Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylcI and nohB are separated by 388 nucleotides nohB and ylcF overlap by 26 nucleotides ylcF and tfaD overlap by 3 nucleotides tfaD and ybcY are separated by 54 nucleotides
b4589: ylcI - hypothetical protein (RefSeq), at 579,474 to 579,668
ylcI
b0560: nohB - DLP12 prophage; DNA packaging protein (NCBI), at 580,057 to 580,602
nohB
b4634: ylcF - no description, at 580,577 to 580,885
ylcF
b0561: tfaD - orf, hypothetical protein (VIMSS), at 580,883 to 581,320
tfaD
b0562: ybcY - orf, hypothetical protein (VIMSS), at 581,375 to 582,029
ybcY
Position (kb) 580
581 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 580.052 kb on + strand at 580.071 kb on - strand at 580.215 kb on - strand, within nohB at 580.215 kb on - strand, within nohB at 580.303 kb on - strand, within nohB at 580.605 kb on - strand at 580.642 kb on + strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.668 kb on - strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.733 kb on + strand, within ylcF at 580.944 kb on - strand, within tfaD at 580.973 kb on + strand, within tfaD at 580.981 kb on - strand, within tfaD at 580.981 kb on - strand, within tfaD at 581.152 kb on + strand, within tfaD at 581.171 kb on + strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.192 kb on - strand, within tfaD at 581.252 kb on + strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.260 kb on - strand, within tfaD at 581.262 kb on + strand, within tfaD at 581.269 kb on - strand, within tfaD at 581.285 kb on + strand at 581.307 kb on + strand at 581.307 kb on + strand at 581.343 kb on + strand at 581.344 kb on + strand at 581.344 kb on + strand at 581.352 kb on - strand at 581.404 kb on + strand at 581.411 kb on + strand at 581.443 kb on + strand, within ybcY at 581.450 kb on + strand, within ybcY at 581.453 kb on - strand, within ybcY at 581.460 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.524 kb on + strand, within ybcY at 581.572 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.574 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.576 kb on + strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.584 kb on - strand, within ybcY at 581.595 kb on + strand, within ybcY at 581.603 kb on - strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.654 kb on + strand, within ybcY at 581.665 kb on - strand, within ybcY at 581.672 kb on - strand, within ybcY at 581.701 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.704 kb on - strand, within ybcY at 581.717 kb on - strand, within ybcY at 581.766 kb on + strand, within ybcY at 581.772 kb on + strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.772 kb on - strand, within ybcY at 581.774 kb on - strand, within ybcY at 581.785 kb on + strand, within ybcY at 581.816 kb on - strand, within ybcY at 581.822 kb on + strand, within ybcY at 581.830 kb on - strand, within ybcY at 581.831 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.836 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on + strand, within ybcY at 581.841 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.862 kb on - strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.876 kb on + strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.877 kb on - strand, within ybcY at 581.884 kb on - strand, within ybcY
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 580,052 + -2.6 580,071 - +0.5 580,215 - nohB b0560 0.29 -1.6 580,215 - nohB b0560 0.29 +2.4 580,303 - nohB b0560 0.45 -0.2 580,605 - -0.1 580,642 + ylcF b4634 0.21 -0.4 580,668 - ylcF b4634 0.29 +0.2 580,668 - ylcF b4634 0.29 -1.6 580,733 + ylcF b4634 0.50 +2.2 580,733 + ylcF b4634 0.50 -0.2 580,733 + ylcF b4634 0.50 -0.7 580,944 - tfaD b0561 0.14 +1.0 580,973 + tfaD b0561 0.21 +1.9 580,981 - tfaD b0561 0.22 +1.2 580,981 - tfaD b0561 0.22 -0.9 581,152 + tfaD b0561 0.61 +0.9 581,171 + tfaD b0561 0.66 -0.8 581,192 - tfaD b0561 0.71 -2.2 581,192 - tfaD b0561 0.71 -0.4 581,252 + tfaD b0561 0.84 +2.3 581,260 - tfaD b0561 0.86 -1.8 581,260 - tfaD b0561 0.86 -0.5 581,262 + tfaD b0561 0.87 -0.5 581,269 - tfaD b0561 0.88 -3.6 581,285 + -0.8 581,307 + +2.5 581,307 + -0.4 581,343 + +3.2 581,344 + -0.0 581,344 + +2.3 581,352 - -0.8 581,404 + -2.8 581,411 + +0.2 581,443 + ybcY b0562 0.10 +0.5 581,450 + ybcY b0562 0.11 +0.8 581,453 - ybcY b0562 0.12 -0.5 581,460 + ybcY b0562 0.13 +0.7 581,524 + ybcY b0562 0.23 +0.8 581,524 + ybcY b0562 0.23 +1.2 581,572 + ybcY b0562 0.30 +0.2 581,574 + ybcY b0562 0.30 -0.9 581,574 + ybcY b0562 0.30 -1.6 581,576 + ybcY b0562 0.31 +1.4 581,576 + ybcY b0562 0.31 +0.7 581,584 - ybcY b0562 0.32 -0.8 581,584 - ybcY b0562 0.32 +1.8 581,595 + ybcY b0562 0.34 +0.1 581,603 - ybcY b0562 0.35 -1.1 581,654 + ybcY b0562 0.43 -0.7 581,654 + ybcY b0562 0.43 -1.4 581,665 - ybcY b0562 0.44 -0.8 581,672 - ybcY b0562 0.45 +0.1 581,701 - ybcY b0562 0.50 +1.0 581,704 - ybcY b0562 0.50 +2.0 581,704 - ybcY b0562 0.50 +0.4 581,704 - ybcY b0562 0.50 -0.6 581,717 - ybcY b0562 0.52 +1.7 581,766 + ybcY b0562 0.60 -1.9 581,772 + ybcY b0562 0.61 +0.7 581,772 - ybcY b0562 0.61 +0.2 581,772 - ybcY b0562 0.61 +0.2 581,774 - ybcY b0562 0.61 -1.4 581,785 + ybcY b0562 0.63 -0.9 581,816 - ybcY b0562 0.67 +0.2 581,822 + ybcY b0562 0.68 -0.7 581,830 - ybcY b0562 0.69 -1.1 581,831 + ybcY b0562 0.70 +0.4 581,836 + ybcY b0562 0.70 +0.4 581,836 + ybcY b0562 0.70 -1.4 581,841 + ybcY b0562 0.71 -1.6 581,841 + ybcY b0562 0.71 +1.7 581,841 - ybcY b0562 0.71 -0.9 581,862 - ybcY b0562 0.74 -0.4 581,862 - ybcY b0562 0.74 +1.7 581,876 + ybcY b0562 0.76 -0.6 581,876 + ybcY b0562 0.76 -1.4 581,877 - ybcY b0562 0.77 +2.4 581,877 - ybcY b0562 0.77 +1.1 581,884 - ybcY b0562 0.78 -1.5
Or see this region's nucleotide sequence