Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pldB and yigL are separated by 7 nucleotides yigL and yigM are separated by 75 nucleotides yigM and metR overlap by 113 nucleotides
b3825: pldB - lysophospholipase L(2) (NCBI), at 4,007,193 to 4,008,215
pldB
b3826: yigL - predicted hydrolase (NCBI), at 4,008,223 to 4,009,023
yigL
b3827: yigM - predicted inner membrane protein (NCBI), at 4,009,099 to 4,009,998
yigM
b3828: metR - DNA-binding transcriptional activator, homocysteine-binding (NCBI), at 4,009,886 to 4,010,839
metR
Position (kb)
4008
4009
4010 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4007.264 kb on - strand at 4007.264 kb on - strand at 4007.289 kb on - strand at 4007.289 kb on - strand at 4007.319 kb on - strand, within pldB at 4007.345 kb on + strand, within pldB at 4007.345 kb on + strand, within pldB at 4007.354 kb on - strand, within pldB at 4007.357 kb on + strand, within pldB at 4007.357 kb on + strand, within pldB at 4007.361 kb on + strand, within pldB at 4007.364 kb on - strand, within pldB at 4007.366 kb on + strand, within pldB at 4007.379 kb on - strand, within pldB at 4007.380 kb on + strand, within pldB at 4007.382 kb on + strand, within pldB at 4007.411 kb on + strand, within pldB at 4007.421 kb on - strand, within pldB at 4007.421 kb on - strand, within pldB at 4007.483 kb on + strand, within pldB at 4007.483 kb on + strand, within pldB at 4007.491 kb on - strand, within pldB at 4007.518 kb on - strand, within pldB at 4007.526 kb on - strand, within pldB at 4007.559 kb on + strand, within pldB at 4007.559 kb on + strand, within pldB at 4007.564 kb on + strand, within pldB at 4007.565 kb on + strand, within pldB at 4007.590 kb on + strand, within pldB at 4007.614 kb on - strand, within pldB at 4007.652 kb on + strand, within pldB at 4007.667 kb on + strand, within pldB at 4007.667 kb on + strand, within pldB at 4007.731 kb on + strand, within pldB at 4007.731 kb on + strand, within pldB at 4007.749 kb on - strand, within pldB at 4007.749 kb on - strand, within pldB at 4007.811 kb on + strand, within pldB at 4007.811 kb on + strand, within pldB at 4007.861 kb on + strand, within pldB at 4007.869 kb on - strand, within pldB at 4007.869 kb on - strand, within pldB at 4007.871 kb on - strand, within pldB at 4007.875 kb on + strand, within pldB at 4007.875 kb on + strand, within pldB at 4007.881 kb on - strand, within pldB at 4007.903 kb on - strand, within pldB at 4008.002 kb on - strand, within pldB at 4008.002 kb on - strand, within pldB at 4008.017 kb on + strand, within pldB at 4008.017 kb on - strand, within pldB at 4008.025 kb on - strand, within pldB at 4008.041 kb on + strand, within pldB at 4008.054 kb on - strand, within pldB at 4008.060 kb on - strand, within pldB at 4008.066 kb on + strand, within pldB at 4008.086 kb on + strand, within pldB at 4008.086 kb on + strand, within pldB at 4008.225 kb on + strand at 4008.230 kb on + strand at 4008.230 kb on + strand at 4008.302 kb on + strand at 4008.302 kb on + strand at 4008.310 kb on - strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.350 kb on + strand, within yigL at 4008.393 kb on - strand, within yigL at 4008.416 kb on + strand, within yigL at 4008.430 kb on + strand, within yigL at 4008.489 kb on - strand, within yigL at 4008.495 kb on + strand, within yigL at 4008.496 kb on - strand, within yigL at 4008.505 kb on + strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.526 kb on - strand, within yigL at 4008.529 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.572 kb on + strand, within yigL at 4008.621 kb on + strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.629 kb on - strand, within yigL at 4008.632 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.677 kb on + strand, within yigL at 4008.680 kb on + strand, within yigL at 4008.688 kb on - strand, within yigL at 4008.708 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.754 kb on + strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.762 kb on - strand, within yigL at 4008.903 kb on - strand, within yigL at 4008.910 kb on + strand, within yigL at 4008.918 kb on - strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.920 kb on + strand, within yigL at 4008.968 kb on + strand at 4009.067 kb on - strand at 4009.082 kb on - strand at 4009.134 kb on - strand at 4009.176 kb on - strand at 4009.176 kb on - strand at 4009.205 kb on - strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.275 kb on + strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.283 kb on - strand, within yigM at 4009.311 kb on - strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.334 kb on + strand, within yigM at 4009.338 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.348 kb on - strand, within yigM at 4009.406 kb on - strand, within yigM at 4009.428 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.434 kb on + strand, within yigM at 4009.451 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.495 kb on - strand, within yigM at 4009.506 kb on + strand, within yigM at 4009.524 kb on - strand, within yigM at 4009.600 kb on + strand, within yigM at 4009.772 kb on + strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.839 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.845 kb on - strand, within yigM at 4009.874 kb on + strand, within yigM at 4009.904 kb on + strand at 4009.917 kb on - strand at 4009.921 kb on - strand at 4009.921 kb on - strand at 4009.947 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,007,264 - +2.0 4,007,264 - -0.5 4,007,289 - +1.1 4,007,289 - -1.4 4,007,319 - pldB b3825 0.12 -0.2 4,007,345 + pldB b3825 0.15 -1.6 4,007,345 + pldB b3825 0.15 -0.1 4,007,354 - pldB b3825 0.16 +1.3 4,007,357 + pldB b3825 0.16 +3.3 4,007,357 + pldB b3825 0.16 +0.6 4,007,361 + pldB b3825 0.16 +1.0 4,007,364 - pldB b3825 0.17 -0.8 4,007,366 + pldB b3825 0.17 +0.5 4,007,379 - pldB b3825 0.18 -0.4 4,007,380 + pldB b3825 0.18 +3.7 4,007,382 + pldB b3825 0.18 -0.8 4,007,411 + pldB b3825 0.21 +0.5 4,007,421 - pldB b3825 0.22 +0.1 4,007,421 - pldB b3825 0.22 +1.7 4,007,483 + pldB b3825 0.28 -1.8 4,007,483 + pldB b3825 0.28 +2.9 4,007,491 - pldB b3825 0.29 +0.8 4,007,518 - pldB b3825 0.32 -0.1 4,007,526 - pldB b3825 0.33 -2.9 4,007,559 + pldB b3825 0.36 +0.5 4,007,559 + pldB b3825 0.36 -0.5 4,007,564 + pldB b3825 0.36 +0.9 4,007,565 + pldB b3825 0.36 +0.7 4,007,590 + pldB b3825 0.39 +0.9 4,007,614 - pldB b3825 0.41 +1.2 4,007,652 + pldB b3825 0.45 -0.6 4,007,667 + pldB b3825 0.46 +0.9 4,007,667 + pldB b3825 0.46 -0.8 4,007,731 + pldB b3825 0.53 +2.2 4,007,731 + pldB b3825 0.53 +1.4 4,007,749 - pldB b3825 0.54 -1.5 4,007,749 - pldB b3825 0.54 -0.5 4,007,811 + pldB b3825 0.60 -1.2 4,007,811 + pldB b3825 0.60 +2.3 4,007,861 + pldB b3825 0.65 +0.2 4,007,869 - pldB b3825 0.66 -2.3 4,007,869 - pldB b3825 0.66 +1.1 4,007,871 - pldB b3825 0.66 -1.9 4,007,875 + pldB b3825 0.67 -1.5 4,007,875 + pldB b3825 0.67 -0.3 4,007,881 - pldB b3825 0.67 +1.5 4,007,903 - pldB b3825 0.69 +0.8 4,008,002 - pldB b3825 0.79 -0.1 4,008,002 - pldB b3825 0.79 +0.2 4,008,017 + pldB b3825 0.81 -1.8 4,008,017 - pldB b3825 0.81 -0.7 4,008,025 - pldB b3825 0.81 +1.2 4,008,041 + pldB b3825 0.83 +0.0 4,008,054 - pldB b3825 0.84 +2.0 4,008,060 - pldB b3825 0.85 +0.8 4,008,066 + pldB b3825 0.85 +0.3 4,008,086 + pldB b3825 0.87 -0.1 4,008,086 + pldB b3825 0.87 -0.1 4,008,225 + +0.4 4,008,230 + -0.1 4,008,230 + +3.2 4,008,302 + -0.6 4,008,302 + -0.8 4,008,310 - yigL b3826 0.11 -0.5 4,008,350 + yigL b3826 0.16 -0.6 4,008,350 + yigL b3826 0.16 +0.5 4,008,393 - yigL b3826 0.21 -0.9 4,008,416 + yigL b3826 0.24 +2.0 4,008,430 + yigL b3826 0.26 -0.5 4,008,489 - yigL b3826 0.33 -1.6 4,008,495 + yigL b3826 0.34 +0.2 4,008,496 - yigL b3826 0.34 -1.1 4,008,505 + yigL b3826 0.35 +0.2 4,008,526 - yigL b3826 0.38 -0.3 4,008,526 - yigL b3826 0.38 -1.1 4,008,529 + yigL b3826 0.38 -2.5 4,008,572 + yigL b3826 0.44 -0.6 4,008,572 + yigL b3826 0.44 -1.3 4,008,621 + yigL b3826 0.50 -1.5 4,008,629 - yigL b3826 0.51 +0.1 4,008,629 - yigL b3826 0.51 +1.9 4,008,629 - yigL b3826 0.51 +1.7 4,008,632 + yigL b3826 0.51 +1.1 4,008,677 + yigL b3826 0.57 -0.1 4,008,677 + yigL b3826 0.57 -0.1 4,008,680 + yigL b3826 0.57 +0.5 4,008,688 - yigL b3826 0.58 +0.2 4,008,708 + yigL b3826 0.61 +1.4 4,008,754 + yigL b3826 0.66 +0.3 4,008,754 + yigL b3826 0.66 -2.6 4,008,754 + yigL b3826 0.66 -0.4 4,008,754 + yigL b3826 0.66 +0.4 4,008,762 - yigL b3826 0.67 -0.1 4,008,762 - yigL b3826 0.67 -0.3 4,008,903 - yigL b3826 0.85 -0.2 4,008,910 + yigL b3826 0.86 +0.6 4,008,918 - yigL b3826 0.87 -1.5 4,008,920 + yigL b3826 0.87 +1.9 4,008,920 + yigL b3826 0.87 -0.8 4,008,920 + yigL b3826 0.87 -2.6 4,008,968 + -0.3 4,009,067 - +0.6 4,009,082 - +0.2 4,009,134 - -2.0 4,009,176 - +1.6 4,009,176 - -0.9 4,009,205 - yigM b3827 0.12 -1.2 4,009,275 + yigM b3827 0.20 +0.2 4,009,275 + yigM b3827 0.20 -0.9 4,009,275 + yigM b3827 0.20 -0.1 4,009,275 + yigM b3827 0.20 -0.5 4,009,275 + yigM b3827 0.20 -1.9 4,009,275 + yigM b3827 0.20 -1.6 4,009,283 - yigM b3827 0.20 -1.8 4,009,283 - yigM b3827 0.20 +2.1 4,009,311 - yigM b3827 0.24 +1.0 4,009,334 + yigM b3827 0.26 +1.1 4,009,334 + yigM b3827 0.26 +1.0 4,009,338 - yigM b3827 0.27 -0.1 4,009,348 - yigM b3827 0.28 -0.1 4,009,348 - yigM b3827 0.28 -1.6 4,009,406 - yigM b3827 0.34 +1.4 4,009,428 + yigM b3827 0.37 -1.2 4,009,434 + yigM b3827 0.37 +1.2 4,009,434 + yigM b3827 0.37 -0.3 4,009,451 - yigM b3827 0.39 +1.5 4,009,495 - yigM b3827 0.44 -0.6 4,009,495 - yigM b3827 0.44 -0.5 4,009,506 + yigM b3827 0.45 -1.5 4,009,524 - yigM b3827 0.47 -0.8 4,009,600 + yigM b3827 0.56 -1.5 4,009,772 + yigM b3827 0.75 +0.7 4,009,839 - yigM b3827 0.82 -0.5 4,009,839 - yigM b3827 0.82 +0.5 4,009,845 - yigM b3827 0.83 -0.3 4,009,845 - yigM b3827 0.83 +1.3 4,009,874 + yigM b3827 0.86 +0.3 4,009,904 + -0.8 4,009,917 - +0.2 4,009,921 - +2.3 4,009,921 - +4.4 4,009,947 - +1.2
Or see this region's nucleotide sequence