Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnP and phnO are separated by 1 nucleotides phnO and phnN overlap by 14 nucleotides phnN and phnM overlap by 1 nucleotides
b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125
phnP
b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561
phnO
b4094: phnN - ribose 1,5-bisphosphokinase (NCBI), at 4,313,548 to 4,314,105
phnN
b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241
phnM
Position (kb) 4313
4314
4315 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 4312.567 kb on + strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.700 kb on + strand, within phnP at 4312.714 kb on - strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.721 kb on - strand, within phnP at 4312.728 kb on - strand, within phnP at 4312.788 kb on - strand, within phnP at 4312.803 kb on + strand, within phnP at 4312.808 kb on + strand, within phnP at 4312.948 kb on - strand, within phnP at 4312.965 kb on - strand, within phnP at 4313.024 kb on - strand, within phnP at 4313.041 kb on + strand, within phnP at 4313.060 kb on + strand at 4313.094 kb on - strand at 4313.115 kb on - strand at 4313.115 kb on - strand at 4313.127 kb on + strand at 4313.185 kb on - strand, within phnO at 4313.195 kb on - strand, within phnO at 4313.274 kb on + strand, within phnO at 4313.433 kb on + strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.529 kb on - strand at 4313.529 kb on - strand at 4313.565 kb on - strand at 4313.634 kb on + strand, within phnN at 4313.662 kb on - strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.956 kb on - strand, within phnN at 4314.001 kb on - strand, within phnN at 4314.043 kb on + strand, within phnN at 4314.122 kb on - strand at 4314.213 kb on + strand at 4314.213 kb on + strand at 4314.357 kb on - strand, within phnM at 4314.363 kb on + strand, within phnM at 4314.396 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.742 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4315.006 kb on - strand, within phnM
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,312,567 + phnP b4092 0.26 +5.8 4,312,658 - phnP b4092 0.38 +0.4 4,312,658 - phnP b4092 0.38 -1.4 4,312,663 - phnP b4092 0.39 +1.4 4,312,663 - phnP b4092 0.39 -1.7 4,312,668 - phnP b4092 0.40 +0.8 4,312,668 - phnP b4092 0.40 +0.9 4,312,700 + phnP b4092 0.44 +0.9 4,312,714 - phnP b4092 0.46 -0.0 4,312,720 + phnP b4092 0.47 +0.4 4,312,720 + phnP b4092 0.47 +0.4 4,312,721 - phnP b4092 0.47 +0.8 4,312,728 - phnP b4092 0.48 -1.8 4,312,788 - phnP b4092 0.55 +1.0 4,312,803 + phnP b4092 0.57 +0.4 4,312,808 + phnP b4092 0.58 -1.2 4,312,948 - phnP b4092 0.77 -0.3 4,312,965 - phnP b4092 0.79 -1.1 4,313,024 - phnP b4092 0.87 -0.3 4,313,041 + phnP b4092 0.89 -2.0 4,313,060 + +0.7 4,313,094 - -0.9 4,313,115 - -1.6 4,313,115 - -0.9 4,313,127 + -0.9 4,313,185 - phnO b4093 0.13 -0.4 4,313,195 - phnO b4093 0.16 +0.7 4,313,274 + phnO b4093 0.34 +0.9 4,313,433 + phnO b4093 0.70 +1.8 4,313,502 - phnO b4093 0.86 +0.4 4,313,502 - phnO b4093 0.86 +0.1 4,313,529 - +2.2 4,313,529 - +0.8 4,313,565 - -0.2 4,313,634 + phnN b4094 0.15 -2.0 4,313,662 - phnN b4094 0.20 -0.8 4,313,821 + phnN b4094 0.49 -0.2 4,313,821 + phnN b4094 0.49 -0.4 4,313,881 - phnN b4094 0.60 +0.4 4,313,881 - phnN b4094 0.60 +2.4 4,313,956 - phnN b4094 0.73 -2.7 4,314,001 - phnN b4094 0.81 +0.7 4,314,043 + phnN b4094 0.89 -1.9 4,314,122 - +1.7 4,314,213 + +2.0 4,314,213 + +0.7 4,314,357 - phnM b4095 0.22 -3.5 4,314,363 + phnM b4095 0.23 -1.2 4,314,396 + phnM b4095 0.26 +0.5 4,314,445 + phnM b4095 0.30 -2.3 4,314,445 + phnM b4095 0.30 +1.8 4,314,742 + phnM b4095 0.56 +0.2 4,314,819 + phnM b4095 0.63 -0.6 4,314,819 + phnM b4095 0.63 +0.4 4,314,822 - phnM b4095 0.63 +0.0 4,314,822 - phnM b4095 0.63 +0.9 4,314,857 - phnM b4095 0.66 -0.4 4,314,857 - phnM b4095 0.66 -0.3 4,315,006 - phnM b4095 0.79 +0.4
Or see this region's nucleotide sequence