Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uhpC and uhpB are separated by 9 nucleotides uhpB and uhpA overlap by 1 nucleotides uhpA and ilvN are separated by 75 nucleotides ilvN and ilvB are separated by 3 nucleotides
b3667: uhpC - regulator of uhpT (VIMSS), at 3,845,328 to 3,846,647
uhpC
b3668: uhpB - sensor histidine protein kinase phosphorylates UhpA (VIMSS), at 3,846,657 to 3,848,159
uhpB
b3669: uhpA - DNA-binding response regulator in two-component regulatory system wtih UhpB (NCBI), at 3,848,159 to 3,848,749
uhpA
b3670: ilvN - acetolactate synthase small subunit (NCBI), at 3,848,825 to 3,849,115
ilvN
b3671: ilvB - acetolactate synthase large subunit (NCBI), at 3,849,119 to 3,850,807
ilvB
Position (kb)
3846
3847
3848
3849 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 3845.709 kb on - strand, within uhpC at 3845.709 kb on - strand, within uhpC at 3845.719 kb on - strand, within uhpC at 3845.776 kb on + strand, within uhpC at 3845.809 kb on + strand, within uhpC at 3845.809 kb on + strand, within uhpC at 3845.855 kb on + strand, within uhpC at 3845.857 kb on + strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.865 kb on - strand, within uhpC at 3845.950 kb on - strand, within uhpC at 3845.968 kb on + strand, within uhpC at 3845.991 kb on + strand, within uhpC at 3846.103 kb on + strand, within uhpC at 3846.133 kb on + strand, within uhpC at 3846.171 kb on + strand, within uhpC at 3846.171 kb on + strand, within uhpC at 3846.176 kb on - strand, within uhpC at 3846.176 kb on - strand, within uhpC at 3846.236 kb on - strand, within uhpC at 3846.271 kb on + strand, within uhpC at 3846.271 kb on + strand, within uhpC at 3846.284 kb on - strand, within uhpC at 3846.344 kb on + strand, within uhpC at 3846.344 kb on + strand, within uhpC at 3846.347 kb on - strand, within uhpC at 3846.410 kb on - strand, within uhpC at 3846.411 kb on + strand, within uhpC at 3846.418 kb on - strand, within uhpC at 3846.419 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.429 kb on - strand, within uhpC at 3846.554 kb on - strand at 3846.604 kb on + strand at 3846.655 kb on + strand at 3846.674 kb on - strand at 3846.674 kb on - strand at 3846.685 kb on - strand at 3846.689 kb on + strand at 3846.815 kb on - strand, within uhpB at 3846.815 kb on - strand, within uhpB at 3846.965 kb on + strand, within uhpB at 3846.965 kb on + strand, within uhpB at 3846.983 kb on - strand, within uhpB at 3846.983 kb on - strand, within uhpB at 3847.125 kb on + strand, within uhpB at 3847.125 kb on + strand, within uhpB at 3847.172 kb on - strand at 3847.174 kb on + strand, within uhpB at 3847.190 kb on + strand, within uhpB at 3847.203 kb on + strand, within uhpB at 3847.243 kb on - strand, within uhpB at 3847.253 kb on - strand, within uhpB at 3847.264 kb on - strand, within uhpB at 3847.327 kb on + strand, within uhpB at 3847.370 kb on + strand, within uhpB at 3847.422 kb on - strand, within uhpB at 3847.422 kb on - strand, within uhpB at 3847.464 kb on + strand, within uhpB at 3847.480 kb on + strand, within uhpB at 3847.488 kb on - strand, within uhpB at 3847.494 kb on - strand, within uhpB at 3847.516 kb on + strand, within uhpB at 3847.593 kb on + strand, within uhpB at 3847.593 kb on + strand, within uhpB at 3847.665 kb on - strand, within uhpB at 3847.665 kb on - strand, within uhpB at 3847.723 kb on - strand, within uhpB at 3847.815 kb on + strand, within uhpB at 3847.815 kb on + strand, within uhpB at 3847.825 kb on - strand, within uhpB at 3847.825 kb on - strand, within uhpB at 3847.828 kb on - strand, within uhpB at 3847.828 kb on - strand, within uhpB at 3847.880 kb on - strand, within uhpB at 3847.883 kb on - strand, within uhpB at 3847.883 kb on - strand, within uhpB at 3847.942 kb on + strand, within uhpB at 3848.011 kb on - strand at 3848.016 kb on - strand at 3848.029 kb on - strand at 3848.029 kb on - strand at 3848.043 kb on + strand at 3848.045 kb on + strand at 3848.047 kb on + strand at 3848.074 kb on - strand at 3848.074 kb on - strand at 3848.083 kb on - strand at 3848.094 kb on - strand at 3848.129 kb on + strand at 3848.129 kb on + strand at 3848.163 kb on - strand at 3848.165 kb on + strand at 3848.193 kb on + strand at 3848.193 kb on + strand at 3848.196 kb on - strand at 3848.237 kb on + strand, within uhpA at 3848.250 kb on - strand, within uhpA at 3848.261 kb on + strand, within uhpA at 3848.285 kb on - strand, within uhpA at 3848.370 kb on - strand, within uhpA at 3848.439 kb on + strand, within uhpA at 3848.492 kb on - strand, within uhpA at 3848.492 kb on - strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.494 kb on + strand, within uhpA at 3848.504 kb on + strand, within uhpA at 3848.504 kb on + strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.524 kb on - strand, within uhpA at 3848.526 kb on - strand, within uhpA at 3848.565 kb on - strand, within uhpA at 3848.565 kb on - strand, within uhpA at 3848.571 kb on + strand, within uhpA at 3848.571 kb on + strand, within uhpA at 3848.577 kb on + strand, within uhpA at 3848.585 kb on - strand, within uhpA at 3848.595 kb on + strand, within uhpA at 3848.603 kb on - strand, within uhpA at 3848.608 kb on - strand, within uhpA at 3848.610 kb on - strand, within uhpA at 3848.719 kb on + strand at 3848.734 kb on + strand at 3848.904 kb on - strand, within ilvN at 3848.921 kb on + strand, within ilvN at 3848.950 kb on + strand, within ilvN at 3848.953 kb on + strand, within ilvN at 3848.953 kb on + strand, within ilvN at 3848.958 kb on - strand, within ilvN at 3848.958 kb on - strand, within ilvN at 3848.966 kb on + strand, within ilvN at 3848.978 kb on + strand, within ilvN at 3848.978 kb on + strand, within ilvN at 3848.986 kb on - strand, within ilvN at 3849.000 kb on - strand, within ilvN at 3849.000 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.044 kb on - strand, within ilvN at 3849.050 kb on + strand, within ilvN at 3849.050 kb on + strand, within ilvN at 3849.055 kb on - strand, within ilvN at 3849.103 kb on - strand at 3849.103 kb on - strand at 3849.110 kb on - strand at 3849.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,845,709 - uhpC b3667 0.29 +0.9 3,845,709 - uhpC b3667 0.29 +1.4 3,845,719 - uhpC b3667 0.30 +5.8 3,845,776 + uhpC b3667 0.34 +2.2 3,845,809 + uhpC b3667 0.36 +1.1 3,845,809 + uhpC b3667 0.36 +1.8 3,845,855 + uhpC b3667 0.40 -0.7 3,845,857 + uhpC b3667 0.40 -0.7 3,845,865 - uhpC b3667 0.41 +1.4 3,845,865 - uhpC b3667 0.41 -0.5 3,845,865 - uhpC b3667 0.41 -0.2 3,845,950 - uhpC b3667 0.47 +1.7 3,845,968 + uhpC b3667 0.48 +1.4 3,845,991 + uhpC b3667 0.50 -1.0 3,846,103 + uhpC b3667 0.59 +0.5 3,846,133 + uhpC b3667 0.61 +1.1 3,846,171 + uhpC b3667 0.64 +0.5 3,846,171 + uhpC b3667 0.64 -0.1 3,846,176 - uhpC b3667 0.64 -1.9 3,846,176 - uhpC b3667 0.64 +0.1 3,846,236 - uhpC b3667 0.69 -0.9 3,846,271 + uhpC b3667 0.71 -0.8 3,846,271 + uhpC b3667 0.71 -0.6 3,846,284 - uhpC b3667 0.72 +0.4 3,846,344 + uhpC b3667 0.77 -0.6 3,846,344 + uhpC b3667 0.77 -0.0 3,846,347 - uhpC b3667 0.77 -1.8 3,846,410 - uhpC b3667 0.82 +3.4 3,846,411 + uhpC b3667 0.82 -0.9 3,846,418 - uhpC b3667 0.83 +1.4 3,846,419 - uhpC b3667 0.83 -0.0 3,846,429 - uhpC b3667 0.83 -0.6 3,846,429 - uhpC b3667 0.83 +0.6 3,846,429 - uhpC b3667 0.83 +0.3 3,846,429 - uhpC b3667 0.83 +1.1 3,846,554 - -0.6 3,846,604 + +0.7 3,846,655 + -0.6 3,846,674 - -1.1 3,846,674 - -1.6 3,846,685 - +0.4 3,846,689 + +0.7 3,846,815 - uhpB b3668 0.11 -2.5 3,846,815 - uhpB b3668 0.11 -1.7 3,846,965 + uhpB b3668 0.20 -2.0 3,846,965 + uhpB b3668 0.20 -1.1 3,846,983 - uhpB b3668 0.22 -1.6 3,846,983 - uhpB b3668 0.22 -0.1 3,847,125 + uhpB b3668 0.31 -2.3 3,847,125 + uhpB b3668 0.31 -1.4 3,847,172 - -0.6 3,847,174 + uhpB b3668 0.34 +0.1 3,847,190 + uhpB b3668 0.35 -0.9 3,847,203 + uhpB b3668 0.36 -0.8 3,847,243 - uhpB b3668 0.39 -1.8 3,847,253 - uhpB b3668 0.40 +0.2 3,847,264 - uhpB b3668 0.40 +3.1 3,847,327 + uhpB b3668 0.45 +1.4 3,847,370 + uhpB b3668 0.47 -2.0 3,847,422 - uhpB b3668 0.51 +2.0 3,847,422 - uhpB b3668 0.51 +0.2 3,847,464 + uhpB b3668 0.54 +0.6 3,847,480 + uhpB b3668 0.55 -0.6 3,847,488 - uhpB b3668 0.55 +0.2 3,847,494 - uhpB b3668 0.56 -0.1 3,847,516 + uhpB b3668 0.57 -1.1 3,847,593 + uhpB b3668 0.62 +1.7 3,847,593 + uhpB b3668 0.62 +2.4 3,847,665 - uhpB b3668 0.67 -0.7 3,847,665 - uhpB b3668 0.67 -1.4 3,847,723 - uhpB b3668 0.71 -1.4 3,847,815 + uhpB b3668 0.77 +1.2 3,847,815 + uhpB b3668 0.77 -1.4 3,847,825 - uhpB b3668 0.78 +0.2 3,847,825 - uhpB b3668 0.78 +1.3 3,847,828 - uhpB b3668 0.78 +0.4 3,847,828 - uhpB b3668 0.78 -1.1 3,847,880 - uhpB b3668 0.81 -0.6 3,847,883 - uhpB b3668 0.82 -0.6 3,847,883 - uhpB b3668 0.82 +0.7 3,847,942 + uhpB b3668 0.85 +0.1 3,848,011 - -1.1 3,848,016 - +1.6 3,848,029 - +1.1 3,848,029 - +2.7 3,848,043 + -1.6 3,848,045 + +0.1 3,848,047 + -1.6 3,848,074 - +0.7 3,848,074 - +0.1 3,848,083 - -1.1 3,848,094 - +2.2 3,848,129 + -0.5 3,848,129 + +2.6 3,848,163 - -0.6 3,848,165 + +1.4 3,848,193 + +0.3 3,848,193 + -1.1 3,848,196 - +0.0 3,848,237 + uhpA b3669 0.13 -1.2 3,848,250 - uhpA b3669 0.15 -1.0 3,848,261 + uhpA b3669 0.17 -2.0 3,848,285 - uhpA b3669 0.21 -2.6 3,848,370 - uhpA b3669 0.36 +1.4 3,848,439 + uhpA b3669 0.47 -1.6 3,848,492 - uhpA b3669 0.56 +1.4 3,848,492 - uhpA b3669 0.56 -0.6 3,848,494 + uhpA b3669 0.57 +0.1 3,848,494 + uhpA b3669 0.57 -1.6 3,848,494 + uhpA b3669 0.57 -0.4 3,848,494 + uhpA b3669 0.57 +1.1 3,848,504 + uhpA b3669 0.58 +0.3 3,848,504 + uhpA b3669 0.58 +1.4 3,848,524 - uhpA b3669 0.62 +1.4 3,848,524 - uhpA b3669 0.62 -1.6 3,848,524 - uhpA b3669 0.62 -1.1 3,848,526 - uhpA b3669 0.62 -2.6 3,848,565 - uhpA b3669 0.69 -0.1 3,848,565 - uhpA b3669 0.69 -1.1 3,848,571 + uhpA b3669 0.70 -0.5 3,848,571 + uhpA b3669 0.70 +0.9 3,848,577 + uhpA b3669 0.71 +0.1 3,848,585 - uhpA b3669 0.72 +0.5 3,848,595 + uhpA b3669 0.74 -3.3 3,848,603 - uhpA b3669 0.75 +2.6 3,848,608 - uhpA b3669 0.76 -0.7 3,848,610 - uhpA b3669 0.76 -0.4 3,848,719 + -0.1 3,848,734 + -0.5 3,848,904 - ilvN b3670 0.27 -0.1 3,848,921 + ilvN b3670 0.33 -2.6 3,848,950 + ilvN b3670 0.43 +0.0 3,848,953 + ilvN b3670 0.44 +1.2 3,848,953 + ilvN b3670 0.44 -0.9 3,848,958 - ilvN b3670 0.46 +3.0 3,848,958 - ilvN b3670 0.46 -1.5 3,848,966 + ilvN b3670 0.48 +0.5 3,848,978 + ilvN b3670 0.53 +1.0 3,848,978 + ilvN b3670 0.53 -0.7 3,848,986 - ilvN b3670 0.55 -0.6 3,849,000 - ilvN b3670 0.60 +0.4 3,849,000 - ilvN b3670 0.60 +1.4 3,849,044 - ilvN b3670 0.75 +0.1 3,849,044 - ilvN b3670 0.75 -0.7 3,849,044 - ilvN b3670 0.75 +1.0 3,849,050 + ilvN b3670 0.77 -0.4 3,849,050 + ilvN b3670 0.77 -1.1 3,849,055 - ilvN b3670 0.79 -1.3 3,849,103 - +1.9 3,849,103 - +1.9 3,849,110 - +0.9 3,849,140 - -0.7
Or see this region's nucleotide sequence