Experiment: LB_plus_SM_buffer
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1396 and GFF1397 are separated by 440 nucleotides GFF1397 and GFF1398 are separated by 63 nucleotides GFF1398 and GFF1399 overlap by 4 nucleotides
GFF1396 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 40,292 to 41,941
GFF1396
GFF1397 - YjbE secreted protein, at 42,382 to 42,627
GFF1397
GFF1398 - YjbF outer membrane lipoprotein, at 42,691 to 43,329
GFF1398
GFF1399 - YjbG polysaccharide synthesis-related protein, at 43,326 to 44,063
GFF1399
Position (kb) 42
43 Strain fitness (log2 ratio) -2
-1
0
1 at 41.416 kb on + strand, within GFF1396 at 41.477 kb on - strand, within GFF1396 at 41.705 kb on - strand, within GFF1396 at 41.756 kb on + strand, within GFF1396 at 41.756 kb on + strand, within GFF1396 at 41.757 kb on - strand, within GFF1396 at 41.757 kb on - strand, within GFF1396 at 41.801 kb on - strand at 41.893 kb on + strand at 41.894 kb on - strand at 41.894 kb on - strand at 41.899 kb on + strand at 41.921 kb on + strand at 41.964 kb on - strand at 41.967 kb on + strand at 41.968 kb on - strand at 41.968 kb on - strand at 42.027 kb on - strand at 42.032 kb on - strand at 42.400 kb on + strand at 42.533 kb on + strand, within GFF1397 at 42.533 kb on + strand, within GFF1397 at 42.534 kb on - strand, within GFF1397 at 42.641 kb on - strand at 42.725 kb on + strand at 42.835 kb on + strand, within GFF1398 at 43.005 kb on + strand, within GFF1398 at 43.005 kb on + strand, within GFF1398 at 43.125 kb on + strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.185 kb on - strand, within GFF1398 at 43.271 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer remove 41,416 + GFF1396 0.68 -1.4 41,477 - GFF1396 0.72 -0.4 41,705 - GFF1396 0.86 -1.3 41,756 + GFF1396 0.89 -1.8 41,756 + GFF1396 0.89 -0.1 41,757 - GFF1396 0.89 -1.5 41,757 - GFF1396 0.89 -1.8 41,801 - -1.6 41,893 + -1.3 41,894 - -1.0 41,894 - -0.9 41,899 + -0.2 41,921 + -1.1 41,964 - +0.2 41,967 + -0.0 41,968 - -0.1 41,968 - +0.3 42,027 - +0.6 42,032 - -0.1 42,400 + +0.4 42,533 + GFF1397 0.61 +0.1 42,533 + GFF1397 0.61 +0.7 42,534 - GFF1397 0.62 +0.2 42,641 - +0.4 42,725 + -0.1 42,835 + GFF1398 0.23 +0.6 43,005 + GFF1398 0.49 +0.6 43,005 + GFF1398 0.49 +0.3 43,125 + GFF1398 0.68 +0.5 43,185 - GFF1398 0.77 +0.0 43,185 - GFF1398 0.77 -0.2 43,271 - +0.1
Or see this region's nucleotide sequence