Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mutM and rpmG are separated by 97 nucleotides rpmG and rpmB are separated by 20 nucleotides rpmB and radC are separated by 216 nucleotides radC and dfp are separated by 171 nucleotides
b3635: mutM - formamidopyrimidine-DNA glycosylase (NCBI), at 3,808,366 to 3,809,175
mutM
b3636: rpmG - 50S ribosomal protein L33 (NCBI), at 3,809,273 to 3,809,440
rpmG
b3637: rpmB - 50S ribosomal protein L28 (NCBI), at 3,809,461 to 3,809,697
rpmB
b3638: radC - DNA repair protein (VIMSS), at 3,809,914 to 3,810,582
radC
b3639: dfp - flavoprotein affecting synthesis of DNA and pantothenate metabolism (VIMSS), at 3,810,754 to 3,811,974
dfp
Position (kb)
3809
3810
3811 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3808.931 kb on + strand, within mutM at 3808.931 kb on + strand, within mutM at 3808.954 kb on - strand, within mutM at 3808.954 kb on - strand, within mutM at 3808.954 kb on - strand, within mutM at 3808.987 kb on - strand, within mutM at 3809.014 kb on + strand, within mutM at 3809.014 kb on + strand, within mutM at 3809.031 kb on - strand, within mutM at 3809.056 kb on - strand, within mutM at 3809.056 kb on - strand, within mutM at 3809.066 kb on - strand, within mutM at 3809.105 kb on - strand at 3809.112 kb on - strand at 3809.114 kb on + strand at 3809.114 kb on + strand at 3809.130 kb on + strand at 3809.138 kb on - strand at 3809.138 kb on - strand at 3809.143 kb on - strand at 3809.177 kb on + strand at 3809.204 kb on + strand at 3809.204 kb on + strand at 3809.204 kb on + strand at 3809.206 kb on + strand at 3809.214 kb on - strand at 3809.214 kb on - strand at 3809.306 kb on - strand, within rpmG at 3809.306 kb on - strand, within rpmG at 3809.384 kb on - strand, within rpmG at 3809.432 kb on - strand at 3809.790 kb on - strand at 3809.851 kb on - strand at 3809.851 kb on - strand at 3809.852 kb on - strand at 3809.867 kb on + strand at 3810.048 kb on + strand, within radC at 3810.048 kb on + strand, within radC at 3810.053 kb on - strand, within radC at 3810.053 kb on - strand, within radC at 3810.053 kb on - strand, within radC at 3810.062 kb on + strand, within radC at 3810.063 kb on + strand, within radC at 3810.072 kb on + strand, within radC at 3810.080 kb on - strand, within radC at 3810.080 kb on - strand, within radC at 3810.087 kb on - strand, within radC at 3810.087 kb on - strand, within radC at 3810.087 kb on - strand, within radC at 3810.267 kb on + strand, within radC at 3810.267 kb on + strand, within radC at 3810.285 kb on - strand, within radC at 3810.317 kb on + strand, within radC at 3810.338 kb on + strand, within radC at 3810.346 kb on - strand, within radC at 3810.369 kb on + strand, within radC at 3810.462 kb on - strand, within radC at 3810.464 kb on + strand, within radC at 3810.464 kb on + strand, within radC at 3810.470 kb on - strand, within radC at 3810.482 kb on - strand, within radC at 3810.555 kb on + strand at 3810.564 kb on + strand at 3810.619 kb on + strand at 3810.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,808,931 + mutM b3635 0.70 -1.6 3,808,931 + mutM b3635 0.70 +1.7 3,808,954 - mutM b3635 0.73 +1.4 3,808,954 - mutM b3635 0.73 +1.1 3,808,954 - mutM b3635 0.73 +2.1 3,808,987 - mutM b3635 0.77 -0.6 3,809,014 + mutM b3635 0.80 +0.8 3,809,014 + mutM b3635 0.80 -1.8 3,809,031 - mutM b3635 0.82 -0.4 3,809,056 - mutM b3635 0.85 +0.8 3,809,056 - mutM b3635 0.85 -0.9 3,809,066 - mutM b3635 0.86 -1.6 3,809,105 - +1.8 3,809,112 - +1.4 3,809,114 + -0.8 3,809,114 + +1.9 3,809,130 + +1.1 3,809,138 - -2.9 3,809,138 - +1.5 3,809,143 - +0.1 3,809,177 + +2.6 3,809,204 + +3.6 3,809,204 + +0.8 3,809,204 + -0.4 3,809,206 + -1.3 3,809,214 - -1.8 3,809,214 - -0.0 3,809,306 - rpmG b3636 0.20 +0.4 3,809,306 - rpmG b3636 0.20 +1.4 3,809,384 - rpmG b3636 0.66 -1.1 3,809,432 - +1.4 3,809,790 - -0.2 3,809,851 - +0.4 3,809,851 - -2.3 3,809,852 - +2.4 3,809,867 + -0.6 3,810,048 + radC b3638 0.20 +1.3 3,810,048 + radC b3638 0.20 +1.3 3,810,053 - radC b3638 0.21 +2.4 3,810,053 - radC b3638 0.21 -0.3 3,810,053 - radC b3638 0.21 -1.9 3,810,062 + radC b3638 0.22 +2.0 3,810,063 + radC b3638 0.22 -0.2 3,810,072 + radC b3638 0.24 -0.8 3,810,080 - radC b3638 0.25 +2.7 3,810,080 - radC b3638 0.25 +1.6 3,810,087 - radC b3638 0.26 -0.6 3,810,087 - radC b3638 0.26 +0.0 3,810,087 - radC b3638 0.26 +1.3 3,810,267 + radC b3638 0.53 +3.3 3,810,267 + radC b3638 0.53 +3.0 3,810,285 - radC b3638 0.55 -2.7 3,810,317 + radC b3638 0.60 +1.3 3,810,338 + radC b3638 0.63 +0.4 3,810,346 - radC b3638 0.65 +0.5 3,810,369 + radC b3638 0.68 +0.7 3,810,462 - radC b3638 0.82 -0.7 3,810,464 + radC b3638 0.82 -0.5 3,810,464 + radC b3638 0.82 +1.0 3,810,470 - radC b3638 0.83 +2.1 3,810,482 - radC b3638 0.85 +0.0 3,810,555 + -1.4 3,810,564 + -1.9 3,810,619 + -1.3 3,810,729 - +0.8
Or see this region's nucleotide sequence