Strain Fitness in Escherichia coli BW25113 around b4487

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrpiR and rpiB are separated by 358 nucleotidesrpiB and yjdP are separated by 68 nucleotidesyjdP and phnP are separated by 146 nucleotidesphnP and phnO are separated by 1 nucleotides b4089: rpiR - transcriptional repressor of rpiB expression (VIMSS), at 4,310,124 to 4,311,014 rpiR b4090: rpiB - ribose-5-phosphate isomerase B (NCBI), at 4,311,373 to 4,311,822 rpiB b4487: yjdP - hypothetical protein (NCBI), at 4,311,891 to 4,312,220 yjdP b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125 phnP b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561 phnO Position (kb) 4311 4312 4313Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5 6at 4310.919 kb on - strand, within rpiRat 4310.919 kb on - strand, within rpiRat 4310.943 kb on - strandat 4310.969 kb on + strandat 4310.969 kb on + strandat 4310.969 kb on + strandat 4310.969 kb on + strandat 4310.971 kb on - strandat 4310.977 kb on - strandat 4310.977 kb on - strandat 4310.977 kb on - strandat 4310.977 kb on - strandat 4310.978 kb on - strandat 4310.992 kb on + strandat 4311.021 kb on + strandat 4311.052 kb on - strandat 4311.056 kb on - strandat 4311.242 kb on + strandat 4311.242 kb on + strandat 4311.499 kb on - strand, within rpiBat 4311.629 kb on + strand, within rpiBat 4311.639 kb on + strand, within rpiBat 4311.639 kb on + strand, within rpiBat 4311.674 kb on - strand, within rpiBat 4311.710 kb on + strand, within rpiBat 4311.744 kb on + strand, within rpiBat 4311.744 kb on + strand, within rpiBat 4311.744 kb on + strand, within rpiBat 4311.754 kb on + strand, within rpiBat 4311.756 kb on + strand, within rpiBat 4311.756 kb on + strand, within rpiBat 4311.756 kb on + strand, within rpiBat 4311.797 kb on + strandat 4311.852 kb on - strandat 4311.852 kb on - strandat 4311.889 kb on - strandat 4311.933 kb on - strand, within yjdPat 4311.933 kb on - strand, within yjdPat 4311.943 kb on - strand, within yjdPat 4311.969 kb on - strand, within yjdPat 4311.969 kb on - strand, within yjdPat 4311.983 kb on - strand, within yjdPat 4311.983 kb on - strand, within yjdPat 4312.006 kb on + strand, within yjdPat 4312.006 kb on + strand, within yjdPat 4312.006 kb on + strand, within yjdPat 4312.020 kb on - strand, within yjdPat 4312.068 kb on - strand, within yjdPat 4312.193 kb on + strandat 4312.202 kb on + strandat 4312.227 kb on - strandat 4312.256 kb on + strandat 4312.263 kb on - strandat 4312.338 kb on - strandat 4312.375 kb on + strandat 4312.418 kb on - strandat 4312.418 kb on - strandat 4312.567 kb on + strand, within phnPat 4312.658 kb on - strand, within phnPat 4312.658 kb on - strand, within phnPat 4312.663 kb on - strand, within phnPat 4312.663 kb on - strand, within phnPat 4312.668 kb on - strand, within phnPat 4312.668 kb on - strand, within phnPat 4312.700 kb on + strand, within phnPat 4312.714 kb on - strand, within phnPat 4312.720 kb on + strand, within phnPat 4312.720 kb on + strand, within phnPat 4312.721 kb on - strand, within phnPat 4312.728 kb on - strand, within phnPat 4312.788 kb on - strand, within phnPat 4312.803 kb on + strand, within phnPat 4312.808 kb on + strand, within phnPat 4312.948 kb on - strand, within phnPat 4312.965 kb on - strand, within phnPat 4313.024 kb on - strand, within phnPat 4313.041 kb on + strand, within phnPat 4313.060 kb on + strandat 4313.094 kb on - strandat 4313.115 kb on - strandat 4313.115 kb on - strandat 4313.127 kb on + strandat 4313.185 kb on - strand, within phnOat 4313.195 kb on - strand, within phnO

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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4,310,919 - rpiR b4089 0.89 +1.0
4,310,919 - rpiR b4089 0.89 +1.0
4,310,943 - +0.3
4,310,969 + -0.7
4,310,969 + +0.4
4,310,969 + -0.7
4,310,969 + -1.1
4,310,971 - +2.2
4,310,977 - +2.1
4,310,977 - +0.7
4,310,977 - -1.6
4,310,977 - +0.9
4,310,978 - +0.1
4,310,992 + +2.4
4,311,021 + -0.0
4,311,052 - +2.8
4,311,056 - +0.9
4,311,242 + -1.4
4,311,242 + +5.6
4,311,499 - rpiB b4090 0.28 +0.6
4,311,629 + rpiB b4090 0.57 -2.2
4,311,639 + rpiB b4090 0.59 -0.4
4,311,639 + rpiB b4090 0.59 +0.6
4,311,674 - rpiB b4090 0.67 -1.7
4,311,710 + rpiB b4090 0.75 -2.0
4,311,744 + rpiB b4090 0.82 +0.2
4,311,744 + rpiB b4090 0.82 +1.8
4,311,744 + rpiB b4090 0.82 -0.8
4,311,754 + rpiB b4090 0.85 +0.2
4,311,756 + rpiB b4090 0.85 -0.1
4,311,756 + rpiB b4090 0.85 -0.5
4,311,756 + rpiB b4090 0.85 -0.3
4,311,797 + -0.2
4,311,852 - +1.4
4,311,852 - +1.4
4,311,889 - -0.2
4,311,933 - yjdP b4487 0.13 -0.7
4,311,933 - yjdP b4487 0.13 -1.4
4,311,943 - yjdP b4487 0.16 +0.7
4,311,969 - yjdP b4487 0.24 -0.6
4,311,969 - yjdP b4487 0.24 -1.7
4,311,983 - yjdP b4487 0.28 -1.2
4,311,983 - yjdP b4487 0.28 -0.6
4,312,006 + yjdP b4487 0.35 -1.6
4,312,006 + yjdP b4487 0.35 -2.3
4,312,006 + yjdP b4487 0.35 -0.7
4,312,020 - yjdP b4487 0.39 -0.2
4,312,068 - yjdP b4487 0.54 -1.1
4,312,193 + -0.2
4,312,202 + -2.9
4,312,227 - +3.0
4,312,256 + +1.3
4,312,263 - +0.7
4,312,338 - -0.2
4,312,375 + +0.3
4,312,418 - -1.0
4,312,418 - -1.4
4,312,567 + phnP b4092 0.26 +5.8
4,312,658 - phnP b4092 0.38 +0.4
4,312,658 - phnP b4092 0.38 -1.4
4,312,663 - phnP b4092 0.39 +1.4
4,312,663 - phnP b4092 0.39 -1.7
4,312,668 - phnP b4092 0.40 +0.8
4,312,668 - phnP b4092 0.40 +0.9
4,312,700 + phnP b4092 0.44 +0.9
4,312,714 - phnP b4092 0.46 -0.0
4,312,720 + phnP b4092 0.47 +0.4
4,312,720 + phnP b4092 0.47 +0.4
4,312,721 - phnP b4092 0.47 +0.8
4,312,728 - phnP b4092 0.48 -1.8
4,312,788 - phnP b4092 0.55 +1.0
4,312,803 + phnP b4092 0.57 +0.4
4,312,808 + phnP b4092 0.58 -1.2
4,312,948 - phnP b4092 0.77 -0.3
4,312,965 - phnP b4092 0.79 -1.1
4,313,024 - phnP b4092 0.87 -0.3
4,313,041 + phnP b4092 0.89 -2.0
4,313,060 + +0.7
4,313,094 - -0.9
4,313,115 - -1.6
4,313,115 - -0.9
4,313,127 + -0.9
4,313,185 - phnO b4093 0.13 -0.4
4,313,195 - phnO b4093 0.16 +0.7

Or see this region's nucleotide sequence