Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgaT and ulaB are separated by 15 nucleotides ulaB and ulaC are separated by 9 nucleotides ulaC and ulaD are separated by 13 nucleotides
b4193: sgaT - orf, hypothetical protein (VIMSS), at 4,418,003 to 4,419,400
sgaT
b4194: ulaB - L-ascorbate-specific enzyme IIB component of PTS (NCBI), at 4,419,416 to 4,419,721
ulaB
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
Position (kb)
4419
4420 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4418.474 kb on + strand, within sgaT at 4418.475 kb on + strand, within sgaT at 4418.579 kb on + strand, within sgaT at 4418.649 kb on + strand, within sgaT at 4418.692 kb on + strand, within sgaT at 4418.692 kb on + strand, within sgaT at 4418.736 kb on + strand, within sgaT at 4418.776 kb on - strand, within sgaT at 4418.787 kb on - strand, within sgaT at 4418.787 kb on - strand, within sgaT at 4418.798 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.801 kb on + strand, within sgaT at 4418.809 kb on - strand, within sgaT at 4418.809 kb on - strand, within sgaT at 4418.882 kb on - strand, within sgaT at 4418.914 kb on + strand, within sgaT at 4418.946 kb on + strand, within sgaT at 4419.069 kb on + strand, within sgaT at 4419.069 kb on + strand, within sgaT at 4419.148 kb on - strand, within sgaT at 4419.170 kb on + strand, within sgaT at 4419.245 kb on + strand, within sgaT at 4419.291 kb on + strand at 4419.357 kb on + strand at 4419.357 kb on + strand at 4419.431 kb on - strand at 4419.457 kb on - strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.630 kb on + strand, within ulaB at 4419.869 kb on + strand, within ulaC at 4419.869 kb on + strand, within ulaC at 4419.924 kb on - strand, within ulaC at 4419.929 kb on - strand, within ulaC at 4419.947 kb on - strand, within ulaC at 4420.137 kb on + strand, within ulaC at 4420.164 kb on + strand at 4420.164 kb on + strand at 4420.206 kb on + strand at 4420.355 kb on - strand, within ulaD at 4420.363 kb on + strand, within ulaD at 4420.386 kb on - strand, within ulaD at 4420.479 kb on + strand, within ulaD at 4420.533 kb on + strand, within ulaD at 4420.641 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,418,474 + sgaT b4193 0.34 -1.8 4,418,475 + sgaT b4193 0.34 +0.1 4,418,579 + sgaT b4193 0.41 +1.1 4,418,649 + sgaT b4193 0.46 -2.1 4,418,692 + sgaT b4193 0.49 -0.7 4,418,692 + sgaT b4193 0.49 +2.0 4,418,736 + sgaT b4193 0.52 +0.4 4,418,776 - sgaT b4193 0.55 -1.5 4,418,787 - sgaT b4193 0.56 -0.5 4,418,787 - sgaT b4193 0.56 +1.4 4,418,798 + sgaT b4193 0.57 +1.1 4,418,801 + sgaT b4193 0.57 -1.4 4,418,801 + sgaT b4193 0.57 +1.1 4,418,801 + sgaT b4193 0.57 +1.1 4,418,801 + sgaT b4193 0.57 +0.6 4,418,809 - sgaT b4193 0.58 -0.4 4,418,809 - sgaT b4193 0.58 -0.1 4,418,882 - sgaT b4193 0.63 -0.9 4,418,914 + sgaT b4193 0.65 +1.1 4,418,946 + sgaT b4193 0.67 +0.4 4,419,069 + sgaT b4193 0.76 +2.1 4,419,069 + sgaT b4193 0.76 +0.1 4,419,148 - sgaT b4193 0.82 -0.6 4,419,170 + sgaT b4193 0.83 +2.5 4,419,245 + sgaT b4193 0.89 -1.2 4,419,291 + +1.2 4,419,357 + +0.7 4,419,357 + -0.2 4,419,431 - +0.5 4,419,457 - ulaB b4194 0.13 +1.4 4,419,584 + ulaB b4194 0.55 +0.6 4,419,584 + ulaB b4194 0.55 +4.1 4,419,584 + ulaB b4194 0.55 -0.4 4,419,630 + ulaB b4194 0.70 +1.4 4,419,869 + ulaC b4195 0.30 -1.8 4,419,869 + ulaC b4195 0.30 +1.8 4,419,924 - ulaC b4195 0.42 +0.6 4,419,929 - ulaC b4195 0.43 +1.1 4,419,947 - ulaC b4195 0.46 -0.6 4,420,137 + ulaC b4195 0.87 -0.9 4,420,164 + +1.4 4,420,164 + -0.4 4,420,206 + -0.2 4,420,355 - ulaD b4196 0.22 -0.6 4,420,363 + ulaD b4196 0.24 +0.4 4,420,386 - ulaD b4196 0.27 +0.1 4,420,479 + ulaD b4196 0.41 -0.1 4,420,533 + ulaD b4196 0.50 +2.2 4,420,641 + ulaD b4196 0.66 +1.4 4,420,719 + ulaD b4196 0.78 +2.1 4,420,719 + ulaD b4196 0.78 +2.1
Or see this region's nucleotide sequence