Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfL and ymfM overlap by 4 nucleotides ymfM and oweE are separated by 9 nucleotides oweE and aaaE are separated by 0 nucleotides aaaE and ymfR are separated by 11 nucleotides ymfR and ymfO overlap by 1 nucleotides ymfO and ymfP are separated by 0 nucleotides
b1147: ymfL - e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 1,202,491 to 1,203,048
ymfL
b1148: ymfM - e14 prophage; predicted protein (NCBI), at 1,203,045 to 1,203,383
ymfM
b4692: oweE - no description, at 1,203,393 to 1,203,626
oweE
b4693: aaaE - no description, at 1,203,627 to 1,204,760
aaaE
b1150: ymfR - e14 prophage; predicted protein (NCBI), at 1,204,772 to 1,204,954
ymfR
b1151: ymfO - e14 prophage; conserved protein (RefSeq), at 1,204,954 to 1,205,365
ymfO
b1152: ymfP - orf, hypothetical protein (VIMSS), at 1,205,366 to 1,206,145
ymfP
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 1202.825 kb on - strand, within ymfL at 1202.869 kb on - strand, within ymfL at 1202.869 kb on - strand, within ymfL at 1202.897 kb on - strand, within ymfL at 1202.897 kb on - strand, within ymfL at 1202.909 kb on - strand, within ymfL at 1203.060 kb on + strand at 1203.060 kb on + strand at 1203.173 kb on - strand, within ymfM at 1203.180 kb on - strand, within ymfM at 1203.189 kb on - strand, within ymfM at 1203.189 kb on - strand, within ymfM at 1203.378 kb on + strand at 1203.378 kb on + strand at 1203.435 kb on + strand, within oweE at 1203.490 kb on + strand, within oweE at 1203.556 kb on + strand, within oweE at 1203.617 kb on + strand at 1203.617 kb on + strand at 1203.646 kb on - strand at 1203.684 kb on - strand at 1203.684 kb on - strand at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.880 kb on - strand, within aaaE at 1203.891 kb on + strand, within aaaE at 1204.010 kb on + strand, within aaaE at 1204.018 kb on - strand, within aaaE at 1204.078 kb on - strand, within aaaE at 1204.084 kb on + strand, within aaaE at 1204.196 kb on + strand, within aaaE at 1204.204 kb on - strand, within aaaE at 1204.237 kb on - strand, within aaaE at 1204.391 kb on - strand, within aaaE at 1204.420 kb on - strand, within aaaE at 1204.462 kb on + strand, within aaaE at 1204.841 kb on - strand, within ymfR at 1204.996 kb on + strand, within ymfO at 1205.042 kb on - strand, within ymfO at 1205.128 kb on + strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.409 kb on + strand at 1205.409 kb on + strand at 1205.435 kb on - strand at 1205.437 kb on - strand at 1205.474 kb on + strand, within ymfP at 1205.474 kb on + strand, within ymfP at 1205.630 kb on + strand, within ymfP at 1205.661 kb on - strand, within ymfP at 1205.709 kb on - strand, within ymfP at 1205.740 kb on - strand, within ymfP
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 1,202,825 - ymfL b1147 0.60 -1.4 1,202,869 - ymfL b1147 0.68 -1.1 1,202,869 - ymfL b1147 0.68 +1.1 1,202,897 - ymfL b1147 0.73 +2.8 1,202,897 - ymfL b1147 0.73 +1.3 1,202,909 - ymfL b1147 0.75 +1.3 1,203,060 + -1.1 1,203,060 + +0.1 1,203,173 - ymfM b1148 0.38 -0.1 1,203,180 - ymfM b1148 0.40 +5.2 1,203,189 - ymfM b1148 0.42 -0.3 1,203,189 - ymfM b1148 0.42 +1.3 1,203,378 + +0.3 1,203,378 + -0.1 1,203,435 + oweE b4692 0.18 +1.7 1,203,490 + oweE b4692 0.41 -1.1 1,203,556 + oweE b4692 0.70 +0.5 1,203,617 + +1.5 1,203,617 + +0.8 1,203,646 - +0.8 1,203,684 - +1.3 1,203,684 - -1.7 1,203,872 + aaaE b4693 0.22 -0.3 1,203,872 + aaaE b4693 0.22 +1.3 1,203,872 + aaaE b4693 0.22 +0.8 1,203,872 + aaaE b4693 0.22 +1.7 1,203,880 - aaaE b4693 0.22 -1.7 1,203,891 + aaaE b4693 0.23 +0.3 1,204,010 + aaaE b4693 0.34 -1.5 1,204,018 - aaaE b4693 0.34 -1.5 1,204,078 - aaaE b4693 0.40 -1.3 1,204,084 + aaaE b4693 0.40 +1.6 1,204,196 + aaaE b4693 0.50 +0.5 1,204,204 - aaaE b4693 0.51 -2.1 1,204,237 - aaaE b4693 0.54 -1.5 1,204,391 - aaaE b4693 0.67 -1.6 1,204,420 - aaaE b4693 0.70 -0.5 1,204,462 + aaaE b4693 0.74 -1.1 1,204,841 - ymfR b1150 0.38 +0.3 1,204,996 + ymfO b1151 0.10 -0.8 1,205,042 - ymfO b1151 0.21 -0.3 1,205,128 + ymfO b1151 0.42 -0.7 1,205,226 - ymfO b1151 0.66 +0.5 1,205,226 - ymfO b1151 0.66 -0.3 1,205,409 + +0.9 1,205,409 + -1.2 1,205,435 - -0.1 1,205,437 - -1.1 1,205,474 + ymfP b1152 0.14 +0.7 1,205,474 + ymfP b1152 0.14 -0.3 1,205,630 + ymfP b1152 0.34 +0.6 1,205,661 - ymfP b1152 0.38 -1.0 1,205,709 - ymfP b1152 0.44 +0.7 1,205,740 - ymfP b1152 0.48 -1.7
Or see this region's nucleotide sequence