Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbbA and yhiI overlap by 4 nucleotides yhiI and yhiJ are separated by 365 nucleotides
b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561
rbbA
b3487: yhiI - predicted HlyD family secretion protein (NCBI), at 3,627,558 to 3,628,625
yhiI
b3488: yhiJ - hypothetical protein (NCBI), at 3,628,991 to 3,630,613
yhiJ
Position (kb)
3627
3628
3629 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3626.581 kb on + strand, within rbbA at 3626.617 kb on - strand, within rbbA at 3626.617 kb on - strand, within rbbA at 3626.720 kb on - strand, within rbbA at 3626.767 kb on + strand, within rbbA at 3626.771 kb on + strand, within rbbA at 3626.771 kb on + strand, within rbbA at 3626.775 kb on - strand, within rbbA at 3626.784 kb on - strand, within rbbA at 3626.889 kb on + strand, within rbbA at 3626.899 kb on + strand, within rbbA at 3626.899 kb on + strand, within rbbA at 3626.958 kb on + strand, within rbbA at 3626.979 kb on + strand, within rbbA at 3627.030 kb on + strand, within rbbA at 3627.060 kb on + strand, within rbbA at 3627.060 kb on + strand, within rbbA at 3627.072 kb on + strand, within rbbA at 3627.081 kb on - strand, within rbbA at 3627.081 kb on - strand, within rbbA at 3627.090 kb on + strand, within rbbA at 3627.090 kb on + strand, within rbbA at 3627.126 kb on - strand, within rbbA at 3627.166 kb on - strand, within rbbA at 3627.359 kb on - strand at 3627.366 kb on + strand at 3627.448 kb on - strand at 3627.448 kb on - strand at 3627.448 kb on - strand at 3627.449 kb on - strand at 3627.463 kb on - strand at 3627.581 kb on - strand at 3627.611 kb on + strand at 3627.611 kb on + strand at 3627.617 kb on + strand at 3627.617 kb on + strand at 3627.617 kb on + strand at 3627.660 kb on + strand at 3627.714 kb on - strand, within yhiI at 3627.750 kb on - strand, within yhiI at 3627.836 kb on + strand, within yhiI at 3627.854 kb on - strand, within yhiI at 3627.903 kb on + strand, within yhiI at 3627.906 kb on - strand, within yhiI at 3627.906 kb on - strand, within yhiI at 3627.938 kb on - strand, within yhiI at 3628.055 kb on - strand, within yhiI at 3628.063 kb on + strand, within yhiI at 3628.066 kb on - strand, within yhiI at 3628.199 kb on + strand, within yhiI at 3628.223 kb on + strand, within yhiI at 3628.223 kb on + strand, within yhiI at 3628.236 kb on + strand, within yhiI at 3628.244 kb on - strand, within yhiI at 3628.269 kb on - strand, within yhiI at 3628.303 kb on + strand, within yhiI at 3628.307 kb on - strand, within yhiI at 3628.307 kb on - strand, within yhiI at 3628.347 kb on - strand, within yhiI at 3628.365 kb on - strand, within yhiI at 3628.426 kb on - strand, within yhiI at 3628.426 kb on - strand, within yhiI at 3628.506 kb on + strand, within yhiI at 3628.617 kb on + strand at 3628.623 kb on - strand at 3628.671 kb on - strand at 3628.716 kb on - strand at 3628.745 kb on - strand at 3628.745 kb on - strand at 3628.749 kb on - strand at 3628.753 kb on + strand at 3628.753 kb on + strand at 3628.758 kb on + strand at 3628.769 kb on - strand at 3628.769 kb on - strand at 3628.776 kb on - strand at 3628.789 kb on + strand at 3628.792 kb on - strand at 3628.828 kb on - strand at 3628.843 kb on - strand at 3628.848 kb on + strand at 3628.882 kb on + strand at 3628.916 kb on - strand at 3628.952 kb on + strand at 3628.956 kb on - strand at 3628.997 kb on - strand at 3629.004 kb on - strand at 3629.068 kb on + strand at 3629.073 kb on + strand at 3629.075 kb on + strand at 3629.083 kb on - strand at 3629.086 kb on + strand at 3629.087 kb on - strand at 3629.089 kb on - strand at 3629.095 kb on - strand at 3629.095 kb on - strand at 3629.097 kb on - strand at 3629.130 kb on + strand at 3629.153 kb on - strand at 3629.162 kb on + strand, within yhiJ at 3629.162 kb on + strand, within yhiJ at 3629.162 kb on + strand, within yhiJ at 3629.162 kb on + strand, within yhiJ at 3629.162 kb on + strand, within yhiJ at 3629.180 kb on - strand, within yhiJ at 3629.184 kb on + strand, within yhiJ at 3629.189 kb on + strand, within yhiJ at 3629.191 kb on + strand, within yhiJ at 3629.194 kb on + strand, within yhiJ at 3629.194 kb on + strand, within yhiJ at 3629.213 kb on + strand, within yhiJ at 3629.213 kb on - strand, within yhiJ at 3629.215 kb on + strand, within yhiJ at 3629.215 kb on + strand, within yhiJ at 3629.227 kb on + strand, within yhiJ at 3629.227 kb on + strand, within yhiJ at 3629.242 kb on + strand, within yhiJ at 3629.242 kb on + strand, within yhiJ at 3629.242 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.252 kb on + strand, within yhiJ at 3629.260 kb on - strand, within yhiJ at 3629.269 kb on + strand, within yhiJ at 3629.269 kb on + strand, within yhiJ at 3629.275 kb on + strand, within yhiJ at 3629.326 kb on - strand, within yhiJ at 3629.359 kb on + strand, within yhiJ at 3629.404 kb on + strand, within yhiJ at 3629.404 kb on + strand, within yhiJ at 3629.425 kb on + strand, within yhiJ at 3629.430 kb on + strand, within yhiJ at 3629.446 kb on + strand, within yhiJ at 3629.446 kb on + strand, within yhiJ at 3629.454 kb on - strand, within yhiJ at 3629.561 kb on - strand, within yhiJ at 3629.591 kb on + strand, within yhiJ at 3629.599 kb on + strand, within yhiJ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,626,581 + rbbA b3486 0.64 +0.4 3,626,617 - rbbA b3486 0.65 +1.1 3,626,617 - rbbA b3486 0.65 -0.8 3,626,720 - rbbA b3486 0.69 +1.3 3,626,767 + rbbA b3486 0.71 -0.1 3,626,771 + rbbA b3486 0.71 -1.4 3,626,771 + rbbA b3486 0.71 -2.7 3,626,775 - rbbA b3486 0.71 -0.8 3,626,784 - rbbA b3486 0.72 -0.9 3,626,889 + rbbA b3486 0.75 +0.8 3,626,899 + rbbA b3486 0.76 +0.2 3,626,899 + rbbA b3486 0.76 -0.2 3,626,958 + rbbA b3486 0.78 +0.4 3,626,979 + rbbA b3486 0.79 +1.5 3,627,030 + rbbA b3486 0.81 +1.4 3,627,060 + rbbA b3486 0.82 +0.4 3,627,060 + rbbA b3486 0.82 -1.5 3,627,072 + rbbA b3486 0.82 -0.0 3,627,081 - rbbA b3486 0.82 -0.9 3,627,081 - rbbA b3486 0.82 -0.4 3,627,090 + rbbA b3486 0.83 -0.2 3,627,090 + rbbA b3486 0.83 +0.4 3,627,126 - rbbA b3486 0.84 -1.8 3,627,166 - rbbA b3486 0.86 -2.1 3,627,359 - -2.3 3,627,366 + +2.6 3,627,448 - +3.0 3,627,448 - -1.2 3,627,448 - -0.9 3,627,449 - +1.0 3,627,463 - +1.4 3,627,581 - -1.2 3,627,611 + -2.4 3,627,611 + -0.9 3,627,617 + +1.3 3,627,617 + +0.1 3,627,617 + -1.5 3,627,660 + -0.4 3,627,714 - yhiI b3487 0.15 -0.8 3,627,750 - yhiI b3487 0.18 +0.9 3,627,836 + yhiI b3487 0.26 -0.3 3,627,854 - yhiI b3487 0.28 -0.0 3,627,903 + yhiI b3487 0.32 -1.2 3,627,906 - yhiI b3487 0.33 +1.4 3,627,906 - yhiI b3487 0.33 +1.4 3,627,938 - yhiI b3487 0.36 +0.6 3,628,055 - yhiI b3487 0.47 -0.4 3,628,063 + yhiI b3487 0.47 -2.1 3,628,066 - yhiI b3487 0.48 -0.4 3,628,199 + yhiI b3487 0.60 +1.3 3,628,223 + yhiI b3487 0.62 -0.1 3,628,223 + yhiI b3487 0.62 -1.1 3,628,236 + yhiI b3487 0.63 -1.1 3,628,244 - yhiI b3487 0.64 +0.7 3,628,269 - yhiI b3487 0.67 -1.1 3,628,303 + yhiI b3487 0.70 -1.2 3,628,307 - yhiI b3487 0.70 +2.0 3,628,307 - yhiI b3487 0.70 +0.8 3,628,347 - yhiI b3487 0.74 -0.3 3,628,365 - yhiI b3487 0.76 -1.0 3,628,426 - yhiI b3487 0.81 +1.7 3,628,426 - yhiI b3487 0.81 -1.4 3,628,506 + yhiI b3487 0.89 -0.1 3,628,617 + -0.8 3,628,623 - -0.2 3,628,671 - +0.3 3,628,716 - -1.8 3,628,745 - -0.7 3,628,745 - -0.5 3,628,749 - -0.4 3,628,753 + +2.1 3,628,753 + +0.1 3,628,758 + +0.1 3,628,769 - +0.6 3,628,769 - -1.3 3,628,776 - -1.1 3,628,789 + -1.4 3,628,792 - -0.7 3,628,828 - -0.0 3,628,843 - +1.0 3,628,848 + -1.1 3,628,882 + +0.6 3,628,916 - -0.6 3,628,952 + -0.3 3,628,956 - -0.8 3,628,997 - -1.6 3,629,004 - +0.2 3,629,068 + -1.6 3,629,073 + +0.0 3,629,075 + +0.5 3,629,083 - -0.9 3,629,086 + -0.3 3,629,087 - -3.1 3,629,089 - +0.6 3,629,095 - -0.2 3,629,095 - +0.4 3,629,097 - +2.0 3,629,130 + +0.4 3,629,153 - -0.4 3,629,162 + yhiJ b3488 0.11 +1.1 3,629,162 + yhiJ b3488 0.11 +2.5 3,629,162 + yhiJ b3488 0.11 -1.6 3,629,162 + yhiJ b3488 0.11 -2.3 3,629,162 + yhiJ b3488 0.11 +0.5 3,629,180 - yhiJ b3488 0.12 +1.9 3,629,184 + yhiJ b3488 0.12 +0.7 3,629,189 + yhiJ b3488 0.12 -0.3 3,629,191 + yhiJ b3488 0.12 -0.9 3,629,194 + yhiJ b3488 0.13 +0.2 3,629,194 + yhiJ b3488 0.13 -0.4 3,629,213 + yhiJ b3488 0.14 +0.9 3,629,213 - yhiJ b3488 0.14 -0.2 3,629,215 + yhiJ b3488 0.14 +0.1 3,629,215 + yhiJ b3488 0.14 -1.4 3,629,227 + yhiJ b3488 0.15 +0.9 3,629,227 + yhiJ b3488 0.15 +0.6 3,629,242 + yhiJ b3488 0.15 +1.1 3,629,242 + yhiJ b3488 0.15 -2.1 3,629,242 + yhiJ b3488 0.15 -0.6 3,629,252 + yhiJ b3488 0.16 +2.0 3,629,252 + yhiJ b3488 0.16 +0.0 3,629,252 + yhiJ b3488 0.16 +0.9 3,629,252 + yhiJ b3488 0.16 +0.3 3,629,252 + yhiJ b3488 0.16 +0.1 3,629,252 + yhiJ b3488 0.16 -1.1 3,629,252 + yhiJ b3488 0.16 -1.9 3,629,260 - yhiJ b3488 0.17 -0.1 3,629,269 + yhiJ b3488 0.17 -3.1 3,629,269 + yhiJ b3488 0.17 +0.2 3,629,275 + yhiJ b3488 0.17 -0.8 3,629,326 - yhiJ b3488 0.21 -2.1 3,629,359 + yhiJ b3488 0.23 -0.6 3,629,404 + yhiJ b3488 0.25 +1.5 3,629,404 + yhiJ b3488 0.25 +1.8 3,629,425 + yhiJ b3488 0.27 +2.1 3,629,430 + yhiJ b3488 0.27 +1.6 3,629,446 + yhiJ b3488 0.28 -0.9 3,629,446 + yhiJ b3488 0.28 +0.2 3,629,454 - yhiJ b3488 0.29 -2.9 3,629,561 - yhiJ b3488 0.35 -0.5 3,629,591 + yhiJ b3488 0.37 -1.4 3,629,599 + yhiJ b3488 0.37 +1.5
Or see this region's nucleotide sequence