Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speA and yqgB are separated by 8 nucleotides yqgB and yqgC are separated by 135 nucleotides yqgC and metK are separated by 303 nucleotides metK and galP are separated by 423 nucleotides
b2938: speA - arginine decarboxylase (NCBI), at 3,081,957 to 3,083,933
speA
b2939: yqgB - predicted protein (RefSeq), at 3,083,942 to 3,084,073
yqgB
b2940: yqgC - hypothetical protein (NCBI), at 3,084,209 to 3,084,424
yqgC
b2942: metK - S-adenosylmethionine synthetase (NCBI), at 3,084,728 to 3,085,882
metK
b2943: galP - galactose:H+ symporter (galP) (from data) , at 3,086,306 to 3,087,700
galP
Position (kb)
3084
3085
3086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3083.761 kb on - strand at 3083.772 kb on - strand at 3083.802 kb on - strand at 3083.823 kb on + strand at 3083.834 kb on - strand at 3083.834 kb on - strand at 3083.872 kb on + strand at 3083.874 kb on + strand at 3083.880 kb on - strand at 3083.880 kb on - strand at 3083.911 kb on + strand at 3083.985 kb on + strand, within yqgB at 3084.042 kb on + strand, within yqgB at 3084.150 kb on - strand at 3084.153 kb on + strand at 3084.166 kb on - strand at 3084.181 kb on - strand at 3084.227 kb on + strand at 3084.242 kb on + strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.259 kb on - strand, within yqgC at 3084.285 kb on - strand, within yqgC at 3084.345 kb on - strand, within yqgC at 3084.471 kb on + strand at 3084.632 kb on + strand at 3084.632 kb on + strand at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.886 kb on + strand at 3085.896 kb on + strand at 3085.984 kb on - strand at 3085.984 kb on - strand at 3086.143 kb on - strand at 3086.233 kb on - strand at 3086.330 kb on + strand at 3086.330 kb on + strand at 3086.370 kb on - strand at 3086.372 kb on + strand at 3086.373 kb on + strand at 3086.482 kb on - strand, within galP at 3086.482 kb on - strand, within galP at 3086.496 kb on + strand, within galP at 3086.660 kb on - strand, within galP at 3086.660 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.681 kb on - strand, within galP at 3086.692 kb on - strand, within galP at 3086.703 kb on - strand, within galP at 3086.757 kb on + strand, within galP at 3086.780 kb on - strand, within galP at 3086.780 kb on - strand, within galP at 3086.797 kb on + strand, within galP
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,083,761 - -1.2 3,083,772 - -0.9 3,083,802 - -0.9 3,083,823 + -0.8 3,083,834 - -1.9 3,083,834 - -0.2 3,083,872 + -2.2 3,083,874 + -0.8 3,083,880 - -1.9 3,083,880 - -0.9 3,083,911 + -0.4 3,083,985 + yqgB b2939 0.33 -0.2 3,084,042 + yqgB b2939 0.76 +0.8 3,084,150 - +0.4 3,084,153 + +0.3 3,084,166 - -0.2 3,084,181 - +1.8 3,084,227 + +0.3 3,084,242 + yqgC b2940 0.15 -3.6 3,084,259 - yqgC b2940 0.23 +0.4 3,084,259 - yqgC b2940 0.23 -1.4 3,084,259 - yqgC b2940 0.23 +0.4 3,084,285 - yqgC b2940 0.35 +1.2 3,084,345 - yqgC b2940 0.63 +1.0 3,084,471 + +1.6 3,084,632 + +1.2 3,084,632 + -0.2 3,085,886 + -1.2 3,085,886 + +0.4 3,085,886 + +0.1 3,085,896 + -0.2 3,085,984 - +1.9 3,085,984 - -1.8 3,086,143 - -0.8 3,086,233 - -2.3 3,086,330 + +2.9 3,086,330 + +1.4 3,086,370 - -0.6 3,086,372 + -1.4 3,086,373 + -1.5 3,086,482 - galP b2943 0.13 +0.1 3,086,482 - galP b2943 0.13 +1.2 3,086,496 + galP b2943 0.14 +0.5 3,086,660 - galP b2943 0.25 -0.3 3,086,660 - galP b2943 0.25 +1.7 3,086,681 - galP b2943 0.27 -0.5 3,086,681 - galP b2943 0.27 -1.4 3,086,692 - galP b2943 0.28 +0.7 3,086,703 - galP b2943 0.28 -1.2 3,086,757 + galP b2943 0.32 +0.9 3,086,780 - galP b2943 0.34 +1.6 3,086,780 - galP b2943 0.34 +0.5 3,086,797 + galP b2943 0.35 -1.4
Or see this region's nucleotide sequence