Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt garD and sohA are separated by 148 nucleotides sohA and yhaV overlap by 1 nucleotides yhaV and agaR are separated by 54 nucleotides
b3128: garD - (D)-galactarate dehydrogenase (NCBI), at 3,273,304 to 3,274,875
garD
b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359
sohA
b3130: yhaV - hypothetical protein (NCBI), at 3,275,359 to 3,275,823
yhaV
b3131: agaR - DNA-binding transcriptional dual regulator (NCBI), at 3,275,878 to 3,276,687
agaR
Position (kb)
3275
3276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3274.468 kb on - strand, within garD at 3274.508 kb on - strand, within garD at 3274.541 kb on - strand, within garD at 3274.571 kb on + strand, within garD at 3274.619 kb on + strand, within garD at 3274.623 kb on + strand, within garD at 3274.709 kb on + strand, within garD at 3274.836 kb on - strand at 3275.046 kb on + strand at 3275.046 kb on + strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.051 kb on + strand at 3275.051 kb on + strand at 3275.057 kb on - strand at 3275.059 kb on - strand, within sohA at 3275.063 kb on + strand, within sohA at 3275.098 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.400 kb on - strand at 3275.400 kb on - strand at 3275.420 kb on - strand, within yhaV at 3275.427 kb on + strand, within yhaV at 3275.451 kb on + strand, within yhaV at 3275.512 kb on + strand, within yhaV at 3275.536 kb on + strand, within yhaV at 3275.596 kb on + strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.678 kb on + strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.690 kb on - strand, within yhaV at 3275.735 kb on + strand, within yhaV at 3275.741 kb on - strand, within yhaV at 3275.781 kb on - strand at 3275.781 kb on - strand at 3276.109 kb on + strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.170 kb on + strand, within agaR at 3276.242 kb on - strand, within agaR at 3276.242 kb on - strand at 3276.270 kb on + strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.560 kb on - strand, within agaR at 3276.602 kb on - strand, within agaR at 3276.625 kb on - strand at 3276.635 kb on - strand at 3276.643 kb on + strand at 3276.762 kb on - strand at 3276.790 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,274,468 - garD b3128 0.74 -0.1 3,274,508 - garD b3128 0.77 +0.9 3,274,541 - garD b3128 0.79 -2.3 3,274,571 + garD b3128 0.81 -0.6 3,274,619 + garD b3128 0.84 -0.7 3,274,623 + garD b3128 0.84 +2.2 3,274,709 + garD b3128 0.89 -1.6 3,274,836 - -0.1 3,275,046 + +0.1 3,275,046 + -0.9 3,275,049 - +1.0 3,275,049 - -0.8 3,275,049 - -1.1 3,275,051 + +1.6 3,275,051 + +1.4 3,275,057 - -0.9 3,275,059 - sohA b3129 0.10 -0.9 3,275,063 + sohA b3129 0.12 +0.4 3,275,098 + sohA b3129 0.22 -1.2 3,275,108 + sohA b3129 0.25 +0.2 3,275,108 + sohA b3129 0.25 +4.5 3,275,118 + sohA b3129 0.28 -0.2 3,275,118 + sohA b3129 0.28 -0.2 3,275,318 - sohA b3129 0.88 -0.2 3,275,318 - sohA b3129 0.88 +0.4 3,275,392 + -0.9 3,275,392 + +2.5 3,275,392 + -1.8 3,275,400 - -0.2 3,275,400 - +1.9 3,275,420 - yhaV b3130 0.13 +1.0 3,275,427 + yhaV b3130 0.15 -0.9 3,275,451 + yhaV b3130 0.20 -2.5 3,275,512 + yhaV b3130 0.33 -2.4 3,275,536 + yhaV b3130 0.38 +0.0 3,275,596 + yhaV b3130 0.51 +1.0 3,275,642 - yhaV b3130 0.61 -0.6 3,275,642 - yhaV b3130 0.61 -2.6 3,275,678 + yhaV b3130 0.69 +0.2 3,275,685 - yhaV b3130 0.70 +0.7 3,275,685 - yhaV b3130 0.70 -1.4 3,275,686 - yhaV b3130 0.70 +2.2 3,275,686 - yhaV b3130 0.70 +1.4 3,275,690 - yhaV b3130 0.71 -3.1 3,275,735 + yhaV b3130 0.81 +1.8 3,275,741 - yhaV b3130 0.82 -3.1 3,275,781 - +1.4 3,275,781 - -0.6 3,276,109 + agaR b3131 0.29 -1.2 3,276,131 - agaR b3131 0.31 +0.7 3,276,131 - agaR b3131 0.31 +0.2 3,276,131 - agaR b3131 0.31 +1.4 3,276,170 + agaR b3131 0.36 -1.3 3,276,242 - agaR b3131 0.45 +2.1 3,276,242 - +1.4 3,276,270 + agaR b3131 0.48 -1.2 3,276,447 - agaR b3131 0.70 +0.6 3,276,447 - agaR b3131 0.70 -0.9 3,276,560 - agaR b3131 0.84 -1.2 3,276,602 - agaR b3131 0.89 +1.6 3,276,625 - -2.0 3,276,635 - +0.7 3,276,643 + +0.2 3,276,762 - +2.8 3,276,790 - +2.0
Or see this region's nucleotide sequence