Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metK and galP are separated by 423 nucleotides galP and sprT are separated by 76 nucleotides sprT and endA are separated by 94 nucleotides
b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882
metK
b2943: galP - galactose:H+ symporter (galP) (from data) , at 3,086,306 to 3,087,700
galP
b2944: sprT - hypothetical protein (NCBI), at 3,087,777 to 3,088,274
sprT
b2945: endA - DNA-specific endonuclease I (NCBI), at 3,088,369 to 3,089,076
endA
Position (kb)
3086
3087
3088 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.896 kb on + strand at 3085.984 kb on - strand at 3085.984 kb on - strand at 3086.143 kb on - strand at 3086.233 kb on - strand at 3086.330 kb on + strand at 3086.330 kb on + strand at 3086.370 kb on - strand at 3086.372 kb on + strand at 3086.373 kb on + strand at 3086.482 kb on - strand, within galP at 3086.482 kb on - strand, within galP at 3086.496 kb on + strand, within galP at 3086.660 kb on - strand, within galP at 3086.660 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.692 kb on - strand, within galP at 3086.703 kb on - strand, within galP at 3086.757 kb on + strand, within galP at 3086.780 kb on - strand, within galP at 3086.780 kb on - strand, within galP at 3086.797 kb on + strand, within galP at 3086.886 kb on + strand, within galP at 3086.886 kb on + strand, within galP at 3086.896 kb on - strand, within galP at 3086.924 kb on + strand, within galP at 3086.980 kb on + strand, within galP at 3087.004 kb on + strand, within galP at 3087.004 kb on + strand, within galP at 3087.028 kb on + strand, within galP at 3087.028 kb on + strand, within galP at 3087.061 kb on + strand, within galP at 3087.084 kb on - strand, within galP at 3087.102 kb on + strand, within galP at 3087.102 kb on + strand, within galP at 3087.156 kb on - strand, within galP at 3087.156 kb on - strand, within galP at 3087.183 kb on - strand, within galP at 3087.194 kb on + strand, within galP at 3087.194 kb on + strand, within galP at 3087.217 kb on + strand, within galP at 3087.241 kb on + strand, within galP at 3087.241 kb on + strand, within galP at 3087.261 kb on + strand, within galP at 3087.266 kb on + strand, within galP at 3087.266 kb on + strand, within galP at 3087.277 kb on + strand, within galP at 3087.307 kb on + strand, within galP at 3087.310 kb on - strand, within galP at 3087.315 kb on - strand, within galP at 3087.376 kb on - strand, within galP at 3087.414 kb on + strand, within galP at 3087.451 kb on - strand, within galP at 3087.451 kb on - strand, within galP at 3087.517 kb on + strand, within galP at 3087.517 kb on + strand, within galP at 3087.523 kb on - strand, within galP at 3087.525 kb on - strand, within galP at 3087.537 kb on - strand, within galP at 3087.572 kb on + strand at 3087.600 kb on + strand at 3087.704 kb on + strand at 3087.739 kb on - strand at 3087.759 kb on + strand at 3087.759 kb on + strand at 3087.802 kb on - strand at 3087.872 kb on - strand, within sprT at 3087.872 kb on - strand, within sprT at 3087.877 kb on - strand, within sprT at 3087.890 kb on + strand, within sprT at 3087.892 kb on + strand, within sprT at 3087.892 kb on + strand, within sprT at 3087.898 kb on - strand, within sprT at 3087.898 kb on - strand, within sprT at 3087.928 kb on + strand, within sprT at 3087.928 kb on + strand, within sprT at 3087.950 kb on + strand, within sprT at 3087.999 kb on + strand, within sprT at 3088.001 kb on - strand at 3088.001 kb on - strand, within sprT at 3088.048 kb on + strand, within sprT at 3088.048 kb on + strand, within sprT at 3088.099 kb on + strand, within sprT at 3088.350 kb on + strand at 3088.350 kb on + strand at 3088.406 kb on - strand at 3088.406 kb on - strand at 3088.453 kb on + strand, within endA at 3088.619 kb on - strand, within endA at 3088.683 kb on - strand, within endA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,085,886 + -1.2 3,085,886 + +0.4 3,085,886 + +0.1 3,085,896 + -0.2 3,085,984 - +1.9 3,085,984 - -1.8 3,086,143 - -0.8 3,086,233 - -2.3 3,086,330 + +2.9 3,086,330 + +1.4 3,086,370 - -0.6 3,086,372 + -1.4 3,086,373 + -1.5 3,086,482 - galP b2943 0.13 +0.1 3,086,482 - galP b2943 0.13 +1.2 3,086,496 + galP b2943 0.14 +0.5 3,086,660 - galP b2943 0.25 -0.3 3,086,660 - galP b2943 0.25 +1.7 3,086,681 - galP b2943 0.27 -0.5 3,086,681 - galP b2943 0.27 -1.4 3,086,692 - galP b2943 0.28 +0.7 3,086,703 - galP b2943 0.28 -1.2 3,086,757 + galP b2943 0.32 +0.9 3,086,780 - galP b2943 0.34 +1.6 3,086,780 - galP b2943 0.34 +0.5 3,086,797 + galP b2943 0.35 -1.4 3,086,886 + galP b2943 0.42 +1.6 3,086,886 + galP b2943 0.42 -1.6 3,086,896 - galP b2943 0.42 -0.8 3,086,924 + galP b2943 0.44 +2.1 3,086,980 + galP b2943 0.48 +0.1 3,087,004 + galP b2943 0.50 +2.5 3,087,004 + galP b2943 0.50 +2.1 3,087,028 + galP b2943 0.52 -0.9 3,087,028 + galP b2943 0.52 +3.7 3,087,061 + galP b2943 0.54 -0.9 3,087,084 - galP b2943 0.56 +0.4 3,087,102 + galP b2943 0.57 +0.3 3,087,102 + galP b2943 0.57 +0.6 3,087,156 - galP b2943 0.61 +0.5 3,087,156 - galP b2943 0.61 -1.7 3,087,183 - galP b2943 0.63 +1.4 3,087,194 + galP b2943 0.64 -0.9 3,087,194 + galP b2943 0.64 +1.0 3,087,217 + galP b2943 0.65 +0.2 3,087,241 + galP b2943 0.67 +0.7 3,087,241 + galP b2943 0.67 +1.5 3,087,261 + galP b2943 0.68 +0.4 3,087,266 + galP b2943 0.69 -2.8 3,087,266 + galP b2943 0.69 +1.8 3,087,277 + galP b2943 0.70 -2.3 3,087,307 + galP b2943 0.72 +0.1 3,087,310 - galP b2943 0.72 +1.1 3,087,315 - galP b2943 0.72 +1.4 3,087,376 - galP b2943 0.77 -0.0 3,087,414 + galP b2943 0.79 +1.7 3,087,451 - galP b2943 0.82 -1.2 3,087,451 - galP b2943 0.82 +3.1 3,087,517 + galP b2943 0.87 -2.5 3,087,517 + galP b2943 0.87 +1.4 3,087,523 - galP b2943 0.87 +1.4 3,087,525 - galP b2943 0.87 -0.6 3,087,537 - galP b2943 0.88 -0.6 3,087,572 + +0.2 3,087,600 + -1.1 3,087,704 + -1.2 3,087,739 - +2.2 3,087,759 + -1.4 3,087,759 + +2.7 3,087,802 - +2.8 3,087,872 - sprT b2944 0.19 -0.0 3,087,872 - sprT b2944 0.19 +0.1 3,087,877 - sprT b2944 0.20 +0.8 3,087,890 + sprT b2944 0.23 +1.7 3,087,892 + sprT b2944 0.23 -1.6 3,087,892 + sprT b2944 0.23 -0.0 3,087,898 - sprT b2944 0.24 +2.4 3,087,898 - sprT b2944 0.24 -2.3 3,087,928 + sprT b2944 0.30 +1.1 3,087,928 + sprT b2944 0.30 -1.2 3,087,950 + sprT b2944 0.35 +1.0 3,087,999 + sprT b2944 0.45 +1.6 3,088,001 - -0.2 3,088,001 - sprT b2944 0.45 -0.4 3,088,048 + sprT b2944 0.54 +0.8 3,088,048 + sprT b2944 0.54 +0.4 3,088,099 + sprT b2944 0.65 +1.4 3,088,350 + +1.9 3,088,350 + -1.2 3,088,406 - -1.2 3,088,406 - +1.0 3,088,453 + endA b2945 0.12 -0.9 3,088,619 - endA b2945 0.35 +0.4 3,088,683 - endA b2945 0.44 -0.7
Or see this region's nucleotide sequence