Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpiR and rpiB are separated by 358 nucleotides rpiB and yjdP are separated by 68 nucleotides yjdP and phnP are separated by 146 nucleotides phnP and phnO are separated by 1 nucleotides
b4089: rpiR - transcriptional repressor of rpiB expression (VIMSS), at 4,310,124 to 4,311,014
rpiR
b4090: rpiB - ribose-5-phosphate isomerase B (NCBI), at 4,311,373 to 4,311,822
rpiB
b4487: yjdP - hypothetical protein (NCBI), at 4,311,891 to 4,312,220
yjdP
b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125
phnP
b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561
phnO
Position (kb)
4311
4312
4313 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 4310.919 kb on - strand, within rpiR at 4310.919 kb on - strand, within rpiR at 4310.943 kb on - strand at 4310.969 kb on + strand at 4310.969 kb on + strand at 4310.969 kb on + strand at 4310.969 kb on + strand at 4310.971 kb on - strand at 4310.977 kb on - strand at 4310.977 kb on - strand at 4310.977 kb on - strand at 4310.977 kb on - strand at 4310.978 kb on - strand at 4310.992 kb on + strand at 4311.021 kb on + strand at 4311.052 kb on - strand at 4311.056 kb on - strand at 4311.242 kb on + strand at 4311.242 kb on + strand at 4311.499 kb on - strand, within rpiB at 4311.629 kb on + strand, within rpiB at 4311.639 kb on + strand, within rpiB at 4311.639 kb on + strand, within rpiB at 4311.674 kb on - strand, within rpiB at 4311.710 kb on + strand, within rpiB at 4311.744 kb on + strand, within rpiB at 4311.744 kb on + strand, within rpiB at 4311.744 kb on + strand, within rpiB at 4311.754 kb on + strand, within rpiB at 4311.756 kb on + strand, within rpiB at 4311.756 kb on + strand, within rpiB at 4311.756 kb on + strand, within rpiB at 4311.797 kb on + strand at 4311.852 kb on - strand at 4311.852 kb on - strand at 4311.889 kb on - strand at 4311.933 kb on - strand, within yjdP at 4311.933 kb on - strand, within yjdP at 4311.943 kb on - strand, within yjdP at 4311.969 kb on - strand, within yjdP at 4311.969 kb on - strand, within yjdP at 4311.983 kb on - strand, within yjdP at 4311.983 kb on - strand, within yjdP at 4312.006 kb on + strand, within yjdP at 4312.006 kb on + strand, within yjdP at 4312.006 kb on + strand, within yjdP at 4312.020 kb on - strand, within yjdP at 4312.068 kb on - strand, within yjdP at 4312.193 kb on + strand at 4312.202 kb on + strand at 4312.227 kb on - strand at 4312.256 kb on + strand at 4312.263 kb on - strand at 4312.338 kb on - strand at 4312.375 kb on + strand at 4312.418 kb on - strand at 4312.418 kb on - strand at 4312.567 kb on + strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.700 kb on + strand, within phnP at 4312.714 kb on - strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.721 kb on - strand, within phnP at 4312.728 kb on - strand, within phnP at 4312.788 kb on - strand, within phnP at 4312.803 kb on + strand, within phnP at 4312.808 kb on + strand, within phnP at 4312.948 kb on - strand, within phnP at 4312.965 kb on - strand, within phnP at 4313.024 kb on - strand, within phnP at 4313.041 kb on + strand, within phnP at 4313.060 kb on + strand at 4313.094 kb on - strand at 4313.115 kb on - strand at 4313.115 kb on - strand at 4313.127 kb on + strand at 4313.185 kb on - strand, within phnO at 4313.195 kb on - strand, within phnO
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,310,919 - rpiR b4089 0.89 +1.0 4,310,919 - rpiR b4089 0.89 +1.0 4,310,943 - +0.3 4,310,969 + -0.7 4,310,969 + +0.4 4,310,969 + -0.7 4,310,969 + -1.1 4,310,971 - +2.2 4,310,977 - +2.1 4,310,977 - +0.7 4,310,977 - -1.6 4,310,977 - +0.9 4,310,978 - +0.1 4,310,992 + +2.4 4,311,021 + -0.0 4,311,052 - +2.8 4,311,056 - +0.9 4,311,242 + -1.4 4,311,242 + +5.6 4,311,499 - rpiB b4090 0.28 +0.6 4,311,629 + rpiB b4090 0.57 -2.2 4,311,639 + rpiB b4090 0.59 -0.4 4,311,639 + rpiB b4090 0.59 +0.6 4,311,674 - rpiB b4090 0.67 -1.7 4,311,710 + rpiB b4090 0.75 -2.0 4,311,744 + rpiB b4090 0.82 +0.2 4,311,744 + rpiB b4090 0.82 +1.8 4,311,744 + rpiB b4090 0.82 -0.8 4,311,754 + rpiB b4090 0.85 +0.2 4,311,756 + rpiB b4090 0.85 -0.1 4,311,756 + rpiB b4090 0.85 -0.5 4,311,756 + rpiB b4090 0.85 -0.3 4,311,797 + -0.2 4,311,852 - +1.4 4,311,852 - +1.4 4,311,889 - -0.2 4,311,933 - yjdP b4487 0.13 -0.7 4,311,933 - yjdP b4487 0.13 -1.4 4,311,943 - yjdP b4487 0.16 +0.7 4,311,969 - yjdP b4487 0.24 -0.6 4,311,969 - yjdP b4487 0.24 -1.7 4,311,983 - yjdP b4487 0.28 -1.2 4,311,983 - yjdP b4487 0.28 -0.6 4,312,006 + yjdP b4487 0.35 -1.6 4,312,006 + yjdP b4487 0.35 -2.3 4,312,006 + yjdP b4487 0.35 -0.7 4,312,020 - yjdP b4487 0.39 -0.2 4,312,068 - yjdP b4487 0.54 -1.1 4,312,193 + -0.2 4,312,202 + -2.9 4,312,227 - +3.0 4,312,256 + +1.3 4,312,263 - +0.7 4,312,338 - -0.2 4,312,375 + +0.3 4,312,418 - -1.0 4,312,418 - -1.4 4,312,567 + phnP b4092 0.26 +5.8 4,312,658 - phnP b4092 0.38 +0.4 4,312,658 - phnP b4092 0.38 -1.4 4,312,663 - phnP b4092 0.39 +1.4 4,312,663 - phnP b4092 0.39 -1.7 4,312,668 - phnP b4092 0.40 +0.8 4,312,668 - phnP b4092 0.40 +0.9 4,312,700 + phnP b4092 0.44 +0.9 4,312,714 - phnP b4092 0.46 -0.0 4,312,720 + phnP b4092 0.47 +0.4 4,312,720 + phnP b4092 0.47 +0.4 4,312,721 - phnP b4092 0.47 +0.8 4,312,728 - phnP b4092 0.48 -1.8 4,312,788 - phnP b4092 0.55 +1.0 4,312,803 + phnP b4092 0.57 +0.4 4,312,808 + phnP b4092 0.58 -1.2 4,312,948 - phnP b4092 0.77 -0.3 4,312,965 - phnP b4092 0.79 -1.1 4,313,024 - phnP b4092 0.87 -0.3 4,313,041 + phnP b4092 0.89 -2.0 4,313,060 + +0.7 4,313,094 - -0.9 4,313,115 - -1.6 4,313,115 - -0.9 4,313,127 + -0.9 4,313,185 - phnO b4093 0.13 -0.4 4,313,195 - phnO b4093 0.16 +0.7
Or see this region's nucleotide sequence