Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhI and yhhJ are separated by 164 nucleotides yhhJ and rbbA overlap by 1 nucleotides
b3484: yhhI - predicted transposase (NCBI), at 3,622,401 to 3,623,537
yhhI
b3485: yhhJ - predicted transporter subunit: membrane component of ABC superfamily (RefSeq), at 3,623,702 to 3,624,826
yhhJ
b3486: rbbA - fused ribosome-associated ATPase: ATP-binding protein/ATP-binding protein/predicted membrane protein (NCBI), at 3,624,826 to 3,627,561
rbbA
Position (kb)
3623
3624
3625 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3623.245 kb on + strand, within yhhI at 3623.298 kb on - strand, within yhhI at 3623.298 kb on - strand, within yhhI at 3623.318 kb on - strand, within yhhI at 3623.325 kb on - strand, within yhhI at 3623.337 kb on - strand, within yhhI at 3623.337 kb on - strand, within yhhI at 3623.340 kb on - strand, within yhhI at 3623.542 kb on + strand at 3623.679 kb on - strand at 3623.679 kb on - strand at 3623.681 kb on - strand at 3623.681 kb on - strand at 3623.733 kb on - strand at 3623.841 kb on + strand, within yhhJ at 3623.864 kb on + strand, within yhhJ at 3623.869 kb on + strand, within yhhJ at 3623.874 kb on + strand, within yhhJ at 3623.874 kb on + strand, within yhhJ at 3623.876 kb on + strand, within yhhJ at 3623.906 kb on - strand, within yhhJ at 3623.916 kb on - strand, within yhhJ at 3623.916 kb on - strand, within yhhJ at 3623.969 kb on - strand, within yhhJ at 3624.083 kb on + strand, within yhhJ at 3624.085 kb on + strand, within yhhJ at 3624.112 kb on + strand, within yhhJ at 3624.130 kb on + strand, within yhhJ at 3624.200 kb on + strand, within yhhJ at 3624.215 kb on + strand, within yhhJ at 3624.235 kb on + strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.238 kb on - strand, within yhhJ at 3624.256 kb on - strand, within yhhJ at 3624.256 kb on - strand, within yhhJ at 3624.273 kb on - strand, within yhhJ at 3624.317 kb on - strand, within yhhJ at 3624.317 kb on - strand, within yhhJ at 3624.365 kb on + strand, within yhhJ at 3624.407 kb on + strand, within yhhJ at 3624.407 kb on + strand, within yhhJ at 3624.448 kb on - strand, within yhhJ at 3624.489 kb on + strand, within yhhJ at 3624.539 kb on + strand, within yhhJ at 3624.566 kb on - strand, within yhhJ at 3624.566 kb on - strand, within yhhJ at 3624.612 kb on + strand, within yhhJ at 3624.612 kb on + strand, within yhhJ at 3624.683 kb on + strand, within yhhJ at 3624.779 kb on + strand at 3624.878 kb on - strand at 3624.878 kb on - strand at 3624.968 kb on - strand at 3624.968 kb on - strand at 3624.996 kb on + strand at 3624.997 kb on - strand at 3625.130 kb on - strand, within rbbA at 3625.142 kb on + strand, within rbbA at 3625.249 kb on - strand, within rbbA at 3625.264 kb on - strand, within rbbA at 3625.264 kb on - strand, within rbbA at 3625.304 kb on - strand, within rbbA at 3625.304 kb on - strand, within rbbA at 3625.319 kb on + strand, within rbbA at 3625.420 kb on - strand, within rbbA at 3625.420 kb on - strand, within rbbA at 3625.517 kb on + strand, within rbbA at 3625.517 kb on + strand, within rbbA at 3625.520 kb on - strand, within rbbA at 3625.525 kb on + strand, within rbbA at 3625.525 kb on - strand, within rbbA at 3625.528 kb on + strand, within rbbA at 3625.530 kb on - strand, within rbbA at 3625.530 kb on - strand, within rbbA at 3625.533 kb on - strand, within rbbA at 3625.533 kb on - strand, within rbbA at 3625.567 kb on + strand, within rbbA at 3625.678 kb on + strand, within rbbA at 3625.736 kb on + strand, within rbbA at 3625.736 kb on + strand, within rbbA at 3625.736 kb on + strand, within rbbA at 3625.754 kb on + strand, within rbbA at 3625.779 kb on + strand, within rbbA at 3625.779 kb on + strand, within rbbA at 3625.794 kb on - strand, within rbbA at 3625.794 kb on - strand, within rbbA at 3625.802 kb on - strand, within rbbA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,623,245 + yhhI b3484 0.74 +0.8 3,623,298 - yhhI b3484 0.79 +3.8 3,623,298 - yhhI b3484 0.79 -0.6 3,623,318 - yhhI b3484 0.81 -0.5 3,623,325 - yhhI b3484 0.81 +0.0 3,623,337 - yhhI b3484 0.82 +0.0 3,623,337 - yhhI b3484 0.82 -1.4 3,623,340 - yhhI b3484 0.83 -0.4 3,623,542 + -0.7 3,623,679 - +0.2 3,623,679 - -1.8 3,623,681 - +1.2 3,623,681 - -2.6 3,623,733 - +1.0 3,623,841 + yhhJ b3485 0.12 -0.2 3,623,864 + yhhJ b3485 0.14 -0.7 3,623,869 + yhhJ b3485 0.15 +0.4 3,623,874 + yhhJ b3485 0.15 -1.9 3,623,874 + yhhJ b3485 0.15 +0.1 3,623,876 + yhhJ b3485 0.15 -0.1 3,623,906 - yhhJ b3485 0.18 -0.8 3,623,916 - yhhJ b3485 0.19 +1.8 3,623,916 - yhhJ b3485 0.19 +0.8 3,623,969 - yhhJ b3485 0.24 -1.1 3,624,083 + yhhJ b3485 0.34 -0.9 3,624,085 + yhhJ b3485 0.34 +0.5 3,624,112 + yhhJ b3485 0.36 +3.0 3,624,130 + yhhJ b3485 0.38 -1.5 3,624,200 + yhhJ b3485 0.44 +1.9 3,624,215 + yhhJ b3485 0.46 -2.3 3,624,235 + yhhJ b3485 0.47 -0.2 3,624,238 - yhhJ b3485 0.48 -3.3 3,624,238 - yhhJ b3485 0.48 -0.6 3,624,238 - yhhJ b3485 0.48 -0.9 3,624,256 - yhhJ b3485 0.49 +0.4 3,624,256 - yhhJ b3485 0.49 +0.2 3,624,273 - yhhJ b3485 0.51 +1.6 3,624,317 - yhhJ b3485 0.55 +2.4 3,624,317 - yhhJ b3485 0.55 -2.2 3,624,365 + yhhJ b3485 0.59 -1.2 3,624,407 + yhhJ b3485 0.63 +0.3 3,624,407 + yhhJ b3485 0.63 -0.9 3,624,448 - yhhJ b3485 0.66 -1.5 3,624,489 + yhhJ b3485 0.70 +0.7 3,624,539 + yhhJ b3485 0.74 -0.6 3,624,566 - yhhJ b3485 0.77 -1.2 3,624,566 - yhhJ b3485 0.77 -0.9 3,624,612 + yhhJ b3485 0.81 +0.4 3,624,612 + yhhJ b3485 0.81 +2.4 3,624,683 + yhhJ b3485 0.87 -0.6 3,624,779 + -0.1 3,624,878 - +1.8 3,624,878 - +0.6 3,624,968 - +1.6 3,624,968 - +2.4 3,624,996 + +0.1 3,624,997 - +1.8 3,625,130 - rbbA b3486 0.11 +1.3 3,625,142 + rbbA b3486 0.12 +0.2 3,625,249 - rbbA b3486 0.15 -0.5 3,625,264 - rbbA b3486 0.16 +3.4 3,625,264 - rbbA b3486 0.16 +0.1 3,625,304 - rbbA b3486 0.17 -1.9 3,625,304 - rbbA b3486 0.17 +1.8 3,625,319 + rbbA b3486 0.18 +0.2 3,625,420 - rbbA b3486 0.22 +0.6 3,625,420 - rbbA b3486 0.22 +0.2 3,625,517 + rbbA b3486 0.25 -1.9 3,625,517 + rbbA b3486 0.25 -0.9 3,625,520 - rbbA b3486 0.25 -1.0 3,625,525 + rbbA b3486 0.26 +2.1 3,625,525 - rbbA b3486 0.26 -0.0 3,625,528 + rbbA b3486 0.26 +0.4 3,625,530 - rbbA b3486 0.26 -0.1 3,625,530 - rbbA b3486 0.26 +1.3 3,625,533 - rbbA b3486 0.26 -1.4 3,625,533 - rbbA b3486 0.26 -1.4 3,625,567 + rbbA b3486 0.27 +1.5 3,625,678 + rbbA b3486 0.31 -1.4 3,625,736 + rbbA b3486 0.33 +1.0 3,625,736 + rbbA b3486 0.33 -2.2 3,625,736 + rbbA b3486 0.33 -0.6 3,625,754 + rbbA b3486 0.34 -0.4 3,625,779 + rbbA b3486 0.35 -0.9 3,625,779 + rbbA b3486 0.35 -1.8 3,625,794 - rbbA b3486 0.35 -1.9 3,625,794 - rbbA b3486 0.35 +0.2 3,625,802 - rbbA b3486 0.36 +0.6
Or see this region's nucleotide sequence