Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolC and ygiB are separated by 147 nucleotides ygiB and ygiC are separated by 5 nucleotides
b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618
tolC
b3037: ygiB - conserved outer membrane protein (RefSeq), at 3,177,766 to 3,178,437
ygiB
b3038: ygiC - predicted enzyme (NCBI), at 3,178,443 to 3,179,603
ygiC
Position (kb)
3177
3178
3179 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3176.844 kb on - strand, within tolC at 3176.844 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.869 kb on - strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.887 kb on + strand, within tolC at 3176.932 kb on + strand, within tolC at 3176.934 kb on + strand, within tolC at 3176.942 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.979 kb on - strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.981 kb on + strand, within tolC at 3176.988 kb on - strand, within tolC at 3177.093 kb on + strand, within tolC at 3177.105 kb on + strand, within tolC at 3177.113 kb on - strand, within tolC at 3177.120 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.339 kb on + strand, within tolC at 3177.437 kb on + strand, within tolC at 3177.488 kb on + strand at 3177.516 kb on - strand at 3177.517 kb on + strand at 3177.517 kb on + strand at 3177.717 kb on + strand at 3177.737 kb on + strand at 3177.747 kb on - strand at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on + strand, within ygiB at 3177.964 kb on - strand, within ygiB at 3178.163 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.238 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.276 kb on + strand, within ygiB at 3178.294 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.298 kb on - strand, within ygiB at 3178.390 kb on + strand at 3178.390 kb on + strand at 3178.398 kb on - strand at 3178.423 kb on - strand at 3178.454 kb on + strand at 3178.540 kb on - strand at 3178.542 kb on + strand at 3178.545 kb on - strand at 3178.546 kb on - strand at 3178.546 kb on - strand at 3178.653 kb on - strand, within ygiC at 3178.754 kb on - strand, within ygiC at 3178.765 kb on - strand, within ygiC at 3178.900 kb on - strand, within ygiC at 3178.900 kb on - strand, within ygiC at 3178.900 kb on - strand, within ygiC at 3178.927 kb on + strand, within ygiC at 3178.927 kb on + strand, within ygiC at 3178.946 kb on + strand, within ygiC at 3178.989 kb on + strand, within ygiC at 3178.997 kb on - strand, within ygiC at 3179.016 kb on + strand, within ygiC at 3179.016 kb on + strand, within ygiC at 3179.016 kb on + strand, within ygiC at 3179.016 kb on + strand, within ygiC at 3179.024 kb on - strand, within ygiC at 3179.026 kb on + strand, within ygiC at 3179.057 kb on + strand, within ygiC at 3179.078 kb on - strand, within ygiC at 3179.078 kb on - strand, within ygiC at 3179.105 kb on + strand, within ygiC at 3179.179 kb on - strand, within ygiC at 3179.179 kb on - strand, within ygiC at 3179.196 kb on + strand, within ygiC at 3179.196 kb on + strand, within ygiC at 3179.253 kb on + strand, within ygiC
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,176,844 - tolC b3035 0.48 -0.2 3,176,844 - tolC b3035 0.48 +0.4 3,176,869 - tolC b3035 0.49 -0.9 3,176,869 - tolC b3035 0.49 +2.1 3,176,887 + tolC b3035 0.51 +1.1 3,176,887 + tolC b3035 0.51 +1.0 3,176,887 + tolC b3035 0.51 +0.1 3,176,932 + tolC b3035 0.54 +0.4 3,176,934 + tolC b3035 0.54 +1.5 3,176,942 - tolC b3035 0.54 -3.3 3,176,979 - tolC b3035 0.57 -0.9 3,176,979 - tolC b3035 0.57 +0.4 3,176,981 + tolC b3035 0.57 -1.2 3,176,981 + tolC b3035 0.57 -0.9 3,176,988 - tolC b3035 0.57 -1.2 3,177,093 + tolC b3035 0.65 -2.3 3,177,105 + tolC b3035 0.65 +0.9 3,177,113 - tolC b3035 0.66 -0.8 3,177,120 + tolC b3035 0.66 +1.4 3,177,339 + tolC b3035 0.81 +0.4 3,177,339 + tolC b3035 0.81 -0.2 3,177,437 + tolC b3035 0.88 -1.0 3,177,488 + -2.1 3,177,516 - +1.8 3,177,517 + -1.8 3,177,517 + +0.8 3,177,717 + +0.6 3,177,737 + -2.5 3,177,747 - +1.3 3,177,964 + ygiB b3037 0.29 -1.2 3,177,964 + ygiB b3037 0.29 -0.5 3,177,964 + ygiB b3037 0.29 +0.6 3,177,964 - ygiB b3037 0.29 -2.2 3,178,163 + ygiB b3037 0.59 -1.2 3,178,238 + ygiB b3037 0.70 -2.7 3,178,238 + ygiB b3037 0.70 -1.1 3,178,276 + ygiB b3037 0.76 +0.4 3,178,276 + ygiB b3037 0.76 -0.2 3,178,294 - ygiB b3037 0.79 -1.3 3,178,298 - ygiB b3037 0.79 -2.1 3,178,298 - ygiB b3037 0.79 -0.6 3,178,390 + -2.1 3,178,390 + -1.2 3,178,398 - -0.9 3,178,423 - -1.4 3,178,454 + -1.4 3,178,540 - -1.0 3,178,542 + -0.8 3,178,545 - -2.9 3,178,546 - -1.2 3,178,546 - -0.9 3,178,653 - ygiC b3038 0.18 -2.2 3,178,754 - ygiC b3038 0.27 -0.2 3,178,765 - ygiC b3038 0.28 -2.1 3,178,900 - ygiC b3038 0.39 -0.3 3,178,900 - ygiC b3038 0.39 -2.1 3,178,900 - ygiC b3038 0.39 +0.1 3,178,927 + ygiC b3038 0.42 -0.2 3,178,927 + ygiC b3038 0.42 -1.4 3,178,946 + ygiC b3038 0.43 +0.9 3,178,989 + ygiC b3038 0.47 -0.4 3,178,997 - ygiC b3038 0.48 -0.4 3,179,016 + ygiC b3038 0.49 -2.5 3,179,016 + ygiC b3038 0.49 +0.1 3,179,016 + ygiC b3038 0.49 -1.2 3,179,016 + ygiC b3038 0.49 +1.4 3,179,024 - ygiC b3038 0.50 +1.3 3,179,026 + ygiC b3038 0.50 -0.6 3,179,057 + ygiC b3038 0.53 -1.9 3,179,078 - ygiC b3038 0.55 -2.3 3,179,078 - ygiC b3038 0.55 +0.4 3,179,105 + ygiC b3038 0.57 -1.4 3,179,179 - ygiC b3038 0.63 -2.2 3,179,179 - ygiC b3038 0.63 -0.8 3,179,196 + ygiC b3038 0.65 -0.6 3,179,196 + ygiC b3038 0.65 +0.2 3,179,253 + ygiC b3038 0.70 +0.0
Or see this region's nucleotide sequence