Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgcE and sgcA are separated by 11 nucleotides sgcA and ryjB overlap by 4 nucleotides ryjB and sgcQ are separated by 44 nucleotides sgcQ and sgcC are separated by 12 nucleotides
b4301: sgcE - ribulose-phosphate 3-epimerase (NCBI), at 4,524,928 to 4,525,560
sgcE
b4302: sgcA - KpLE2 phage-like element; predicted phosphotransferase enzyme IIA component (NCBI), at 4,525,572 to 4,526,003
sgcA
b4624: ryjB - Novel sRNA, function unknown (RefSeq), at 4,526,000 to 4,526,089
ryjB
b4303: sgcQ - KpLE2 phage-like element; predicted nucleoside triphosphatase (NCBI), at 4,526,134 to 4,526,940
sgcQ
b4304: sgcC - KpLE2 phage-like element; predicted phosphotransferase enzyme IIC component (NCBI), at 4,526,953 to 4,528,266
sgcC
Position (kb)
4525
4526
4527 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4525.061 kb on - strand, within sgcE at 4525.078 kb on - strand, within sgcE at 4525.167 kb on - strand, within sgcE at 4525.217 kb on - strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.220 kb on + strand, within sgcE at 4525.290 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.315 kb on - strand, within sgcE at 4525.363 kb on + strand, within sgcE at 4525.462 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.492 kb on + strand, within sgcE at 4525.519 kb on + strand at 4525.549 kb on + strand at 4525.559 kb on + strand at 4525.565 kb on - strand at 4525.632 kb on - strand, within sgcA at 4525.632 kb on - strand, within sgcA at 4525.650 kb on + strand, within sgcA at 4525.663 kb on - strand, within sgcA at 4525.756 kb on + strand, within sgcA at 4525.761 kb on + strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.805 kb on - strand, within sgcA at 4525.844 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.888 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.896 kb on + strand, within sgcA at 4525.914 kb on + strand, within sgcA at 4525.971 kb on - strand at 4526.051 kb on + strand, within ryjB at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.140 kb on + strand at 4526.164 kb on + strand at 4526.174 kb on + strand at 4526.174 kb on + strand at 4526.254 kb on + strand, within sgcQ at 4526.254 kb on + strand, within sgcQ at 4526.257 kb on + strand, within sgcQ at 4526.298 kb on + strand, within sgcQ at 4526.338 kb on - strand at 4526.356 kb on + strand, within sgcQ at 4526.356 kb on + strand, within sgcQ at 4526.388 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.448 kb on + strand, within sgcQ at 4526.460 kb on + strand, within sgcQ at 4526.461 kb on + strand, within sgcQ at 4526.468 kb on - strand, within sgcQ at 4526.468 kb on - strand, within sgcQ at 4526.583 kb on - strand, within sgcQ at 4526.621 kb on - strand, within sgcQ at 4526.738 kb on + strand, within sgcQ at 4526.769 kb on - strand, within sgcQ at 4526.774 kb on + strand, within sgcQ at 4526.809 kb on + strand, within sgcQ at 4526.874 kb on + strand at 4526.885 kb on - strand at 4526.885 kb on - strand at 4526.895 kb on - strand at 4526.975 kb on + strand at 4526.975 kb on + strand at 4526.993 kb on - strand at 4526.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,525,061 - sgcE b4301 0.21 +0.2 4,525,078 - sgcE b4301 0.24 -2.1 4,525,167 - sgcE b4301 0.38 -2.2 4,525,217 - sgcE b4301 0.46 -1.1 4,525,220 + sgcE b4301 0.46 +1.0 4,525,220 + sgcE b4301 0.46 -0.6 4,525,290 - sgcE b4301 0.57 +0.2 4,525,315 - sgcE b4301 0.61 +1.1 4,525,315 - sgcE b4301 0.61 +1.2 4,525,363 + sgcE b4301 0.69 +0.1 4,525,462 + sgcE b4301 0.84 -0.9 4,525,492 + sgcE b4301 0.89 -1.4 4,525,492 + sgcE b4301 0.89 -2.4 4,525,519 + +1.0 4,525,549 + +0.4 4,525,559 + -0.8 4,525,565 - -1.9 4,525,632 - sgcA b4302 0.14 +1.7 4,525,632 - sgcA b4302 0.14 +0.4 4,525,650 + sgcA b4302 0.18 -0.4 4,525,663 - sgcA b4302 0.21 +0.2 4,525,756 + sgcA b4302 0.43 +0.1 4,525,761 + sgcA b4302 0.44 -1.2 4,525,805 - sgcA b4302 0.54 +1.6 4,525,805 - sgcA b4302 0.54 -1.4 4,525,844 + sgcA b4302 0.63 -1.6 4,525,888 + sgcA b4302 0.73 -0.3 4,525,888 + sgcA b4302 0.73 -0.5 4,525,896 + sgcA b4302 0.75 -2.5 4,525,896 + sgcA b4302 0.75 -1.8 4,525,914 + sgcA b4302 0.79 -0.9 4,525,971 - +1.7 4,526,051 + ryjB b4624 0.57 +2.5 4,526,140 + -0.9 4,526,140 + +1.4 4,526,140 + +0.4 4,526,164 + +1.0 4,526,174 + +2.3 4,526,174 + -1.6 4,526,254 + sgcQ b4303 0.15 -2.3 4,526,254 + sgcQ b4303 0.15 +2.5 4,526,257 + sgcQ b4303 0.15 +2.9 4,526,298 + sgcQ b4303 0.20 -0.4 4,526,338 - +1.4 4,526,356 + sgcQ b4303 0.28 +1.6 4,526,356 + sgcQ b4303 0.28 +0.4 4,526,388 + sgcQ b4303 0.31 +2.8 4,526,448 + sgcQ b4303 0.39 -0.2 4,526,448 + sgcQ b4303 0.39 -0.4 4,526,460 + sgcQ b4303 0.40 -0.3 4,526,461 + sgcQ b4303 0.41 -0.5 4,526,468 - sgcQ b4303 0.41 -0.2 4,526,468 - sgcQ b4303 0.41 +0.8 4,526,583 - sgcQ b4303 0.56 +0.4 4,526,621 - sgcQ b4303 0.60 +0.4 4,526,738 + sgcQ b4303 0.75 +1.1 4,526,769 - sgcQ b4303 0.79 +1.1 4,526,774 + sgcQ b4303 0.79 +0.9 4,526,809 + sgcQ b4303 0.84 -1.4 4,526,874 + -2.5 4,526,885 - +0.6 4,526,885 - -0.9 4,526,895 - +0.5 4,526,975 + +0.4 4,526,975 + -1.6 4,526,993 - -0.0 4,526,993 - -0.7
Or see this region's nucleotide sequence