Strain Fitness in Escherichia coli BW25113 around b3422

Experiment: Cisplatin 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrtcB and rtcR are separated by 188 nucleotidesrtcR and glpR overlap by 19 nucleotidesglpR and glpG are separated by 16 nucleotides b3421: rtcB - hypothetical protein (NCBI), at 3,554,875 to 3,556,101 rtcB b3422: rtcR - sigma 54-dependent transcriptional regulator of rtcBA expression (NCBI), at 3,556,290 to 3,557,888 rtcR b3423: glpR - DNA-binding transcriptional repressor (NCBI), at 3,557,870 to 3,558,628 glpR b3424: glpG - predicted intramembrane serine protease (NCBI), at 3,558,645 to 3,559,475 glpG Position (kb) 3556 3557 3558Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3555.394 kb on - strand, within rtcBat 3555.398 kb on + strand, within rtcBat 3555.428 kb on + strand, within rtcBat 3555.428 kb on - strand, within rtcBat 3555.428 kb on - strand, within rtcBat 3555.437 kb on + strand, within rtcBat 3555.447 kb on - strand, within rtcBat 3555.465 kb on + strand, within rtcBat 3555.535 kb on + strand, within rtcBat 3555.552 kb on + strand, within rtcBat 3555.554 kb on + strand, within rtcBat 3555.565 kb on - strand, within rtcBat 3555.565 kb on - strand, within rtcBat 3555.581 kb on + strand, within rtcBat 3555.591 kb on + strand, within rtcBat 3555.591 kb on + strand, within rtcBat 3555.714 kb on + strand, within rtcBat 3555.720 kb on - strand, within rtcBat 3555.749 kb on + strand, within rtcBat 3555.749 kb on + strand, within rtcBat 3555.826 kb on - strand, within rtcBat 3555.826 kb on - strand, within rtcBat 3555.889 kb on - strand, within rtcBat 3555.889 kb on - strand, within rtcBat 3555.926 kb on - strand, within rtcBat 3555.928 kb on + strand, within rtcBat 3555.952 kb on + strand, within rtcBat 3555.957 kb on + strand, within rtcBat 3555.957 kb on + strand, within rtcBat 3555.959 kb on - strand, within rtcBat 3555.959 kb on - strand, within rtcBat 3555.969 kb on - strand, within rtcBat 3555.970 kb on - strand, within rtcBat 3556.030 kb on + strandat 3556.031 kb on + strandat 3556.035 kb on + strandat 3556.035 kb on + strandat 3556.037 kb on + strandat 3556.043 kb on - strandat 3556.043 kb on - strandat 3556.092 kb on - strandat 3556.232 kb on - strandat 3556.232 kb on - strandat 3556.273 kb on + strandat 3556.292 kb on + strandat 3556.292 kb on + strandat 3556.297 kb on + strandat 3556.316 kb on + strandat 3556.326 kb on + strandat 3556.326 kb on + strandat 3556.326 kb on + strandat 3556.326 kb on + strandat 3556.326 kb on + strandat 3556.326 kb on + strandat 3556.334 kb on - strandat 3556.334 kb on - strandat 3556.334 kb on - strandat 3556.334 kb on - strandat 3556.334 kb on - strandat 3556.334 kb on - strandat 3556.358 kb on - strandat 3556.358 kb on - strandat 3556.372 kb on + strandat 3556.372 kb on + strandat 3556.380 kb on - strandat 3556.385 kb on - strandat 3556.392 kb on - strandat 3556.426 kb on - strandat 3556.548 kb on - strand, within rtcRat 3556.551 kb on + strand, within rtcRat 3556.731 kb on - strand, within rtcRat 3556.746 kb on - strand, within rtcRat 3556.746 kb on - strand, within rtcRat 3556.858 kb on + strand, within rtcRat 3556.858 kb on + strand, within rtcRat 3556.859 kb on + strand, within rtcRat 3556.933 kb on - strand, within rtcRat 3556.940 kb on - strand, within rtcRat 3556.956 kb on + strand, within rtcRat 3556.973 kb on - strand, within rtcRat 3556.973 kb on - strand, within rtcRat 3556.986 kb on - strand, within rtcRat 3557.003 kb on - strand, within rtcRat 3557.008 kb on - strand, within rtcRat 3557.019 kb on + strand, within rtcRat 3557.029 kb on + strand, within rtcRat 3557.034 kb on + strand, within rtcRat 3557.042 kb on - strand, within rtcRat 3557.042 kb on - strand, within rtcRat 3557.166 kb on + strand, within rtcRat 3557.179 kb on - strand, within rtcRat 3557.179 kb on - strand, within rtcRat 3557.218 kb on + strand, within rtcRat 3557.246 kb on + strand, within rtcRat 3557.246 kb on + strand, within rtcRat 3557.280 kb on + strand, within rtcRat 3557.345 kb on + strand, within rtcRat 3557.345 kb on + strand, within rtcRat 3557.347 kb on - strand, within rtcRat 3557.347 kb on - strand, within rtcRat 3557.368 kb on + strand, within rtcRat 3557.389 kb on + strand, within rtcRat 3557.506 kb on - strand, within rtcRat 3557.560 kb on - strand, within rtcRat 3557.577 kb on - strand, within rtcRat 3557.577 kb on - strand, within rtcRat 3557.583 kb on + strand, within rtcRat 3557.591 kb on - strand, within rtcRat 3557.603 kb on - strand, within rtcRat 3557.621 kb on + strand, within rtcRat 3557.673 kb on + strand, within rtcRat 3557.709 kb on - strand, within rtcRat 3557.709 kb on - strand, within rtcRat 3557.833 kb on - strandat 3557.849 kb on + strandat 3557.849 kb on + strandat 3557.881 kb on + strandat 3557.884 kb on + strandat 3557.908 kb on - strandat 3557.908 kb on - strandat 3557.948 kb on - strand, within glpRat 3557.949 kb on + strand, within glpRat 3557.959 kb on + strand, within glpRat 3557.968 kb on + strand, within glpRat 3558.019 kb on + strand, within glpRat 3558.019 kb on + strand, within glpRat 3558.069 kb on + strand, within glpRat 3558.069 kb on + strand, within glpRat 3558.101 kb on + strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.103 kb on - strand, within glpRat 3558.104 kb on - strand, within glpRat 3558.104 kb on - strand, within glpRat 3558.115 kb on + strand, within glpRat 3558.143 kb on - strand, within glpRat 3558.156 kb on - strand, within glpRat 3558.156 kb on - strand, within glpRat 3558.221 kb on - strand, within glpRat 3558.266 kb on + strand, within glpRat 3558.301 kb on + strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.309 kb on - strand, within glpRat 3558.327 kb on + strand, within glpRat 3558.364 kb on - strand, within glpRat 3558.364 kb on - strand, within glpRat 3558.407 kb on + strand, within glpRat 3558.409 kb on + strand, within glpRat 3558.415 kb on + strand, within glpRat 3558.523 kb on - strand, within glpRat 3558.572 kb on - strandat 3558.578 kb on + strandat 3558.578 kb on + strandat 3558.588 kb on - strandat 3558.606 kb on + strandat 3558.687 kb on + strandat 3558.753 kb on + strand, within glpGat 3558.766 kb on + strand, within glpGat 3558.774 kb on - strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.776 kb on + strand, within glpGat 3558.791 kb on - strand, within glpGat 3558.794 kb on - strand, within glpGat 3558.816 kb on - strand, within glpGat 3558.851 kb on - strand, within glpGat 3558.855 kb on - strand, within glpGat 3558.867 kb on - strand, within glpGat 3558.867 kb on - strand, within glpG

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Per-strain Table

Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml
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3,555,394 - rtcB b3421 0.42 -0.2
3,555,398 + rtcB b3421 0.43 +1.7
3,555,428 + rtcB b3421 0.45 +0.8
3,555,428 - rtcB b3421 0.45 +1.4
3,555,428 - rtcB b3421 0.45 -0.5
3,555,437 + rtcB b3421 0.46 +0.4
3,555,447 - rtcB b3421 0.47 +1.4
3,555,465 + rtcB b3421 0.48 -1.1
3,555,535 + rtcB b3421 0.54 -0.2
3,555,552 + rtcB b3421 0.55 -1.5
3,555,554 + rtcB b3421 0.55 +1.5
3,555,565 - rtcB b3421 0.56 -0.7
3,555,565 - rtcB b3421 0.56 -0.4
3,555,581 + rtcB b3421 0.58 -0.9
3,555,591 + rtcB b3421 0.58 +0.8
3,555,591 + rtcB b3421 0.58 +0.8
3,555,714 + rtcB b3421 0.68 -0.2
3,555,720 - rtcB b3421 0.69 +0.8
3,555,749 + rtcB b3421 0.71 +0.8
3,555,749 + rtcB b3421 0.71 +0.5
3,555,826 - rtcB b3421 0.78 -1.1
3,555,826 - rtcB b3421 0.78 +3.0
3,555,889 - rtcB b3421 0.83 -0.2
3,555,889 - rtcB b3421 0.83 -0.8
3,555,926 - rtcB b3421 0.86 +1.1
3,555,928 + rtcB b3421 0.86 -1.5
3,555,952 + rtcB b3421 0.88 +0.9
3,555,957 + rtcB b3421 0.88 -0.6
3,555,957 + rtcB b3421 0.88 +0.1
3,555,959 - rtcB b3421 0.88 -0.7
3,555,959 - rtcB b3421 0.88 -2.7
3,555,969 - rtcB b3421 0.89 -0.3
3,555,970 - rtcB b3421 0.89 +1.2
3,556,030 + +0.4
3,556,031 + -1.0
3,556,035 + +0.5
3,556,035 + -0.2
3,556,037 + +2.4
3,556,043 - +0.1
3,556,043 - -1.8
3,556,092 - +0.8
3,556,232 - -2.5
3,556,232 - -0.3
3,556,273 + -1.5
3,556,292 + -0.9
3,556,292 + +2.3
3,556,297 + +0.3
3,556,316 + +0.1
3,556,326 + -1.6
3,556,326 + +3.3
3,556,326 + -1.4
3,556,326 + -0.6
3,556,326 + +0.8
3,556,326 + +3.0
3,556,334 - +2.0
3,556,334 - -0.2
3,556,334 - +0.1
3,556,334 - -0.7
3,556,334 - +1.1
3,556,334 - +1.7
3,556,358 - -1.7
3,556,358 - -0.6
3,556,372 + +0.4
3,556,372 + -1.4
3,556,380 - +0.1
3,556,385 - +0.4
3,556,392 - -0.9
3,556,426 - +1.3
3,556,548 - rtcR b3422 0.16 -0.0
3,556,551 + rtcR b3422 0.16 +2.0
3,556,731 - rtcR b3422 0.28 +0.4
3,556,746 - rtcR b3422 0.29 +1.7
3,556,746 - rtcR b3422 0.29 -1.6
3,556,858 + rtcR b3422 0.36 -1.2
3,556,858 + rtcR b3422 0.36 -0.4
3,556,859 + rtcR b3422 0.36 -0.9
3,556,933 - rtcR b3422 0.40 +0.8
3,556,940 - rtcR b3422 0.41 +0.0
3,556,956 + rtcR b3422 0.42 +0.0
3,556,973 - rtcR b3422 0.43 +1.0
3,556,973 - rtcR b3422 0.43 +0.4
3,556,986 - rtcR b3422 0.44 +1.9
3,557,003 - rtcR b3422 0.45 +1.4
3,557,008 - rtcR b3422 0.45 +0.4
3,557,019 + rtcR b3422 0.46 +1.7
3,557,029 + rtcR b3422 0.46 +2.6
3,557,034 + rtcR b3422 0.47 -1.4
3,557,042 - rtcR b3422 0.47 -1.2
3,557,042 - rtcR b3422 0.47 +1.8
3,557,166 + rtcR b3422 0.55 -0.1
3,557,179 - rtcR b3422 0.56 -0.1
3,557,179 - rtcR b3422 0.56 +1.0
3,557,218 + rtcR b3422 0.58 +3.0
3,557,246 + rtcR b3422 0.60 +0.1
3,557,246 + rtcR b3422 0.60 -0.2
3,557,280 + rtcR b3422 0.62 -0.2
3,557,345 + rtcR b3422 0.66 +2.0
3,557,345 + rtcR b3422 0.66 -0.6
3,557,347 - rtcR b3422 0.66 +0.8
3,557,347 - rtcR b3422 0.66 -0.5
3,557,368 + rtcR b3422 0.67 -1.2
3,557,389 + rtcR b3422 0.69 -1.8
3,557,506 - rtcR b3422 0.76 +1.1
3,557,560 - rtcR b3422 0.79 -0.8
3,557,577 - rtcR b3422 0.80 +0.8
3,557,577 - rtcR b3422 0.80 -0.2
3,557,583 + rtcR b3422 0.81 -1.2
3,557,591 - rtcR b3422 0.81 +0.7
3,557,603 - rtcR b3422 0.82 +1.0
3,557,621 + rtcR b3422 0.83 +2.9
3,557,673 + rtcR b3422 0.86 -0.2
3,557,709 - rtcR b3422 0.89 +0.1
3,557,709 - rtcR b3422 0.89 -1.8
3,557,833 - -1.7
3,557,849 + -1.7
3,557,849 + -2.1
3,557,881 + -0.6
3,557,884 + -0.9
3,557,908 - +2.0
3,557,908 - +0.4
3,557,948 - glpR b3423 0.10 +0.8
3,557,949 + glpR b3423 0.10 +1.1
3,557,959 + glpR b3423 0.12 +0.1
3,557,968 + glpR b3423 0.13 -1.3
3,558,019 + glpR b3423 0.20 +0.3
3,558,019 + glpR b3423 0.20 -0.8
3,558,069 + glpR b3423 0.26 +1.0
3,558,069 + glpR b3423 0.26 -1.2
3,558,101 + glpR b3423 0.30 -0.4
3,558,103 - glpR b3423 0.31 -0.2
3,558,103 - glpR b3423 0.31 +0.8
3,558,103 - glpR b3423 0.31 -0.1
3,558,104 - glpR b3423 0.31 +0.6
3,558,104 - glpR b3423 0.31 +0.4
3,558,115 + glpR b3423 0.32 +0.1
3,558,143 - glpR b3423 0.36 +2.4
3,558,156 - glpR b3423 0.38 +1.8
3,558,156 - glpR b3423 0.38 +1.4
3,558,221 - glpR b3423 0.46 +1.1
3,558,266 + glpR b3423 0.52 +2.2
3,558,301 + glpR b3423 0.57 +2.6
3,558,309 - glpR b3423 0.58 -1.1
3,558,309 - glpR b3423 0.58 +0.5
3,558,309 - glpR b3423 0.58 -1.5
3,558,309 - glpR b3423 0.58 -0.2
3,558,309 - glpR b3423 0.58 +2.7
3,558,327 + glpR b3423 0.60 -1.0
3,558,364 - glpR b3423 0.65 -0.4
3,558,364 - glpR b3423 0.65 -0.6
3,558,407 + glpR b3423 0.71 +1.4
3,558,409 + glpR b3423 0.71 -0.6
3,558,415 + glpR b3423 0.72 -0.2
3,558,523 - glpR b3423 0.86 -0.4
3,558,572 - -0.1
3,558,578 + -1.9
3,558,578 + -0.2
3,558,588 - +2.2
3,558,606 + +0.2
3,558,687 + -0.5
3,558,753 + glpG b3424 0.13 -2.5
3,558,766 + glpG b3424 0.15 -0.9
3,558,774 - glpG b3424 0.16 +3.2
3,558,776 + glpG b3424 0.16 -3.1
3,558,776 + glpG b3424 0.16 -0.7
3,558,791 - glpG b3424 0.18 -0.7
3,558,794 - glpG b3424 0.18 -1.9
3,558,816 - glpG b3424 0.21 +1.4
3,558,851 - glpG b3424 0.25 -1.6
3,558,855 - glpG b3424 0.25 +0.2
3,558,867 - glpG b3424 0.27 -1.4
3,558,867 - glpG b3424 0.27 -0.2

Or see this region's nucleotide sequence