Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tatB and tatC are separated by 2 nucleotides tatC and tatD are separated by 41 nucleotides tatD and rfaH overlap by 4 nucleotides rfaH and ubiD are separated by 166 nucleotides
b3838: tatB - sec-independent translocase (NCBI), at 4,020,241 to 4,020,756
tatB
b3839: tatC - TatABCE protein translocation system subunit (NCBI), at 4,020,759 to 4,021,535
tatC
b4483: tatD - DNase, magnesium-dependent (NCBI), at 4,021,577 to 4,022,359
tatD
b3842: rfaH - transcriptional activator RfaH (NCBI), at 4,022,356 to 4,022,844
rfaH
b3843: ubiD - 3-octaprenyl-4-hydroxybenzoate decarboxylase (NCBI), at 4,023,011 to 4,024,504
ubiD
Position (kb)
4021
4022
4023 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 4020.579 kb on - strand, within tatB at 4020.596 kb on + strand, within tatB at 4020.640 kb on - strand, within tatB at 4020.645 kb on - strand, within tatB at 4020.686 kb on + strand, within tatB at 4020.727 kb on - strand at 4020.750 kb on + strand at 4020.771 kb on - strand at 4020.777 kb on - strand at 4020.782 kb on + strand at 4020.796 kb on + strand at 4020.804 kb on - strand at 4020.814 kb on - strand at 4020.814 kb on - strand at 4020.814 kb on - strand at 4020.824 kb on - strand at 4020.839 kb on + strand, within tatC at 4020.845 kb on + strand, within tatC at 4020.851 kb on - strand, within tatC at 4020.881 kb on + strand, within tatC at 4020.891 kb on + strand, within tatC at 4020.891 kb on + strand, within tatC at 4020.891 kb on + strand, within tatC at 4020.891 kb on - strand, within tatC at 4020.902 kb on - strand, within tatC at 4020.910 kb on + strand, within tatC at 4020.910 kb on + strand, within tatC at 4020.933 kb on - strand, within tatC at 4020.990 kb on - strand, within tatC at 4021.018 kb on + strand, within tatC at 4021.028 kb on + strand, within tatC at 4021.028 kb on + strand, within tatC at 4021.030 kb on - strand, within tatC at 4021.030 kb on - strand, within tatC at 4021.045 kb on + strand, within tatC at 4021.053 kb on + strand, within tatC at 4021.053 kb on + strand, within tatC at 4021.053 kb on + strand, within tatC at 4021.055 kb on + strand, within tatC at 4021.055 kb on + strand, within tatC at 4021.055 kb on + strand, within tatC at 4021.063 kb on - strand, within tatC at 4021.063 kb on - strand, within tatC at 4021.063 kb on - strand, within tatC at 4021.063 kb on - strand, within tatC at 4021.070 kb on - strand, within tatC at 4021.078 kb on - strand, within tatC at 4021.109 kb on - strand, within tatC at 4021.167 kb on + strand, within tatC at 4021.167 kb on + strand, within tatC at 4021.169 kb on + strand, within tatC at 4021.169 kb on + strand, within tatC at 4021.177 kb on - strand, within tatC at 4021.205 kb on - strand, within tatC at 4021.228 kb on + strand, within tatC at 4021.228 kb on + strand, within tatC at 4021.241 kb on + strand, within tatC at 4021.241 kb on + strand, within tatC at 4021.261 kb on + strand, within tatC at 4021.269 kb on - strand, within tatC at 4021.269 kb on - strand, within tatC at 4021.269 kb on - strand, within tatC at 4021.279 kb on - strand, within tatC at 4021.305 kb on - strand, within tatC at 4021.305 kb on - strand, within tatC at 4021.344 kb on + strand, within tatC at 4021.418 kb on + strand, within tatC at 4021.419 kb on + strand, within tatC at 4021.443 kb on + strand, within tatC at 4021.470 kb on + strand at 4021.489 kb on - strand at 4021.555 kb on - strand at 4021.555 kb on - strand at 4021.568 kb on + strand at 4021.568 kb on + strand at 4021.570 kb on + strand at 4021.647 kb on + strand at 4021.666 kb on - strand at 4021.666 kb on - strand, within tatD at 4021.667 kb on + strand, within tatD at 4021.670 kb on - strand, within tatD at 4021.670 kb on - strand, within tatD at 4021.680 kb on - strand, within tatD at 4021.683 kb on + strand, within tatD at 4021.683 kb on + strand, within tatD at 4021.683 kb on + strand, within tatD at 4021.683 kb on + strand, within tatD at 4021.691 kb on - strand, within tatD at 4021.691 kb on - strand, within tatD at 4021.691 kb on - strand, within tatD at 4021.718 kb on - strand, within tatD at 4021.718 kb on - strand, within tatD at 4021.733 kb on - strand, within tatD at 4021.733 kb on - strand, within tatD at 4021.768 kb on + strand, within tatD at 4021.768 kb on + strand, within tatD at 4021.778 kb on - strand, within tatD at 4021.783 kb on - strand, within tatD at 4021.825 kb on + strand, within tatD at 4021.825 kb on + strand, within tatD at 4021.923 kb on - strand, within tatD at 4021.931 kb on + strand, within tatD at 4021.931 kb on + strand, within tatD at 4021.959 kb on + strand, within tatD at 4021.959 kb on + strand, within tatD at 4021.978 kb on + strand, within tatD at 4021.978 kb on + strand, within tatD at 4021.978 kb on + strand, within tatD at 4021.986 kb on - strand, within tatD at 4021.986 kb on - strand, within tatD at 4021.986 kb on - strand, within tatD at 4021.986 kb on - strand, within tatD at 4021.994 kb on - strand, within tatD at 4022.055 kb on + strand, within tatD at 4022.055 kb on + strand, within tatD at 4022.056 kb on - strand, within tatD at 4022.124 kb on + strand, within tatD at 4022.191 kb on + strand, within tatD at 4022.223 kb on + strand, within tatD at 4022.252 kb on - strand, within tatD at 4022.261 kb on + strand, within tatD at 4022.271 kb on + strand, within tatD at 4022.271 kb on + strand, within tatD at 4022.271 kb on + strand, within tatD at 4022.271 kb on + strand, within tatD at 4022.284 kb on + strand at 4022.284 kb on + strand at 4022.284 kb on + strand at 4022.284 kb on - strand at 4022.293 kb on + strand at 4022.294 kb on + strand at 4022.306 kb on - strand at 4022.306 kb on - strand at 4022.327 kb on - strand at 4022.339 kb on - strand at 4022.353 kb on + strand at 4022.353 kb on + strand at 4022.376 kb on - strand at 4022.376 kb on - strand at 4022.379 kb on + strand at 4022.379 kb on + strand at 4022.387 kb on - strand at 4022.389 kb on - strand at 4022.399 kb on - strand at 4022.424 kb on + strand, within rfaH at 4022.424 kb on + strand, within rfaH at 4022.431 kb on - strand, within rfaH at 4022.434 kb on + strand, within rfaH at 4022.437 kb on - strand, within rfaH at 4022.495 kb on - strand, within rfaH at 4022.500 kb on - strand, within rfaH at 4022.500 kb on - strand, within rfaH at 4022.557 kb on + strand, within rfaH at 4022.559 kb on + strand, within rfaH at 4022.563 kb on + strand, within rfaH at 4022.585 kb on + strand, within rfaH at 4022.624 kb on - strand, within rfaH at 4022.634 kb on + strand, within rfaH at 4022.650 kb on - strand, within rfaH at 4022.688 kb on - strand, within rfaH at 4022.701 kb on + strand, within rfaH at 4022.701 kb on + strand, within rfaH at 4022.709 kb on + strand, within rfaH at 4022.759 kb on + strand, within rfaH at 4022.762 kb on - strand, within rfaH at 4022.764 kb on + strand, within rfaH at 4022.765 kb on + strand, within rfaH at 4022.777 kb on + strand, within rfaH at 4022.793 kb on - strand, within rfaH at 4022.813 kb on + strand at 4022.816 kb on + strand at 4022.824 kb on + strand at 4022.849 kb on + strand at 4022.857 kb on - strand at 4022.884 kb on - strand at 4022.905 kb on - strand at 4022.920 kb on + strand at 4022.980 kb on + strand at 4022.980 kb on + strand at 4022.986 kb on + strand at 4022.996 kb on + strand at 4022.996 kb on + strand at 4022.996 kb on + strand at 4022.996 kb on + strand at 4023.004 kb on - strand at 4023.004 kb on - strand at 4023.037 kb on + strand at 4023.037 kb on + strand at 4023.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,020,579 - tatB b3838 0.66 +0.3 4,020,596 + tatB b3838 0.69 -0.8 4,020,640 - tatB b3838 0.77 -2.3 4,020,645 - tatB b3838 0.78 +0.5 4,020,686 + tatB b3838 0.86 +1.8 4,020,727 - +1.3 4,020,750 + +0.9 4,020,771 - -0.5 4,020,777 - -0.0 4,020,782 + +0.1 4,020,796 + +1.5 4,020,804 - -0.5 4,020,814 - -1.1 4,020,814 - -1.1 4,020,814 - -1.8 4,020,824 - -0.6 4,020,839 + tatC b3839 0.10 -1.3 4,020,845 + tatC b3839 0.11 -2.8 4,020,851 - tatC b3839 0.12 -2.3 4,020,881 + tatC b3839 0.16 +0.9 4,020,891 + tatC b3839 0.17 -1.3 4,020,891 + tatC b3839 0.17 +1.5 4,020,891 + tatC b3839 0.17 +0.5 4,020,891 - tatC b3839 0.17 -0.1 4,020,902 - tatC b3839 0.18 +0.4 4,020,910 + tatC b3839 0.19 -1.5 4,020,910 + tatC b3839 0.19 -0.6 4,020,933 - tatC b3839 0.22 -1.8 4,020,990 - tatC b3839 0.30 -1.0 4,021,018 + tatC b3839 0.33 -1.1 4,021,028 + tatC b3839 0.35 -0.4 4,021,028 + tatC b3839 0.35 -0.9 4,021,030 - tatC b3839 0.35 -0.1 4,021,030 - tatC b3839 0.35 +1.0 4,021,045 + tatC b3839 0.37 -0.8 4,021,053 + tatC b3839 0.38 -0.9 4,021,053 + tatC b3839 0.38 -0.9 4,021,053 + tatC b3839 0.38 -2.1 4,021,055 + tatC b3839 0.38 -1.9 4,021,055 + tatC b3839 0.38 -0.8 4,021,055 + tatC b3839 0.38 +1.1 4,021,063 - tatC b3839 0.39 -1.5 4,021,063 - tatC b3839 0.39 -0.1 4,021,063 - tatC b3839 0.39 -0.6 4,021,063 - tatC b3839 0.39 -0.5 4,021,070 - tatC b3839 0.40 -2.4 4,021,078 - tatC b3839 0.41 -1.8 4,021,109 - tatC b3839 0.45 -0.1 4,021,167 + tatC b3839 0.53 -0.1 4,021,167 + tatC b3839 0.53 -0.1 4,021,169 + tatC b3839 0.53 -0.2 4,021,169 + tatC b3839 0.53 -1.5 4,021,177 - tatC b3839 0.54 +0.5 4,021,205 - tatC b3839 0.57 -0.1 4,021,228 + tatC b3839 0.60 +0.8 4,021,228 + tatC b3839 0.60 -0.5 4,021,241 + tatC b3839 0.62 +1.5 4,021,241 + tatC b3839 0.62 +0.5 4,021,261 + tatC b3839 0.65 -2.7 4,021,269 - tatC b3839 0.66 -1.8 4,021,269 - tatC b3839 0.66 -1.3 4,021,269 - tatC b3839 0.66 -0.5 4,021,279 - tatC b3839 0.67 -1.5 4,021,305 - tatC b3839 0.70 -1.6 4,021,305 - tatC b3839 0.70 -0.6 4,021,344 + tatC b3839 0.75 -2.0 4,021,418 + tatC b3839 0.85 -1.7 4,021,419 + tatC b3839 0.85 -1.1 4,021,443 + tatC b3839 0.88 -1.0 4,021,470 + +0.8 4,021,489 - -0.5 4,021,555 - +2.2 4,021,555 - -0.6 4,021,568 + -1.5 4,021,568 + -0.5 4,021,570 + +0.3 4,021,647 + -0.4 4,021,666 - +1.5 4,021,666 - tatD b4483 0.11 -0.9 4,021,667 + tatD b4483 0.11 +0.2 4,021,670 - tatD b4483 0.12 -0.8 4,021,670 - tatD b4483 0.12 -0.9 4,021,680 - tatD b4483 0.13 +0.4 4,021,683 + tatD b4483 0.14 +0.2 4,021,683 + tatD b4483 0.14 +4.6 4,021,683 + tatD b4483 0.14 -0.1 4,021,683 + tatD b4483 0.14 +0.1 4,021,691 - tatD b4483 0.15 -0.1 4,021,691 - tatD b4483 0.15 -1.5 4,021,691 - tatD b4483 0.15 +0.2 4,021,718 - tatD b4483 0.18 +1.5 4,021,718 - tatD b4483 0.18 +0.3 4,021,733 - tatD b4483 0.20 +0.1 4,021,733 - tatD b4483 0.20 -0.6 4,021,768 + tatD b4483 0.24 -0.1 4,021,768 + tatD b4483 0.24 -0.1 4,021,778 - tatD b4483 0.26 -0.5 4,021,783 - tatD b4483 0.26 +0.8 4,021,825 + tatD b4483 0.32 +0.5 4,021,825 + tatD b4483 0.32 -0.3 4,021,923 - tatD b4483 0.44 -0.1 4,021,931 + tatD b4483 0.45 +0.9 4,021,931 + tatD b4483 0.45 -0.9 4,021,959 + tatD b4483 0.49 -2.1 4,021,959 + tatD b4483 0.49 +0.5 4,021,978 + tatD b4483 0.51 +1.2 4,021,978 + tatD b4483 0.51 -0.9 4,021,978 + tatD b4483 0.51 +0.5 4,021,986 - tatD b4483 0.52 -0.7 4,021,986 - tatD b4483 0.52 -0.2 4,021,986 - tatD b4483 0.52 -1.2 4,021,986 - tatD b4483 0.52 -0.9 4,021,994 - tatD b4483 0.53 -2.3 4,022,055 + tatD b4483 0.61 +0.5 4,022,055 + tatD b4483 0.61 +0.4 4,022,056 - tatD b4483 0.61 -1.3 4,022,124 + tatD b4483 0.70 +0.0 4,022,191 + tatD b4483 0.78 -0.9 4,022,223 + tatD b4483 0.83 -0.6 4,022,252 - tatD b4483 0.86 +0.1 4,022,261 + tatD b4483 0.87 -0.1 4,022,271 + tatD b4483 0.89 +1.5 4,022,271 + tatD b4483 0.89 -1.1 4,022,271 + tatD b4483 0.89 +0.2 4,022,271 + tatD b4483 0.89 +0.1 4,022,284 + -0.4 4,022,284 + -0.9 4,022,284 + +1.8 4,022,284 - -2.1 4,022,293 + +0.7 4,022,294 + +1.0 4,022,306 - -1.1 4,022,306 - -1.6 4,022,327 - +0.4 4,022,339 - +1.2 4,022,353 + -1.4 4,022,353 + -2.6 4,022,376 - +1.1 4,022,376 - -2.2 4,022,379 + +2.3 4,022,379 + -0.1 4,022,387 - -0.3 4,022,389 - +0.9 4,022,399 - -3.5 4,022,424 + rfaH b3842 0.14 -0.7 4,022,424 + rfaH b3842 0.14 +0.7 4,022,431 - rfaH b3842 0.15 +0.9 4,022,434 + rfaH b3842 0.16 -0.5 4,022,437 - rfaH b3842 0.17 -0.1 4,022,495 - rfaH b3842 0.28 +1.5 4,022,500 - rfaH b3842 0.29 -0.1 4,022,500 - rfaH b3842 0.29 +0.8 4,022,557 + rfaH b3842 0.41 +0.5 4,022,559 + rfaH b3842 0.42 -0.1 4,022,563 + rfaH b3842 0.42 +1.1 4,022,585 + rfaH b3842 0.47 +0.5 4,022,624 - rfaH b3842 0.55 +0.5 4,022,634 + rfaH b3842 0.57 +2.5 4,022,650 - rfaH b3842 0.60 -0.1 4,022,688 - rfaH b3842 0.68 +0.3 4,022,701 + rfaH b3842 0.71 +0.5 4,022,701 + rfaH b3842 0.71 +0.8 4,022,709 + rfaH b3842 0.72 -0.5 4,022,759 + rfaH b3842 0.82 -0.8 4,022,762 - rfaH b3842 0.83 +0.1 4,022,764 + rfaH b3842 0.83 -1.3 4,022,765 + rfaH b3842 0.84 -1.2 4,022,777 + rfaH b3842 0.86 -0.5 4,022,793 - rfaH b3842 0.89 +1.5 4,022,813 + -0.1 4,022,816 + +2.3 4,022,824 + -0.8 4,022,849 + -1.3 4,022,857 - -1.3 4,022,884 - -0.6 4,022,905 - -0.8 4,022,920 + +0.8 4,022,980 + -0.5 4,022,980 + -2.5 4,022,986 + -0.5 4,022,996 + -0.3 4,022,996 + +0.7 4,022,996 + -0.5 4,022,996 + +0.4 4,023,004 - +0.1 4,023,004 - +1.3 4,023,037 + -0.5 4,023,037 + +1.1 4,023,043 - +5.4
Or see this region's nucleotide sequence