Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpoC and htrC are separated by 212 nucleotides htrC and sroH are separated by 1 nucleotides sroH and thiH are separated by 247 nucleotides
b3988: rpoC - DNA-directed RNA polymerase subunit beta' (NCBI), at 4,183,373 to 4,187,596
rpoC
b3989: htrC - heat shock protein (NCBI), at 4,187,809 to 4,188,348
htrC
b4691: sroH - Novel sRNA, function unknown (RefSeq), at 4,188,350 to 4,188,510
sroH
b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891
thiH
Position (kb)
4187
4188
4189 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4187.467 kb on + strand at 4187.514 kb on + strand at 4187.514 kb on + strand at 4187.516 kb on + strand at 4187.516 kb on + strand at 4187.526 kb on + strand at 4187.526 kb on + strand at 4187.708 kb on - strand at 4187.708 kb on - strand at 4187.774 kb on + strand at 4187.774 kb on + strand at 4187.793 kb on + strand at 4187.896 kb on + strand, within htrC at 4187.904 kb on - strand, within htrC at 4187.904 kb on - strand, within htrC at 4187.916 kb on - strand, within htrC at 4187.949 kb on + strand, within htrC at 4187.957 kb on - strand, within htrC at 4187.957 kb on - strand, within htrC at 4187.994 kb on - strand, within htrC at 4187.994 kb on - strand, within htrC at 4188.013 kb on + strand, within htrC at 4188.013 kb on + strand, within htrC at 4188.034 kb on - strand, within htrC at 4188.034 kb on - strand, within htrC at 4188.049 kb on + strand, within htrC at 4188.066 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.087 kb on - strand, within htrC at 4188.130 kb on + strand, within htrC at 4188.150 kb on + strand, within htrC at 4188.160 kb on + strand, within htrC at 4188.168 kb on - strand, within htrC at 4188.168 kb on - strand, within htrC at 4188.196 kb on - strand, within htrC at 4188.239 kb on + strand, within htrC at 4188.309 kb on - strand at 4188.309 kb on - strand at 4188.365 kb on - strand at 4188.365 kb on - strand at 4188.565 kb on + strand at 4188.579 kb on - strand at 4188.751 kb on - strand at 4188.765 kb on - strand at 4188.812 kb on + strand at 4188.870 kb on + strand at 4188.899 kb on - strand, within thiH at 4189.035 kb on - strand, within thiH at 4189.048 kb on + strand, within thiH at 4189.056 kb on - strand, within thiH at 4189.073 kb on - strand, within thiH at 4189.133 kb on - strand, within thiH at 4189.334 kb on - strand, within thiH
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 4,187,467 + +0.5 4,187,514 + +0.2 4,187,514 + -0.8 4,187,516 + -1.3 4,187,516 + -0.1 4,187,526 + +0.9 4,187,526 + +0.2 4,187,708 - -1.8 4,187,708 - -1.7 4,187,774 + +0.7 4,187,774 + -1.0 4,187,793 + -0.5 4,187,896 + htrC b3989 0.16 -1.5 4,187,904 - htrC b3989 0.18 -0.6 4,187,904 - htrC b3989 0.18 +1.4 4,187,916 - htrC b3989 0.20 +0.9 4,187,949 + htrC b3989 0.26 -0.4 4,187,957 - htrC b3989 0.27 -0.6 4,187,957 - htrC b3989 0.27 -1.7 4,187,994 - htrC b3989 0.34 -0.8 4,187,994 - htrC b3989 0.34 -1.8 4,188,013 + htrC b3989 0.38 -2.0 4,188,013 + htrC b3989 0.38 -1.1 4,188,034 - htrC b3989 0.42 -1.5 4,188,034 - htrC b3989 0.42 -2.2 4,188,049 + htrC b3989 0.44 -0.4 4,188,066 - htrC b3989 0.48 -0.8 4,188,087 - htrC b3989 0.51 +0.7 4,188,087 - htrC b3989 0.51 -0.8 4,188,087 - htrC b3989 0.51 +1.5 4,188,130 + htrC b3989 0.59 +1.6 4,188,150 + htrC b3989 0.63 +0.0 4,188,160 + htrC b3989 0.65 -1.1 4,188,168 - htrC b3989 0.66 -1.3 4,188,168 - htrC b3989 0.66 +2.1 4,188,196 - htrC b3989 0.72 -0.8 4,188,239 + htrC b3989 0.80 -1.0 4,188,309 - -0.8 4,188,309 - +0.9 4,188,365 - +0.6 4,188,365 - -1.3 4,188,565 + +2.0 4,188,579 - +0.5 4,188,751 - -1.1 4,188,765 - -0.3 4,188,812 + +1.6 4,188,870 + +0.7 4,188,899 - thiH b3990 0.12 -1.1 4,189,035 - thiH b3990 0.24 +0.7 4,189,048 + thiH b3990 0.26 -1.4 4,189,056 - thiH b3990 0.26 +1.1 4,189,073 - thiH b3990 0.28 -0.4 4,189,133 - thiH b3990 0.33 -0.3 4,189,334 - thiH b3990 0.51 +2.5
Or see this region's nucleotide sequence