Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gyrB and recF are separated by 28 nucleotides recF and dnaN overlap by 1 nucleotides
b3699: gyrB - DNA gyrase subunit B (NCBI), at 3,875,728 to 3,878,142
gyrB
b3700: recF - recombination protein F (NCBI), at 3,878,171 to 3,879,244
recF
b3701: dnaN - DNA polymerase III subunit beta (NCBI), at 3,879,244 to 3,880,344
dnaN
Position (kb)
3878
3879
3880 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3878.207 kb on - strand at 3878.227 kb on + strand at 3878.228 kb on + strand at 3878.233 kb on - strand at 3878.247 kb on - strand at 3878.247 kb on - strand at 3878.257 kb on - strand at 3878.322 kb on - strand, within recF at 3878.376 kb on - strand, within recF at 3878.407 kb on - strand, within recF at 3878.407 kb on - strand, within recF at 3878.489 kb on + strand, within recF at 3878.503 kb on - strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.505 kb on + strand, within recF at 3878.513 kb on - strand, within recF at 3878.559 kb on - strand at 3878.559 kb on - strand at 3878.565 kb on - strand, within recF at 3878.628 kb on + strand, within recF at 3878.655 kb on + strand, within recF at 3878.655 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.657 kb on + strand, within recF at 3878.660 kb on + strand, within recF at 3878.665 kb on - strand, within recF at 3878.665 kb on - strand, within recF at 3878.699 kb on + strand, within recF at 3878.707 kb on + strand, within recF at 3878.714 kb on - strand, within recF at 3878.736 kb on - strand, within recF at 3878.736 kb on - strand, within recF at 3878.742 kb on + strand, within recF at 3878.757 kb on + strand, within recF at 3878.757 kb on + strand, within recF at 3878.760 kb on - strand, within recF at 3878.789 kb on + strand, within recF at 3878.837 kb on + strand, within recF at 3878.837 kb on + strand, within recF at 3878.878 kb on - strand, within recF at 3878.883 kb on - strand, within recF at 3878.912 kb on + strand, within recF at 3878.912 kb on + strand, within recF at 3878.915 kb on - strand, within recF at 3878.915 kb on - strand, within recF at 3878.926 kb on - strand, within recF at 3878.926 kb on - strand, within recF at 3878.941 kb on + strand, within recF at 3878.949 kb on - strand, within recF at 3878.954 kb on - strand, within recF at 3878.982 kb on + strand, within recF at 3878.991 kb on - strand, within recF at 3878.992 kb on + strand, within recF at 3879.003 kb on + strand, within recF at 3879.052 kb on + strand, within recF at 3879.052 kb on + strand, within recF at 3879.098 kb on + strand, within recF at 3879.099 kb on - strand, within recF at 3879.117 kb on - strand, within recF at 3879.119 kb on - strand, within recF at 3879.120 kb on - strand, within recF at 3879.120 kb on - strand, within recF at 3879.181 kb on - strand at 3879.185 kb on + strand at 3879.220 kb on - strand at 3879.242 kb on - strand at 3879.242 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,878,207 - -2.3 3,878,227 + -2.3 3,878,228 + -0.1 3,878,233 - -2.5 3,878,247 - -0.8 3,878,247 - -2.9 3,878,257 - -2.2 3,878,322 - recF b3700 0.14 -2.1 3,878,376 - recF b3700 0.19 +0.2 3,878,407 - recF b3700 0.22 -2.1 3,878,407 - recF b3700 0.22 -1.7 3,878,489 + recF b3700 0.30 -2.7 3,878,503 - recF b3700 0.31 -2.9 3,878,505 + recF b3700 0.31 -1.7 3,878,505 + recF b3700 0.31 -3.2 3,878,505 + recF b3700 0.31 -1.1 3,878,505 + recF b3700 0.31 -2.4 3,878,505 + recF b3700 0.31 -3.5 3,878,505 + recF b3700 0.31 -2.6 3,878,513 - recF b3700 0.32 -1.8 3,878,559 - +1.5 3,878,559 - +0.5 3,878,565 - recF b3700 0.37 -0.3 3,878,628 + recF b3700 0.43 -3.4 3,878,655 + recF b3700 0.45 -3.7 3,878,655 + recF b3700 0.45 -2.6 3,878,657 + recF b3700 0.45 -1.9 3,878,657 + recF b3700 0.45 -1.5 3,878,657 + recF b3700 0.45 -1.7 3,878,657 + recF b3700 0.45 -0.5 3,878,660 + recF b3700 0.46 -2.2 3,878,665 - recF b3700 0.46 -4.3 3,878,665 - recF b3700 0.46 -3.2 3,878,699 + recF b3700 0.49 -1.6 3,878,707 + recF b3700 0.50 -0.5 3,878,714 - recF b3700 0.51 -2.4 3,878,736 - recF b3700 0.53 -1.5 3,878,736 - recF b3700 0.53 -1.8 3,878,742 + recF b3700 0.53 -3.3 3,878,757 + recF b3700 0.55 -2.5 3,878,757 + recF b3700 0.55 -1.3 3,878,760 - recF b3700 0.55 -2.8 3,878,789 + recF b3700 0.58 -2.2 3,878,837 + recF b3700 0.62 -0.8 3,878,837 + recF b3700 0.62 -0.8 3,878,878 - recF b3700 0.66 -3.5 3,878,883 - recF b3700 0.66 -1.1 3,878,912 + recF b3700 0.69 -1.3 3,878,912 + recF b3700 0.69 -1.9 3,878,915 - recF b3700 0.69 -2.3 3,878,915 - recF b3700 0.69 +0.5 3,878,926 - recF b3700 0.70 -0.5 3,878,926 - recF b3700 0.70 -1.3 3,878,941 + recF b3700 0.72 -1.8 3,878,949 - recF b3700 0.72 -0.8 3,878,954 - recF b3700 0.73 -2.7 3,878,982 + recF b3700 0.76 -1.3 3,878,991 - recF b3700 0.76 -1.1 3,878,992 + recF b3700 0.76 -1.7 3,879,003 + recF b3700 0.77 -2.4 3,879,052 + recF b3700 0.82 -1.9 3,879,052 + recF b3700 0.82 -1.9 3,879,098 + recF b3700 0.86 -0.8 3,879,099 - recF b3700 0.86 +0.5 3,879,117 - recF b3700 0.88 -2.1 3,879,119 - recF b3700 0.88 -2.4 3,879,120 - recF b3700 0.88 -1.5 3,879,120 - recF b3700 0.88 -2.3 3,879,181 - -1.6 3,879,185 + -1.3 3,879,220 - -2.2 3,879,242 - +0.5 3,879,242 - -1.1
Or see this region's nucleotide sequence