Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjiC and yjiD are separated by 672 nucleotides yjiD and yjiE overlap by 8 nucleotides yjiE and iadA are separated by 64 nucleotides
b4325: yjiC - orf, hypothetical protein (VIMSS), at 4,553,513 to 4,554,343
yjiC
b4326: yjiD - orf, hypothetical protein (VIMSS), at 4,555,016 to 4,555,408
yjiD
b4327: yjiE - predicted DNA-binding transcriptional regulator (NCBI), at 4,555,401 to 4,556,312
yjiE
b4328: iadA - isoaspartyl dipeptidase (NCBI), at 4,556,377 to 4,557,549
iadA
Position (kb)
4555
4556 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4554.070 kb on - strand, within yjiC at 4554.733 kb on - strand at 4554.736 kb on + strand at 4554.736 kb on + strand at 4554.736 kb on + strand at 4554.847 kb on + strand at 4554.866 kb on - strand at 4554.884 kb on + strand at 4554.886 kb on - strand at 4554.892 kb on - strand at 4554.892 kb on - strand at 4554.894 kb on + strand at 4554.894 kb on + strand at 4554.894 kb on + strand at 4554.894 kb on + strand at 4554.895 kb on + strand at 4554.895 kb on + strand at 4554.902 kb on - strand at 4554.905 kb on + strand at 4554.908 kb on - strand at 4554.908 kb on - strand at 4554.917 kb on - strand at 4554.917 kb on - strand at 4554.940 kb on - strand at 4554.940 kb on - strand at 4554.950 kb on + strand at 4554.950 kb on + strand at 4554.950 kb on + strand at 4554.950 kb on + strand at 4554.950 kb on + strand at 4554.951 kb on + strand at 4554.951 kb on + strand at 4554.953 kb on + strand at 4554.953 kb on + strand at 4554.956 kb on - strand at 4554.958 kb on - strand at 4554.968 kb on + strand at 4554.970 kb on + strand at 4554.970 kb on + strand at 4554.978 kb on - strand at 4554.984 kb on + strand at 4554.999 kb on + strand at 4554.999 kb on + strand at 4555.090 kb on + strand, within yjiD at 4555.110 kb on - strand, within yjiD at 4555.149 kb on + strand, within yjiD at 4555.157 kb on - strand, within yjiD at 4555.161 kb on - strand, within yjiD at 4555.161 kb on - strand, within yjiD at 4555.164 kb on + strand, within yjiD at 4555.166 kb on - strand, within yjiD at 4555.172 kb on + strand, within yjiD at 4555.180 kb on - strand, within yjiD at 4555.181 kb on - strand, within yjiD at 4555.196 kb on - strand, within yjiD at 4555.204 kb on - strand, within yjiD at 4555.207 kb on + strand, within yjiD at 4555.207 kb on + strand, within yjiD at 4555.234 kb on + strand, within yjiD at 4555.246 kb on + strand, within yjiD at 4555.246 kb on + strand, within yjiD at 4555.273 kb on + strand, within yjiD at 4555.280 kb on + strand, within yjiD at 4555.282 kb on + strand, within yjiD at 4555.290 kb on + strand, within yjiD at 4555.296 kb on + strand, within yjiD at 4555.296 kb on + strand, within yjiD at 4555.296 kb on - strand, within yjiD at 4555.296 kb on - strand, within yjiD at 4555.308 kb on + strand, within yjiD at 4555.308 kb on + strand, within yjiD at 4555.308 kb on + strand, within yjiD at 4555.308 kb on + strand, within yjiD at 4555.314 kb on + strand at 4555.316 kb on - strand, within yjiD at 4555.316 kb on - strand, within yjiD at 4555.327 kb on - strand, within yjiD at 4555.327 kb on - strand, within yjiD at 4555.332 kb on - strand, within yjiD at 4555.332 kb on - strand, within yjiD at 4555.398 kb on - strand at 4555.407 kb on - strand at 4555.415 kb on + strand at 4555.415 kb on + strand at 4555.498 kb on + strand at 4555.498 kb on + strand, within yjiE at 4555.667 kb on + strand, within yjiE at 4555.731 kb on + strand, within yjiE at 4555.739 kb on - strand at 4555.764 kb on - strand, within yjiE at 4555.764 kb on - strand, within yjiE at 4555.791 kb on - strand, within yjiE at 4555.976 kb on + strand, within yjiE at 4556.042 kb on - strand, within yjiE at 4556.106 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.168 kb on + strand, within yjiE at 4556.334 kb on - strand at 4556.382 kb on - strand at 4556.382 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,554,070 - yjiC b4325 0.67 +1.0 4,554,733 - +0.3 4,554,736 + -1.0 4,554,736 + +0.3 4,554,736 + +1.8 4,554,847 + +1.9 4,554,866 - +1.4 4,554,884 + +0.3 4,554,886 - -0.2 4,554,892 - +1.4 4,554,892 - -0.2 4,554,894 + -1.6 4,554,894 + -2.1 4,554,894 + -0.9 4,554,894 + +1.2 4,554,895 + +1.4 4,554,895 + +0.4 4,554,902 - +1.5 4,554,905 + -1.2 4,554,908 - +1.2 4,554,908 - +0.8 4,554,917 - +0.8 4,554,917 - -0.6 4,554,940 - -1.2 4,554,940 - -0.7 4,554,950 + -0.6 4,554,950 + -0.9 4,554,950 + +0.4 4,554,950 + -0.9 4,554,950 + +0.8 4,554,951 + +2.1 4,554,951 + -2.1 4,554,953 + +2.5 4,554,953 + +3.5 4,554,956 - +0.8 4,554,958 - +1.4 4,554,968 + +1.6 4,554,970 + -0.8 4,554,970 + +0.6 4,554,978 - +2.6 4,554,984 + -2.7 4,554,999 + -0.9 4,554,999 + -1.4 4,555,090 + yjiD b4326 0.19 +1.6 4,555,110 - yjiD b4326 0.24 -0.7 4,555,149 + yjiD b4326 0.34 -1.6 4,555,157 - yjiD b4326 0.36 +0.1 4,555,161 - yjiD b4326 0.37 +1.8 4,555,161 - yjiD b4326 0.37 -0.5 4,555,164 + yjiD b4326 0.38 -1.7 4,555,166 - yjiD b4326 0.38 +3.0 4,555,172 + yjiD b4326 0.40 +1.6 4,555,180 - yjiD b4326 0.42 -0.4 4,555,181 - yjiD b4326 0.42 +3.4 4,555,196 - yjiD b4326 0.46 +0.7 4,555,204 - yjiD b4326 0.48 +0.9 4,555,207 + yjiD b4326 0.49 -0.2 4,555,207 + yjiD b4326 0.49 -0.9 4,555,234 + yjiD b4326 0.55 +0.2 4,555,246 + yjiD b4326 0.59 +0.2 4,555,246 + yjiD b4326 0.59 -0.4 4,555,273 + yjiD b4326 0.65 -0.6 4,555,280 + yjiD b4326 0.67 +0.7 4,555,282 + yjiD b4326 0.68 +0.8 4,555,290 + yjiD b4326 0.70 +1.4 4,555,296 + yjiD b4326 0.71 +0.5 4,555,296 + yjiD b4326 0.71 -0.2 4,555,296 - yjiD b4326 0.71 -0.1 4,555,296 - yjiD b4326 0.71 +1.6 4,555,308 + yjiD b4326 0.74 +3.3 4,555,308 + yjiD b4326 0.74 +2.1 4,555,308 + yjiD b4326 0.74 -1.6 4,555,308 + yjiD b4326 0.74 +1.5 4,555,314 + +1.4 4,555,316 - yjiD b4326 0.76 -1.4 4,555,316 - yjiD b4326 0.76 +0.4 4,555,327 - yjiD b4326 0.79 +0.9 4,555,327 - yjiD b4326 0.79 -1.4 4,555,332 - yjiD b4326 0.80 -0.2 4,555,332 - yjiD b4326 0.80 -0.4 4,555,398 - -1.3 4,555,407 - +0.8 4,555,415 + +0.9 4,555,415 + +2.1 4,555,498 + +0.4 4,555,498 + yjiE b4327 0.11 -1.0 4,555,667 + yjiE b4327 0.29 +1.3 4,555,731 + yjiE b4327 0.36 -0.9 4,555,739 - -0.6 4,555,764 - yjiE b4327 0.40 +0.9 4,555,764 - yjiE b4327 0.40 +0.9 4,555,791 - yjiE b4327 0.43 -0.4 4,555,976 + yjiE b4327 0.63 +1.0 4,556,042 - yjiE b4327 0.70 +0.1 4,556,106 + yjiE b4327 0.77 -0.2 4,556,168 + yjiE b4327 0.84 +0.1 4,556,168 + yjiE b4327 0.84 +0.6 4,556,334 - -0.1 4,556,382 - +1.1 4,556,382 - +1.5
Or see this region's nucleotide sequence