Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt amiB and mutL are separated by 9 nucleotides mutL and miaA overlap by 8 nucleotides
b4169: amiB - N-acetylmuramoyl-l-alanine amidase II (NCBI), at 4,394,088 to 4,395,425
amiB
b4170: mutL - DNA mismatch repair protein (NCBI), at 4,395,435 to 4,397,282
mutL
b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225
miaA
Position (kb)
4395
4396
4397
4398 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4394.454 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.553 kb on + strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.603 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.606 kb on - strand, within amiB at 4394.642 kb on + strand, within amiB at 4394.650 kb on - strand, within amiB at 4394.755 kb on + strand, within amiB at 4394.798 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.832 kb on - strand, within amiB at 4394.850 kb on + strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.856 kb on - strand, within amiB at 4394.912 kb on + strand, within amiB at 4394.917 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.939 kb on - strand, within amiB at 4394.942 kb on + strand, within amiB at 4394.943 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.944 kb on + strand, within amiB at 4394.951 kb on - strand, within amiB at 4394.951 kb on - strand, within amiB at 4395.041 kb on + strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.080 kb on - strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.112 kb on + strand, within amiB at 4395.116 kb on - strand, within amiB at 4395.214 kb on + strand, within amiB at 4395.217 kb on + strand, within amiB at 4395.246 kb on + strand, within amiB at 4395.286 kb on + strand, within amiB at 4395.307 kb on - strand at 4395.307 kb on - strand at 4395.348 kb on + strand at 4395.348 kb on + strand at 4395.414 kb on - strand at 4395.454 kb on + strand at 4395.596 kb on - strand at 4395.655 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.679 kb on + strand, within mutL at 4395.775 kb on + strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.808 kb on - strand, within mutL at 4395.853 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.854 kb on + strand, within mutL at 4395.861 kb on - strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.865 kb on + strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.872 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.873 kb on - strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.920 kb on + strand, within mutL at 4395.986 kb on - strand, within mutL at 4395.986 kb on - strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.002 kb on + strand, within mutL at 4396.079 kb on + strand, within mutL at 4396.148 kb on - strand, within mutL at 4396.248 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.271 kb on + strand, within mutL at 4396.326 kb on - strand, within mutL at 4396.329 kb on - strand, within mutL at 4396.407 kb on + strand, within mutL at 4396.415 kb on + strand, within mutL at 4396.415 kb on - strand, within mutL at 4396.432 kb on - strand, within mutL at 4396.435 kb on - strand, within mutL at 4396.435 kb on - strand, within mutL at 4396.466 kb on - strand, within mutL at 4396.546 kb on + strand, within mutL at 4396.551 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.556 kb on + strand, within mutL at 4396.778 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.991 kb on + strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4396.999 kb on - strand, within mutL at 4397.012 kb on + strand, within mutL at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.162 kb on + strand at 4397.170 kb on - strand at 4397.195 kb on - strand at 4397.195 kb on - strand at 4397.244 kb on + strand at 4397.244 kb on + strand at 4397.420 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.449 kb on + strand, within miaA at 4397.455 kb on + strand, within miaA at 4397.458 kb on - strand, within miaA at 4397.505 kb on + strand, within miaA at 4397.549 kb on - strand, within miaA at 4397.584 kb on + strand, within miaA at 4397.589 kb on - strand, within miaA at 4397.704 kb on - strand, within miaA at 4397.763 kb on - strand, within miaA at 4397.845 kb on - strand, within miaA at 4397.853 kb on + strand, within miaA at 4397.856 kb on + strand, within miaA at 4397.866 kb on + strand, within miaA at 4397.922 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4397.935 kb on + strand, within miaA at 4398.137 kb on + strand at 4398.137 kb on + strand at 4398.156 kb on + strand at 4398.233 kb on + strand at 4398.233 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,394,454 + amiB b4169 0.27 -1.6 4,394,553 + amiB b4169 0.35 -0.7 4,394,553 + amiB b4169 0.35 +1.3 4,394,603 - amiB b4169 0.38 +0.5 4,394,603 - amiB b4169 0.38 +3.3 4,394,606 - amiB b4169 0.39 -0.9 4,394,606 - amiB b4169 0.39 +1.8 4,394,642 + amiB b4169 0.41 -0.2 4,394,650 - amiB b4169 0.42 -1.6 4,394,755 + amiB b4169 0.50 -1.6 4,394,798 - amiB b4169 0.53 +0.2 4,394,832 - amiB b4169 0.56 +2.0 4,394,832 - amiB b4169 0.56 +1.2 4,394,850 + amiB b4169 0.57 -2.2 4,394,856 - amiB b4169 0.57 -2.1 4,394,856 - amiB b4169 0.57 -1.9 4,394,912 + amiB b4169 0.62 -0.4 4,394,917 - amiB b4169 0.62 -0.9 4,394,939 - amiB b4169 0.64 +0.1 4,394,939 - amiB b4169 0.64 -0.2 4,394,942 + amiB b4169 0.64 +0.1 4,394,943 + amiB b4169 0.64 -0.6 4,394,944 + amiB b4169 0.64 +0.8 4,394,944 + amiB b4169 0.64 +0.9 4,394,951 - amiB b4169 0.64 +0.7 4,394,951 - amiB b4169 0.64 -0.4 4,395,041 + amiB b4169 0.71 -3.3 4,395,080 - amiB b4169 0.74 -0.6 4,395,080 - amiB b4169 0.74 -0.0 4,395,112 + amiB b4169 0.77 -0.0 4,395,112 + amiB b4169 0.77 -0.8 4,395,116 - amiB b4169 0.77 -1.3 4,395,214 + amiB b4169 0.84 -1.2 4,395,217 + amiB b4169 0.84 -0.3 4,395,246 + amiB b4169 0.87 -1.0 4,395,286 + amiB b4169 0.90 -0.4 4,395,307 - +3.1 4,395,307 - +3.1 4,395,348 + -1.2 4,395,348 + +2.0 4,395,414 - +1.3 4,395,454 + +1.4 4,395,596 - +0.0 4,395,655 + mutL b4170 0.12 +1.4 4,395,679 + mutL b4170 0.13 -1.1 4,395,679 + mutL b4170 0.13 -0.6 4,395,775 + mutL b4170 0.18 +0.4 4,395,808 - mutL b4170 0.20 +1.7 4,395,808 - mutL b4170 0.20 -1.0 4,395,808 - mutL b4170 0.20 -0.9 4,395,853 + mutL b4170 0.23 -0.2 4,395,854 + mutL b4170 0.23 +3.9 4,395,854 + mutL b4170 0.23 +1.4 4,395,861 - mutL b4170 0.23 +2.4 4,395,865 + mutL b4170 0.23 -1.4 4,395,865 + mutL b4170 0.23 +0.7 4,395,872 - mutL b4170 0.24 -0.4 4,395,872 - mutL b4170 0.24 -1.2 4,395,873 - mutL b4170 0.24 +2.0 4,395,873 - mutL b4170 0.24 +1.2 4,395,920 + mutL b4170 0.26 -0.9 4,395,920 + mutL b4170 0.26 +0.1 4,395,986 - mutL b4170 0.30 +1.4 4,395,986 - mutL b4170 0.30 +0.7 4,396,002 + mutL b4170 0.31 +0.7 4,396,002 + mutL b4170 0.31 +1.0 4,396,079 + mutL b4170 0.35 +1.4 4,396,148 - mutL b4170 0.39 +2.4 4,396,248 + mutL b4170 0.44 +2.2 4,396,271 + mutL b4170 0.45 +0.4 4,396,271 + mutL b4170 0.45 -0.7 4,396,326 - mutL b4170 0.48 +0.6 4,396,329 - mutL b4170 0.48 +2.4 4,396,407 + mutL b4170 0.53 +0.6 4,396,415 + mutL b4170 0.53 -0.8 4,396,415 - mutL b4170 0.53 +0.7 4,396,432 - mutL b4170 0.54 +1.8 4,396,435 - mutL b4170 0.54 +0.8 4,396,435 - mutL b4170 0.54 -0.2 4,396,466 - mutL b4170 0.56 +2.9 4,396,546 + mutL b4170 0.60 -0.1 4,396,551 + mutL b4170 0.60 +0.6 4,396,556 + mutL b4170 0.61 -0.5 4,396,556 + mutL b4170 0.61 +0.8 4,396,556 + mutL b4170 0.61 +1.4 4,396,778 + mutL b4170 0.73 +0.4 4,396,991 + mutL b4170 0.84 +0.9 4,396,991 + mutL b4170 0.84 +1.7 4,396,991 + mutL b4170 0.84 +1.8 4,396,999 - mutL b4170 0.85 +2.0 4,396,999 - mutL b4170 0.85 +0.5 4,396,999 - mutL b4170 0.85 +0.4 4,396,999 - mutL b4170 0.85 -0.8 4,397,012 + mutL b4170 0.85 -0.4 4,397,162 + +2.6 4,397,162 + +0.9 4,397,162 + +0.4 4,397,162 + +1.5 4,397,170 - +1.2 4,397,195 - +0.2 4,397,195 - -0.6 4,397,244 + +0.6 4,397,244 + +2.4 4,397,420 + miaA b4171 0.15 -2.6 4,397,449 + miaA b4171 0.18 -0.2 4,397,449 + miaA b4171 0.18 -0.9 4,397,455 + miaA b4171 0.19 +0.4 4,397,458 - miaA b4171 0.19 -0.5 4,397,505 + miaA b4171 0.24 -1.4 4,397,549 - miaA b4171 0.29 -0.2 4,397,584 + miaA b4171 0.32 -0.6 4,397,589 - miaA b4171 0.33 -2.5 4,397,704 - miaA b4171 0.45 -1.8 4,397,763 - miaA b4171 0.51 -0.6 4,397,845 - miaA b4171 0.60 -0.9 4,397,853 + miaA b4171 0.61 +4.0 4,397,856 + miaA b4171 0.61 -1.2 4,397,866 + miaA b4171 0.62 -1.1 4,397,922 + miaA b4171 0.68 -1.1 4,397,935 + miaA b4171 0.69 -0.3 4,397,935 + miaA b4171 0.69 -1.3 4,398,137 + -1.9 4,398,137 + -2.3 4,398,156 + +0.4 4,398,233 + +2.5 4,398,233 + -1.6
Or see this region's nucleotide sequence