Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt feoB and yhgG overlap by 1 nucleotides yhgG and yhgA are separated by 202 nucleotides yhgA and bioH are separated by 28 nucleotides bioH and yhgH are separated by 37 nucleotides
b3409: feoB - ferrous iron transport protein B (NCBI), at 3,538,429 to 3,540,750
feoB
b3410: yhgG - predicted DNA-binding transcriptional regulator (NCBI), at 3,540,750 to 3,540,986
yhgG
b3411: yhgA - predicted transposase (NCBI), at 3,541,189 to 3,542,067
yhgA
b3412: bioH - carboxylesterase of pimeloyl-CoA synthesis (NCBI), at 3,542,096 to 3,542,866
bioH
b3413: yhgH - orf, hypothetical protein (VIMSS), at 3,542,904 to 3,543,587
yhgH
Position (kb)
3541
3542
3543 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3540.189 kb on + strand, within feoB at 3540.198 kb on + strand, within feoB at 3540.198 kb on + strand, within feoB at 3540.206 kb on - strand, within feoB at 3540.206 kb on - strand, within feoB at 3540.206 kb on - strand, within feoB at 3540.206 kb on - strand, within feoB at 3540.262 kb on + strand, within feoB at 3540.265 kb on - strand, within feoB at 3540.294 kb on - strand, within feoB at 3540.309 kb on + strand, within feoB at 3540.314 kb on + strand, within feoB at 3540.314 kb on + strand, within feoB at 3540.317 kb on + strand, within feoB at 3540.322 kb on - strand, within feoB at 3540.328 kb on - strand, within feoB at 3540.335 kb on - strand, within feoB at 3540.372 kb on - strand, within feoB at 3540.383 kb on + strand, within feoB at 3540.391 kb on - strand, within feoB at 3540.418 kb on + strand, within feoB at 3540.418 kb on + strand, within feoB at 3540.418 kb on + strand, within feoB at 3540.418 kb on + strand, within feoB at 3540.418 kb on + strand, within feoB at 3540.450 kb on - strand, within feoB at 3540.473 kb on + strand, within feoB at 3540.473 kb on + strand, within feoB at 3540.474 kb on - strand, within feoB at 3540.474 kb on - strand, within feoB at 3540.474 kb on - strand, within feoB at 3540.481 kb on - strand, within feoB at 3540.501 kb on + strand, within feoB at 3540.520 kb on - strand at 3540.603 kb on + strand at 3540.603 kb on + strand at 3540.619 kb on + strand at 3540.619 kb on + strand at 3540.633 kb on - strand at 3540.633 kb on - strand at 3540.640 kb on + strand at 3540.676 kb on - strand at 3540.679 kb on - strand at 3540.679 kb on - strand at 3540.796 kb on + strand, within yhgG at 3540.796 kb on + strand, within yhgG at 3540.801 kb on - strand, within yhgG at 3540.804 kb on - strand, within yhgG at 3540.804 kb on - strand, within yhgG at 3540.804 kb on - strand, within yhgG at 3540.824 kb on - strand, within yhgG at 3540.824 kb on - strand, within yhgG at 3540.839 kb on + strand, within yhgG at 3540.840 kb on - strand, within yhgG at 3540.840 kb on - strand, within yhgG at 3540.847 kb on - strand, within yhgG at 3540.847 kb on - strand, within yhgG at 3540.853 kb on - strand, within yhgG at 3540.853 kb on - strand, within yhgG at 3540.878 kb on + strand, within yhgG at 3540.878 kb on + strand, within yhgG at 3540.886 kb on - strand, within yhgG at 3540.886 kb on - strand, within yhgG at 3540.960 kb on + strand, within yhgG at 3540.960 kb on + strand, within yhgG at 3540.975 kb on - strand at 3540.975 kb on - strand at 3540.978 kb on - strand at 3540.980 kb on + strand at 3540.980 kb on + strand at 3540.988 kb on - strand at 3541.024 kb on + strand at 3541.070 kb on + strand at 3541.072 kb on + strand at 3541.091 kb on - strand at 3541.091 kb on - strand at 3541.103 kb on + strand at 3541.135 kb on + strand at 3541.136 kb on + strand at 3541.136 kb on + strand at 3541.136 kb on + strand at 3541.146 kb on - strand at 3541.153 kb on - strand at 3541.248 kb on + strand at 3541.266 kb on - strand at 3541.319 kb on + strand, within yhgA at 3541.333 kb on - strand, within yhgA at 3541.340 kb on + strand, within yhgA at 3541.340 kb on + strand, within yhgA at 3541.380 kb on + strand, within yhgA at 3541.380 kb on + strand, within yhgA at 3541.386 kb on - strand, within yhgA at 3541.408 kb on + strand, within yhgA at 3541.428 kb on + strand, within yhgA at 3541.440 kb on - strand, within yhgA at 3541.476 kb on + strand, within yhgA at 3541.484 kb on - strand, within yhgA at 3541.495 kb on + strand, within yhgA at 3541.572 kb on + strand, within yhgA at 3541.577 kb on + strand, within yhgA at 3541.577 kb on + strand, within yhgA at 3541.577 kb on + strand, within yhgA at 3541.581 kb on - strand, within yhgA at 3541.595 kb on - strand, within yhgA at 3541.595 kb on - strand, within yhgA at 3541.643 kb on + strand, within yhgA at 3541.681 kb on - strand, within yhgA at 3541.698 kb on + strand, within yhgA at 3541.701 kb on + strand, within yhgA at 3541.748 kb on + strand, within yhgA at 3541.757 kb on + strand, within yhgA at 3541.788 kb on - strand, within yhgA at 3541.798 kb on - strand, within yhgA at 3541.812 kb on + strand, within yhgA at 3541.812 kb on + strand, within yhgA at 3541.819 kb on + strand, within yhgA at 3541.913 kb on + strand, within yhgA at 3541.963 kb on + strand, within yhgA at 3541.968 kb on + strand, within yhgA at 3541.986 kb on - strand at 3541.986 kb on - strand at 3542.095 kb on + strand at 3542.103 kb on - strand at 3542.109 kb on - strand at 3542.115 kb on - strand at 3542.182 kb on - strand, within bioH at 3542.182 kb on - strand, within bioH at 3542.236 kb on + strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.244 kb on - strand, within bioH at 3542.361 kb on + strand, within bioH at 3542.361 kb on + strand, within bioH at 3542.366 kb on + strand, within bioH at 3542.405 kb on - strand, within bioH at 3542.441 kb on - strand, within bioH at 3542.576 kb on + strand, within bioH at 3542.576 kb on + strand, within bioH at 3542.580 kb on + strand, within bioH at 3542.584 kb on + strand, within bioH at 3542.593 kb on - strand, within bioH at 3542.692 kb on + strand, within bioH at 3542.741 kb on - strand, within bioH at 3542.741 kb on - strand, within bioH at 3542.767 kb on + strand, within bioH at 3542.767 kb on + strand, within bioH at 3542.775 kb on - strand, within bioH at 3542.775 kb on - strand, within bioH at 3542.785 kb on - strand, within bioH at 3542.797 kb on + strand at 3542.797 kb on + strand at 3542.800 kb on - strand at 3542.805 kb on - strand at 3542.805 kb on - strand at 3542.805 kb on - strand at 3542.812 kb on - strand at 3542.826 kb on + strand at 3542.830 kb on + strand at 3542.830 kb on + strand at 3542.893 kb on + strand at 3542.903 kb on + strand at 3542.903 kb on + strand at 3542.953 kb on - strand at 3542.953 kb on - strand at 3542.974 kb on - strand, within yhgH at 3542.974 kb on - strand, within yhgH at 3543.006 kb on + strand, within yhgH at 3543.028 kb on - strand, within yhgH
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,540,189 + feoB b3409 0.76 -0.9 3,540,198 + feoB b3409 0.76 -0.7 3,540,198 + feoB b3409 0.76 +2.6 3,540,206 - feoB b3409 0.77 -0.1 3,540,206 - feoB b3409 0.77 +1.1 3,540,206 - feoB b3409 0.77 +0.9 3,540,206 - feoB b3409 0.77 -0.0 3,540,262 + feoB b3409 0.79 +1.1 3,540,265 - feoB b3409 0.79 +2.6 3,540,294 - feoB b3409 0.80 +0.2 3,540,309 + feoB b3409 0.81 +0.5 3,540,314 + feoB b3409 0.81 -1.4 3,540,314 + feoB b3409 0.81 +0.8 3,540,317 + feoB b3409 0.81 +0.2 3,540,322 - feoB b3409 0.82 +1.0 3,540,328 - feoB b3409 0.82 +1.4 3,540,335 - feoB b3409 0.82 -0.7 3,540,372 - feoB b3409 0.84 -0.2 3,540,383 + feoB b3409 0.84 +0.4 3,540,391 - feoB b3409 0.84 +0.9 3,540,418 + feoB b3409 0.86 -0.1 3,540,418 + feoB b3409 0.86 -0.1 3,540,418 + feoB b3409 0.86 -0.6 3,540,418 + feoB b3409 0.86 -0.5 3,540,418 + feoB b3409 0.86 +1.0 3,540,450 - feoB b3409 0.87 +0.5 3,540,473 + feoB b3409 0.88 -2.5 3,540,473 + feoB b3409 0.88 +0.1 3,540,474 - feoB b3409 0.88 +1.4 3,540,474 - feoB b3409 0.88 -0.8 3,540,474 - feoB b3409 0.88 -1.3 3,540,481 - feoB b3409 0.88 -1.5 3,540,501 + feoB b3409 0.89 +1.1 3,540,520 - +1.9 3,540,603 + -0.2 3,540,603 + +2.2 3,540,619 + +0.8 3,540,619 + +1.4 3,540,633 - -1.4 3,540,633 - +2.1 3,540,640 + -0.0 3,540,676 - -1.8 3,540,679 - +0.3 3,540,679 - -0.8 3,540,796 + yhgG b3410 0.19 -1.1 3,540,796 + yhgG b3410 0.19 +0.4 3,540,801 - yhgG b3410 0.22 +0.2 3,540,804 - yhgG b3410 0.23 +0.9 3,540,804 - yhgG b3410 0.23 +0.9 3,540,804 - yhgG b3410 0.23 -0.2 3,540,824 - yhgG b3410 0.31 +0.8 3,540,824 - yhgG b3410 0.31 +0.4 3,540,839 + yhgG b3410 0.38 -0.3 3,540,840 - yhgG b3410 0.38 -2.3 3,540,840 - yhgG b3410 0.38 +1.0 3,540,847 - yhgG b3410 0.41 +1.4 3,540,847 - yhgG b3410 0.41 +4.9 3,540,853 - yhgG b3410 0.43 -1.9 3,540,853 - yhgG b3410 0.43 -1.8 3,540,878 + yhgG b3410 0.54 -0.9 3,540,878 + yhgG b3410 0.54 +1.4 3,540,886 - yhgG b3410 0.57 -0.2 3,540,886 - yhgG b3410 0.57 +1.4 3,540,960 + yhgG b3410 0.89 +0.9 3,540,960 + yhgG b3410 0.89 -0.7 3,540,975 - +1.8 3,540,975 - -1.1 3,540,978 - -1.9 3,540,980 + -1.6 3,540,980 + +0.4 3,540,988 - +0.8 3,541,024 + +2.0 3,541,070 + -0.4 3,541,072 + +0.2 3,541,091 - +0.8 3,541,091 - +0.3 3,541,103 + +1.6 3,541,135 + +1.4 3,541,136 + -0.3 3,541,136 + -0.8 3,541,136 + -0.6 3,541,146 - -0.5 3,541,153 - -0.7 3,541,248 + +1.3 3,541,266 - -1.8 3,541,319 + yhgA b3411 0.15 -0.1 3,541,333 - yhgA b3411 0.16 +0.5 3,541,340 + yhgA b3411 0.17 -1.2 3,541,340 + yhgA b3411 0.17 +0.6 3,541,380 + yhgA b3411 0.22 +1.2 3,541,380 + yhgA b3411 0.22 -1.2 3,541,386 - yhgA b3411 0.22 -1.2 3,541,408 + yhgA b3411 0.25 -0.8 3,541,428 + yhgA b3411 0.27 +1.9 3,541,440 - yhgA b3411 0.29 +2.0 3,541,476 + yhgA b3411 0.33 +0.3 3,541,484 - yhgA b3411 0.34 -0.9 3,541,495 + yhgA b3411 0.35 +1.9 3,541,572 + yhgA b3411 0.44 -0.6 3,541,577 + yhgA b3411 0.44 -0.6 3,541,577 + yhgA b3411 0.44 -0.8 3,541,577 + yhgA b3411 0.44 -1.6 3,541,581 - yhgA b3411 0.45 +3.2 3,541,595 - yhgA b3411 0.46 +0.8 3,541,595 - yhgA b3411 0.46 -0.1 3,541,643 + yhgA b3411 0.52 +0.4 3,541,681 - yhgA b3411 0.56 -0.1 3,541,698 + yhgA b3411 0.58 +0.5 3,541,701 + yhgA b3411 0.58 -0.2 3,541,748 + yhgA b3411 0.64 -0.8 3,541,757 + yhgA b3411 0.65 -0.2 3,541,788 - yhgA b3411 0.68 +1.8 3,541,798 - yhgA b3411 0.69 +0.6 3,541,812 + yhgA b3411 0.71 +2.3 3,541,812 + yhgA b3411 0.71 +0.5 3,541,819 + yhgA b3411 0.72 +0.9 3,541,913 + yhgA b3411 0.82 +2.5 3,541,963 + yhgA b3411 0.88 +0.3 3,541,968 + yhgA b3411 0.89 +0.1 3,541,986 - +1.1 3,541,986 - +1.0 3,542,095 + -1.1 3,542,103 - +1.1 3,542,109 - -0.9 3,542,115 - -0.4 3,542,182 - bioH b3412 0.11 +1.2 3,542,182 - bioH b3412 0.11 +0.4 3,542,236 + bioH b3412 0.18 +1.6 3,542,244 - bioH b3412 0.19 +0.1 3,542,244 - bioH b3412 0.19 -0.8 3,542,244 - bioH b3412 0.19 +1.0 3,542,244 - bioH b3412 0.19 +1.4 3,542,361 + bioH b3412 0.34 -0.2 3,542,361 + bioH b3412 0.34 -1.9 3,542,366 + bioH b3412 0.35 -2.5 3,542,405 - bioH b3412 0.40 -1.1 3,542,441 - bioH b3412 0.45 -0.2 3,542,576 + bioH b3412 0.62 -0.2 3,542,576 + bioH b3412 0.62 -0.8 3,542,580 + bioH b3412 0.63 +1.4 3,542,584 + bioH b3412 0.63 +0.7 3,542,593 - bioH b3412 0.64 -1.8 3,542,692 + bioH b3412 0.77 +0.4 3,542,741 - bioH b3412 0.84 -0.4 3,542,741 - bioH b3412 0.84 +1.5 3,542,767 + bioH b3412 0.87 +0.4 3,542,767 + bioH b3412 0.87 -0.2 3,542,775 - bioH b3412 0.88 -1.8 3,542,775 - bioH b3412 0.88 +0.6 3,542,785 - bioH b3412 0.89 -0.9 3,542,797 + +1.4 3,542,797 + -1.8 3,542,800 - -0.9 3,542,805 - -0.2 3,542,805 - +0.1 3,542,805 - +0.5 3,542,812 - -0.9 3,542,826 + -2.4 3,542,830 + +0.9 3,542,830 + +1.1 3,542,893 + -1.8 3,542,903 + +0.5 3,542,903 + +1.2 3,542,953 - +0.9 3,542,953 - -0.0 3,542,974 - yhgH b3413 0.10 -1.9 3,542,974 - yhgH b3413 0.10 -0.1 3,543,006 + yhgH b3413 0.15 +1.6 3,543,028 - yhgH b3413 0.18 -0.7
Or see this region's nucleotide sequence