at 34.415 kb on + strand, within GFF1530 at 35.040 kb on + strand at 35.047 kb on - strand at 35.064 kb on + strand at 35.406 kb on + strand, within GFF1531 at 35.407 kb on - strand, within GFF1531 at 35.519 kb on + strand, within GFF1531 at 35.520 kb on - strand, within GFF1531 at 35.791 kb on + strand, within GFF1531 at 35.792 kb on - strand, within GFF1531 at 35.792 kb on - strand, within GFF1531 at 36.227 kb on + strand at 36.592 kb on + strand, within GFF1532 at 36.593 kb on - strand, within GFF1532 at 36.836 kb on - strand, within GFF1532
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 34,205 - -0.4 34,232 - +0.6 34,415 + GFF1530 0.26 +0.0 35,040 + +0.3 35,047 - -0.5 35,064 + -0.1 35,406 + GFF1531 0.36 +0.4 35,407 - GFF1531 0.36 +0.6 35,519 + GFF1531 0.49 +0.2 35,520 - GFF1531 0.49 +1.0 35,791 + GFF1531 0.81 +0.1 35,792 - GFF1531 0.81 +0.2 35,792 - GFF1531 0.81 +0.1 36,227 + -0.3 36,592 + GFF1532 0.37 +0.3 36,593 - GFF1532 0.37 +0.0 36,836 - GFF1532 0.66 -0.7
Or see this region's nucleotide sequence