Experiment: m.b. Nalidixic 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Echvi_0322 and Echvi_0323 are separated by 70 nucleotides Echvi_0323 and Echvi_0324 are separated by 218 nucleotides
Echvi_0322: Echvi_0322 - Glycerol-3-phosphate dehydrogenase, at 336,329 to 337,393
_0322
Echvi_0323: Echvi_0323 - Gluconolactonase, at 337,464 to 338,357
_0323
Echvi_0324: Echvi_0324 - Uncharacterized protein involved in methicillin resistance, at 338,576 to 339,670
_0324
Position (kb) 337
338
339 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 336.580 kb on - strand, within Echvi_0322 at 336.912 kb on - strand, within Echvi_0322 at 337.154 kb on + strand, within Echvi_0322 at 337.327 kb on + strand at 337.346 kb on + strand at 337.346 kb on + strand at 337.349 kb on + strand at 337.350 kb on - strand at 337.626 kb on + strand, within Echvi_0323 at 337.626 kb on + strand, within Echvi_0323 at 337.626 kb on + strand, within Echvi_0323 at 337.656 kb on + strand, within Echvi_0323 at 337.657 kb on - strand, within Echvi_0323 at 337.657 kb on - strand, within Echvi_0323 at 337.692 kb on + strand, within Echvi_0323 at 337.693 kb on - strand, within Echvi_0323 at 337.725 kb on + strand, within Echvi_0323 at 337.889 kb on + strand, within Echvi_0323 at 337.902 kb on + strand, within Echvi_0323 at 337.903 kb on - strand, within Echvi_0323 at 337.956 kb on + strand, within Echvi_0323 at 337.957 kb on - strand, within Echvi_0323 at 338.080 kb on - strand, within Echvi_0323 at 338.126 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.179 kb on - strand, within Echvi_0323 at 338.323 kb on - strand at 338.323 kb on - strand at 338.336 kb on - strand at 338.355 kb on + strand at 338.356 kb on - strand at 338.378 kb on + strand at 338.395 kb on - strand at 338.414 kb on + strand at 338.414 kb on + strand at 338.415 kb on - strand at 338.415 kb on - strand at 338.415 kb on - strand at 338.417 kb on + strand at 338.420 kb on + strand at 338.420 kb on + strand at 338.420 kb on + strand at 338.434 kb on + strand at 338.435 kb on - strand at 338.435 kb on - strand at 338.445 kb on - strand at 338.478 kb on - strand at 338.491 kb on + strand at 338.497 kb on + strand at 338.497 kb on + strand at 338.611 kb on - strand at 338.745 kb on + strand, within Echvi_0324 at 338.746 kb on - strand, within Echvi_0324 at 338.799 kb on + strand, within Echvi_0324 at 338.799 kb on + strand, within Echvi_0324 at 338.848 kb on - strand, within Echvi_0324 at 338.982 kb on + strand, within Echvi_0324 at 339.016 kb on - strand, within Echvi_0324 at 339.016 kb on - strand, within Echvi_0324 at 339.094 kb on + strand, within Echvi_0324 at 339.095 kb on - strand, within Echvi_0324 at 339.095 kb on - strand, within Echvi_0324 at 339.096 kb on + strand, within Echvi_0324 at 339.096 kb on + strand, within Echvi_0324 at 339.097 kb on - strand, within Echvi_0324 at 339.097 kb on - strand, within Echvi_0324 at 339.097 kb on - strand, within Echvi_0324 at 339.108 kb on + strand, within Echvi_0324 at 339.109 kb on - strand, within Echvi_0324 at 339.109 kb on - strand at 339.120 kb on + strand, within Echvi_0324 at 339.224 kb on + strand, within Echvi_0324 at 339.282 kb on + strand, within Echvi_0324 at 339.353 kb on + strand, within Echvi_0324 at 339.353 kb on + strand, within Echvi_0324
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Nalidixic 0.008 mg/ml remove 336,580 - Echvi_0322 0.24 +4.1 336,912 - Echvi_0322 0.55 +1.3 337,154 + Echvi_0322 0.77 -0.4 337,327 + -1.7 337,346 + -1.7 337,346 + -2.7 337,349 + +1.2 337,350 - +0.7 337,626 + Echvi_0323 0.18 -1.3 337,626 + Echvi_0323 0.18 +2.7 337,626 + Echvi_0323 0.18 -0.6 337,656 + Echvi_0323 0.21 +0.7 337,657 - Echvi_0323 0.22 -1.8 337,657 - Echvi_0323 0.22 +0.3 337,692 + Echvi_0323 0.26 +1.0 337,693 - Echvi_0323 0.26 -2.2 337,725 + Echvi_0323 0.29 -0.8 337,889 + Echvi_0323 0.48 -1.1 337,902 + Echvi_0323 0.49 -1.1 337,903 - Echvi_0323 0.49 +0.2 337,956 + Echvi_0323 0.55 +0.9 337,957 - Echvi_0323 0.55 -0.6 338,080 - Echvi_0323 0.69 -0.2 338,126 - Echvi_0323 0.74 -0.1 338,179 - Echvi_0323 0.80 -1.2 338,179 - Echvi_0323 0.80 -1.2 338,179 - Echvi_0323 0.80 +0.6 338,179 - Echvi_0323 0.80 +1.3 338,323 - -0.0 338,323 - -0.2 338,336 - -1.9 338,355 + +0.4 338,356 - -0.5 338,378 + -2.2 338,395 - +0.4 338,414 + -1.5 338,414 + -1.3 338,415 - +0.2 338,415 - -0.8 338,415 - -1.2 338,417 + -1.4 338,420 + -0.1 338,420 + -0.2 338,420 + +0.2 338,434 + +0.2 338,435 - +0.4 338,435 - -0.8 338,445 - -0.2 338,478 - -0.2 338,491 + -0.1 338,497 + -0.7 338,497 + -1.0 338,611 - +1.2 338,745 + Echvi_0324 0.15 +1.0 338,746 - Echvi_0324 0.16 -0.8 338,799 + Echvi_0324 0.20 -0.2 338,799 + Echvi_0324 0.20 -0.1 338,848 - Echvi_0324 0.25 +0.7 338,982 + Echvi_0324 0.37 -0.2 339,016 - Echvi_0324 0.40 -1.6 339,016 - Echvi_0324 0.40 +0.3 339,094 + Echvi_0324 0.47 +1.2 339,095 - Echvi_0324 0.47 +0.3 339,095 - Echvi_0324 0.47 +0.2 339,096 + Echvi_0324 0.47 +0.2 339,096 + Echvi_0324 0.47 -1.3 339,097 - Echvi_0324 0.48 +0.4 339,097 - Echvi_0324 0.48 +0.2 339,097 - Echvi_0324 0.48 +0.6 339,108 + Echvi_0324 0.49 +1.2 339,109 - Echvi_0324 0.49 +0.7 339,109 - -0.6 339,120 + Echvi_0324 0.50 +4.4 339,224 + Echvi_0324 0.59 -0.7 339,282 + Echvi_0324 0.64 -1.7 339,353 + Echvi_0324 0.71 -0.1 339,353 + Echvi_0324 0.71 -0.3
Or see this region's nucleotide sequence