Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygjM and ygjN overlap by 4 nucleotides ygjN and ygjO are separated by 283 nucleotides ygjO and ygjP are separated by 84 nucleotides ygjP and ygjQ are separated by 76 nucleotides
b3082: ygjM - predicted DNA-binding transcriptional regulator (NCBI), at 3,231,750 to 3,232,166
ygjM
b3083: ygjN - hypothetical protein (NCBI), at 3,232,163 to 3,232,477
ygjN
b3084: ygjO - putative enzyme (VIMSS), at 3,232,761 to 3,233,897
ygjO
b3085: ygjP - orf, hypothetical protein (VIMSS), at 3,233,982 to 3,234,485
ygjP
b3086: ygjQ - predicted thioredoxin-like (NCBI), at 3,234,562 to 3,235,254
ygjQ
Position (kb)
3232
3233
3234 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3231.812 kb on - strand, within ygjM at 3231.855 kb on - strand, within ygjM at 3231.856 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3231.892 kb on - strand, within ygjM at 3232.179 kb on + strand at 3232.179 kb on + strand at 3232.181 kb on + strand at 3232.186 kb on + strand at 3232.189 kb on - strand at 3232.233 kb on + strand, within ygjN at 3232.267 kb on + strand, within ygjN at 3232.272 kb on - strand, within ygjN at 3232.302 kb on + strand, within ygjN at 3232.394 kb on + strand, within ygjN at 3232.416 kb on + strand, within ygjN at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.465 kb on - strand at 3232.471 kb on + strand at 3232.603 kb on + strand at 3232.603 kb on + strand at 3232.704 kb on - strand at 3232.704 kb on - strand at 3232.783 kb on + strand at 3232.783 kb on + strand at 3232.815 kb on + strand at 3232.830 kb on - strand at 3232.862 kb on + strand at 3232.862 kb on + strand at 3232.924 kb on - strand, within ygjO at 3232.967 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.979 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO at 3232.999 kb on - strand, within ygjO at 3233.263 kb on - strand, within ygjO at 3233.263 kb on - strand, within ygjO at 3233.289 kb on + strand, within ygjO at 3233.297 kb on - strand, within ygjO at 3233.297 kb on - strand, within ygjO at 3233.297 kb on - strand, within ygjO at 3233.352 kb on - strand, within ygjO at 3233.352 kb on - strand, within ygjO at 3233.358 kb on - strand, within ygjO at 3233.358 kb on - strand, within ygjO at 3233.402 kb on + strand, within ygjO at 3233.488 kb on + strand, within ygjO at 3233.488 kb on - strand, within ygjO at 3233.635 kb on - strand, within ygjO at 3233.671 kb on + strand, within ygjO at 3233.686 kb on + strand, within ygjO at 3233.692 kb on - strand, within ygjO at 3233.727 kb on - strand, within ygjO at 3233.727 kb on - strand, within ygjO at 3234.005 kb on + strand at 3234.008 kb on - strand at 3234.044 kb on + strand, within ygjP at 3234.111 kb on + strand, within ygjP at 3234.238 kb on + strand, within ygjP at 3234.297 kb on + strand, within ygjP at 3234.297 kb on + strand, within ygjP at 3234.297 kb on + strand, within ygjP at 3234.299 kb on - strand, within ygjP at 3234.341 kb on - strand, within ygjP at 3234.369 kb on + strand, within ygjP at 3234.411 kb on + strand, within ygjP at 3234.411 kb on + strand, within ygjP at 3234.413 kb on - strand, within ygjP at 3234.525 kb on + strand at 3234.544 kb on - strand at 3234.557 kb on + strand at 3234.708 kb on + strand, within ygjQ at 3234.716 kb on - strand, within ygjQ at 3234.793 kb on - strand, within ygjQ at 3234.805 kb on + strand, within ygjQ at 3234.805 kb on + strand, within ygjQ at 3234.813 kb on - strand, within ygjQ at 3234.876 kb on + strand, within ygjQ at 3234.892 kb on - strand, within ygjQ at 3234.892 kb on - strand, within ygjQ
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,231,812 - ygjM b3082 0.15 -1.3 3,231,855 - ygjM b3082 0.25 -0.6 3,231,856 - ygjM b3082 0.25 -0.6 3,231,892 - ygjM b3082 0.34 -0.9 3,231,892 - ygjM b3082 0.34 +2.7 3,232,179 + -1.5 3,232,179 + -0.0 3,232,181 + -0.6 3,232,186 + -1.8 3,232,189 - -0.3 3,232,233 + ygjN b3083 0.22 +1.3 3,232,267 + ygjN b3083 0.33 -0.1 3,232,272 - ygjN b3083 0.35 +0.4 3,232,302 + ygjN b3083 0.44 +0.5 3,232,394 + ygjN b3083 0.73 -1.2 3,232,416 + ygjN b3083 0.80 -0.4 3,232,465 - +1.7 3,232,465 - +0.4 3,232,465 - -0.2 3,232,471 + +1.6 3,232,603 + -0.9 3,232,603 + -0.2 3,232,704 - -0.2 3,232,704 - -1.4 3,232,783 + +3.5 3,232,783 + -1.6 3,232,815 + +1.6 3,232,830 - +0.1 3,232,862 + -0.2 3,232,862 + -1.1 3,232,924 - ygjO b3084 0.14 -0.2 3,232,967 - ygjO b3084 0.18 +2.2 3,232,979 - ygjO b3084 0.19 +0.9 3,232,979 - ygjO b3084 0.19 -0.6 3,232,999 - ygjO b3084 0.21 +1.4 3,232,999 - ygjO b3084 0.21 +0.8 3,233,263 - ygjO b3084 0.44 -2.4 3,233,263 - ygjO b3084 0.44 +1.2 3,233,289 + ygjO b3084 0.46 -0.4 3,233,297 - ygjO b3084 0.47 +0.4 3,233,297 - ygjO b3084 0.47 +0.4 3,233,297 - ygjO b3084 0.47 -0.6 3,233,352 - ygjO b3084 0.52 +0.4 3,233,352 - ygjO b3084 0.52 +0.7 3,233,358 - ygjO b3084 0.53 +1.4 3,233,358 - ygjO b3084 0.53 +1.7 3,233,402 + ygjO b3084 0.56 +2.7 3,233,488 + ygjO b3084 0.64 +2.1 3,233,488 - ygjO b3084 0.64 +1.5 3,233,635 - ygjO b3084 0.77 +1.0 3,233,671 + ygjO b3084 0.80 +0.5 3,233,686 + ygjO b3084 0.81 -1.4 3,233,692 - ygjO b3084 0.82 -0.9 3,233,727 - ygjO b3084 0.85 -1.5 3,233,727 - ygjO b3084 0.85 +3.5 3,234,005 + -0.9 3,234,008 - +2.3 3,234,044 + ygjP b3085 0.12 -0.9 3,234,111 + ygjP b3085 0.26 +2.0 3,234,238 + ygjP b3085 0.51 +1.0 3,234,297 + ygjP b3085 0.62 -0.9 3,234,297 + ygjP b3085 0.62 -0.6 3,234,297 + ygjP b3085 0.62 -0.3 3,234,299 - ygjP b3085 0.63 +0.4 3,234,341 - ygjP b3085 0.71 -1.4 3,234,369 + ygjP b3085 0.77 +2.3 3,234,411 + ygjP b3085 0.85 +0.2 3,234,411 + ygjP b3085 0.85 -2.9 3,234,413 - ygjP b3085 0.86 +0.4 3,234,525 + +1.5 3,234,544 - +1.4 3,234,557 + -0.0 3,234,708 + ygjQ b3086 0.21 -0.9 3,234,716 - ygjQ b3086 0.22 +0.8 3,234,793 - ygjQ b3086 0.33 -1.2 3,234,805 + ygjQ b3086 0.35 +3.7 3,234,805 + ygjQ b3086 0.35 -0.5 3,234,813 - ygjQ b3086 0.36 +0.4 3,234,876 + ygjQ b3086 0.45 +0.4 3,234,892 - ygjQ b3086 0.48 -2.1 3,234,892 - ygjQ b3086 0.48 +1.4
Or see this region's nucleotide sequence