Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfQ and ytfR are separated by 139 nucleotides ytfR and ytfT are separated by 10 nucleotides
b4227: ytfQ - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 4,447,985 to 4,448,941
ytfQ
b4485: ytfR - predicted sugar transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 4,449,081 to 4,450,583
ytfR
b4230: ytfT - predicted sugar transporter subunit: membrane component of ABC superfamily (RefSeq), at 4,450,594 to 4,451,619
ytfT
Position (kb)
4449
4450
4451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4448.345 kb on + strand, within ytfQ at 4448.347 kb on + strand, within ytfQ at 4448.429 kb on + strand, within ytfQ at 4448.429 kb on + strand, within ytfQ at 4448.452 kb on + strand, within ytfQ at 4448.538 kb on + strand, within ytfQ at 4448.630 kb on + strand, within ytfQ at 4448.630 kb on + strand, within ytfQ at 4448.630 kb on + strand, within ytfQ at 4448.630 kb on + strand, within ytfQ at 4448.653 kb on + strand, within ytfQ at 4448.675 kb on + strand, within ytfQ at 4448.732 kb on + strand, within ytfQ at 4448.770 kb on - strand, within ytfQ at 4448.825 kb on + strand, within ytfQ at 4448.825 kb on + strand, within ytfQ at 4448.884 kb on + strand at 4448.888 kb on - strand at 4448.890 kb on + strand at 4449.060 kb on + strand at 4449.060 kb on + strand at 4449.062 kb on + strand at 4449.062 kb on + strand at 4449.069 kb on - strand at 4449.249 kb on + strand, within ytfR at 4449.249 kb on + strand, within ytfR at 4449.257 kb on - strand, within ytfR at 4449.593 kb on + strand, within ytfR at 4449.705 kb on + strand, within ytfR at 4449.718 kb on - strand, within ytfR at 4449.953 kb on + strand, within ytfR at 4449.953 kb on + strand, within ytfR at 4450.019 kb on + strand, within ytfR at 4450.080 kb on + strand, within ytfR at 4450.088 kb on - strand, within ytfR at 4450.167 kb on + strand, within ytfR at 4450.175 kb on - strand, within ytfR at 4450.267 kb on - strand, within ytfR at 4450.268 kb on - strand, within ytfR at 4450.406 kb on - strand, within ytfR at 4450.406 kb on - strand, within ytfR at 4450.451 kb on - strand at 4450.478 kb on + strand at 4450.478 kb on + strand at 4450.592 kb on + strand at 4450.666 kb on - strand at 4450.668 kb on - strand at 4450.675 kb on - strand at 4450.702 kb on - strand, within ytfT at 4450.708 kb on - strand, within ytfT at 4450.716 kb on - strand, within ytfT at 4450.755 kb on - strand, within ytfT at 4450.755 kb on - strand, within ytfT at 4450.760 kb on - strand, within ytfT at 4450.765 kb on - strand, within ytfT at 4450.765 kb on - strand, within ytfT at 4450.826 kb on - strand, within ytfT at 4450.832 kb on + strand, within ytfT at 4450.843 kb on - strand, within ytfT at 4450.853 kb on - strand, within ytfT at 4450.858 kb on + strand, within ytfT at 4450.865 kb on - strand, within ytfT at 4450.865 kb on - strand, within ytfT at 4451.166 kb on + strand, within ytfT at 4451.218 kb on + strand, within ytfT at 4451.218 kb on + strand, within ytfT at 4451.226 kb on - strand, within ytfT at 4451.287 kb on - strand, within ytfT at 4451.298 kb on + strand, within ytfT at 4451.302 kb on - strand, within ytfT at 4451.302 kb on - strand, within ytfT at 4451.360 kb on + strand, within ytfT at 4451.473 kb on + strand, within ytfT
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,448,345 + ytfQ b4227 0.38 -1.8 4,448,347 + ytfQ b4227 0.38 -1.6 4,448,429 + ytfQ b4227 0.46 +0.4 4,448,429 + ytfQ b4227 0.46 +1.4 4,448,452 + ytfQ b4227 0.49 +2.4 4,448,538 + ytfQ b4227 0.58 -0.9 4,448,630 + ytfQ b4227 0.67 -0.2 4,448,630 + ytfQ b4227 0.67 -1.4 4,448,630 + ytfQ b4227 0.67 +2.1 4,448,630 + ytfQ b4227 0.67 -0.9 4,448,653 + ytfQ b4227 0.70 -0.6 4,448,675 + ytfQ b4227 0.72 +1.4 4,448,732 + ytfQ b4227 0.78 +0.8 4,448,770 - ytfQ b4227 0.82 +2.5 4,448,825 + ytfQ b4227 0.88 +0.4 4,448,825 + ytfQ b4227 0.88 -1.6 4,448,884 + -1.8 4,448,888 - +0.5 4,448,890 + -0.2 4,449,060 + +0.8 4,449,060 + +0.4 4,449,062 + -0.2 4,449,062 + -0.8 4,449,069 - +0.4 4,449,249 + ytfR b4485 0.11 -2.7 4,449,249 + ytfR b4485 0.11 +0.2 4,449,257 - ytfR b4485 0.12 +1.8 4,449,593 + ytfR b4485 0.34 +0.1 4,449,705 + ytfR b4485 0.42 +0.4 4,449,718 - ytfR b4485 0.42 +0.2 4,449,953 + ytfR b4485 0.58 +0.4 4,449,953 + ytfR b4485 0.58 +1.5 4,450,019 + ytfR b4485 0.62 -0.9 4,450,080 + ytfR b4485 0.66 -1.6 4,450,088 - ytfR b4485 0.67 -0.6 4,450,167 + ytfR b4485 0.72 -1.9 4,450,175 - ytfR b4485 0.73 -2.5 4,450,267 - ytfR b4485 0.79 -0.3 4,450,268 - ytfR b4485 0.79 -1.6 4,450,406 - ytfR b4485 0.88 -0.0 4,450,406 - ytfR b4485 0.88 -0.8 4,450,451 - +2.3 4,450,478 + -0.2 4,450,478 + +1.1 4,450,592 + +0.3 4,450,666 - +0.2 4,450,668 - -0.4 4,450,675 - -1.1 4,450,702 - ytfT b4230 0.11 +0.4 4,450,708 - ytfT b4230 0.11 -2.1 4,450,716 - ytfT b4230 0.12 +1.0 4,450,755 - ytfT b4230 0.16 -0.6 4,450,755 - ytfT b4230 0.16 -1.9 4,450,760 - ytfT b4230 0.16 -0.2 4,450,765 - ytfT b4230 0.17 +0.2 4,450,765 - ytfT b4230 0.17 -1.1 4,450,826 - ytfT b4230 0.23 +0.3 4,450,832 + ytfT b4230 0.23 +1.0 4,450,843 - ytfT b4230 0.24 -0.2 4,450,853 - ytfT b4230 0.25 +1.5 4,450,858 + ytfT b4230 0.26 -1.3 4,450,865 - ytfT b4230 0.26 -0.2 4,450,865 - ytfT b4230 0.26 +0.8 4,451,166 + ytfT b4230 0.56 -0.2 4,451,218 + ytfT b4230 0.61 +1.6 4,451,218 + ytfT b4230 0.61 -1.2 4,451,226 - ytfT b4230 0.62 +0.1 4,451,287 - ytfT b4230 0.68 +0.6 4,451,298 + ytfT b4230 0.69 -3.3 4,451,302 - ytfT b4230 0.69 -0.4 4,451,302 - ytfT b4230 0.69 +0.4 4,451,360 + ytfT b4230 0.75 +0.5 4,451,473 + ytfT b4230 0.86 -0.2
Or see this region's nucleotide sequence