Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argK and ygfG are separated by 10 nucleotides ygfG and ygfH are separated by 23 nucleotides ygfH and ygfI overlap by 4 nucleotides
b2918: argK - arginine/ornithine transport system ATPase (NCBI), at 3,061,009 to 3,062,004
argK
b2919: ygfG - putative enzyme (VIMSS), at 3,062,015 to 3,062,800
ygfG
b2920: ygfH - propionyl-CoA:succinate-CoA transferase (NCBI), at 3,062,824 to 3,064,302
ygfH
b2921: ygfI - putative transcriptional regulator LYSR-type (VIMSS), at 3,064,299 to 3,065,195
ygfI
Position (kb)
3062
3063
3064
3065 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3062.118 kb on + strand, within ygfG at 3062.216 kb on + strand, within ygfG at 3062.251 kb on + strand, within ygfG at 3062.256 kb on + strand, within ygfG at 3062.256 kb on + strand, within ygfG at 3062.328 kb on + strand, within ygfG at 3062.328 kb on + strand, within ygfG at 3062.364 kb on + strand, within ygfG at 3062.390 kb on + strand, within ygfG at 3062.411 kb on + strand, within ygfG at 3062.438 kb on - strand, within ygfG at 3062.438 kb on - strand, within ygfG at 3062.608 kb on - strand, within ygfG at 3062.608 kb on - strand, within ygfG at 3062.651 kb on + strand, within ygfG at 3062.723 kb on + strand at 3062.731 kb on - strand at 3062.731 kb on - strand at 3062.785 kb on - strand at 3062.929 kb on + strand at 3062.937 kb on - strand at 3062.937 kb on - strand at 3062.937 kb on - strand at 3062.963 kb on - strand at 3063.104 kb on + strand, within ygfH at 3063.314 kb on - strand, within ygfH at 3063.314 kb on - strand, within ygfH at 3063.372 kb on + strand, within ygfH at 3063.422 kb on - strand, within ygfH at 3063.432 kb on - strand, within ygfH at 3063.432 kb on - strand, within ygfH at 3063.695 kb on - strand, within ygfH at 3063.851 kb on - strand, within ygfH at 3064.041 kb on - strand, within ygfH at 3064.102 kb on + strand, within ygfH at 3064.102 kb on + strand, within ygfH at 3064.127 kb on + strand, within ygfH at 3064.127 kb on + strand, within ygfH at 3064.145 kb on - strand, within ygfH at 3064.154 kb on - strand, within ygfH at 3064.174 kb on + strand at 3064.174 kb on + strand at 3064.174 kb on - strand at 3064.176 kb on + strand at 3064.187 kb on + strand at 3064.187 kb on + strand at 3064.188 kb on + strand at 3064.222 kb on + strand at 3064.240 kb on + strand at 3064.241 kb on + strand at 3064.241 kb on + strand at 3064.249 kb on - strand at 3064.291 kb on + strand at 3064.298 kb on + strand at 3064.298 kb on + strand at 3064.298 kb on + strand at 3064.298 kb on + strand at 3064.298 kb on + strand at 3064.299 kb on - strand at 3064.306 kb on - strand at 3064.306 kb on - strand at 3064.306 kb on - strand at 3064.306 kb on - strand at 3064.330 kb on + strand at 3064.330 kb on - strand at 3064.332 kb on + strand at 3064.389 kb on + strand, within ygfI at 3064.391 kb on - strand, within ygfI at 3064.395 kb on - strand, within ygfI at 3064.397 kb on - strand, within ygfI at 3064.397 kb on - strand, within ygfI at 3064.397 kb on - strand, within ygfI at 3064.399 kb on + strand, within ygfI at 3064.402 kb on - strand, within ygfI at 3064.404 kb on + strand, within ygfI at 3064.407 kb on - strand, within ygfI at 3064.407 kb on - strand, within ygfI at 3064.407 kb on - strand, within ygfI at 3064.419 kb on - strand, within ygfI at 3064.435 kb on + strand, within ygfI at 3064.438 kb on - strand, within ygfI at 3064.466 kb on - strand, within ygfI at 3064.467 kb on + strand, within ygfI at 3064.490 kb on + strand, within ygfI at 3064.493 kb on - strand, within ygfI at 3064.498 kb on - strand, within ygfI at 3064.534 kb on - strand, within ygfI at 3064.535 kb on + strand, within ygfI at 3064.555 kb on - strand, within ygfI at 3064.555 kb on - strand, within ygfI at 3064.555 kb on - strand, within ygfI at 3064.585 kb on + strand, within ygfI at 3064.585 kb on + strand, within ygfI at 3064.615 kb on + strand, within ygfI at 3064.627 kb on + strand, within ygfI at 3064.636 kb on - strand, within ygfI at 3064.638 kb on + strand, within ygfI at 3064.663 kb on - strand, within ygfI at 3064.663 kb on - strand, within ygfI at 3064.702 kb on - strand, within ygfI at 3064.708 kb on - strand, within ygfI at 3064.708 kb on - strand, within ygfI at 3064.743 kb on - strand, within ygfI at 3064.817 kb on + strand, within ygfI at 3064.817 kb on + strand, within ygfI at 3064.837 kb on + strand, within ygfI at 3064.837 kb on + strand, within ygfI at 3064.837 kb on + strand, within ygfI at 3064.837 kb on + strand, within ygfI at 3064.837 kb on + strand, within ygfI at 3064.845 kb on - strand, within ygfI at 3064.848 kb on + strand, within ygfI at 3064.848 kb on + strand, within ygfI at 3064.858 kb on + strand, within ygfI at 3064.858 kb on + strand, within ygfI at 3064.891 kb on + strand, within ygfI at 3064.891 kb on + strand, within ygfI at 3064.975 kb on + strand, within ygfI at 3064.997 kb on + strand, within ygfI at 3064.997 kb on + strand, within ygfI at 3065.056 kb on - strand, within ygfI at 3065.072 kb on + strand, within ygfI at 3065.125 kb on - strand at 3065.226 kb on - strand at 3065.244 kb on + strand at 3065.245 kb on - strand at 3065.259 kb on + strand at 3065.270 kb on - strand at 3065.284 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 3,062,118 + ygfG b2919 0.13 +1.9 3,062,216 + ygfG b2919 0.26 -0.0 3,062,251 + ygfG b2919 0.30 +0.1 3,062,256 + ygfG b2919 0.31 +0.7 3,062,256 + ygfG b2919 0.31 -1.2 3,062,328 + ygfG b2919 0.40 -1.4 3,062,328 + ygfG b2919 0.40 +1.4 3,062,364 + ygfG b2919 0.44 -0.2 3,062,390 + ygfG b2919 0.48 -1.2 3,062,411 + ygfG b2919 0.50 -0.4 3,062,438 - ygfG b2919 0.54 +0.4 3,062,438 - ygfG b2919 0.54 +0.9 3,062,608 - ygfG b2919 0.75 +1.7 3,062,608 - ygfG b2919 0.75 -1.8 3,062,651 + ygfG b2919 0.81 +0.8 3,062,723 + -0.6 3,062,731 - -1.4 3,062,731 - -0.4 3,062,785 - -1.6 3,062,929 + +1.6 3,062,937 - +1.3 3,062,937 - -0.5 3,062,937 - -2.3 3,062,963 - -0.7 3,063,104 + ygfH b2920 0.19 -1.1 3,063,314 - ygfH b2920 0.33 +0.8 3,063,314 - ygfH b2920 0.33 +1.2 3,063,372 + ygfH b2920 0.37 +0.9 3,063,422 - ygfH b2920 0.40 +0.4 3,063,432 - ygfH b2920 0.41 +0.4 3,063,432 - ygfH b2920 0.41 +0.8 3,063,695 - ygfH b2920 0.59 +0.2 3,063,851 - ygfH b2920 0.69 -1.1 3,064,041 - ygfH b2920 0.82 -0.1 3,064,102 + ygfH b2920 0.86 -0.9 3,064,102 + ygfH b2920 0.86 +1.4 3,064,127 + ygfH b2920 0.88 -0.8 3,064,127 + ygfH b2920 0.88 -0.8 3,064,145 - ygfH b2920 0.89 -0.1 3,064,154 - ygfH b2920 0.90 -1.6 3,064,174 + -1.8 3,064,174 + -0.0 3,064,174 - +1.0 3,064,176 + -0.4 3,064,187 + -1.6 3,064,187 + -3.1 3,064,188 + +0.1 3,064,222 + +2.9 3,064,240 + -1.2 3,064,241 + +0.2 3,064,241 + +1.4 3,064,249 - -0.7 3,064,291 + -0.6 3,064,298 + -2.7 3,064,298 + +0.7 3,064,298 + -0.4 3,064,298 + +1.4 3,064,298 + -0.8 3,064,299 - -2.7 3,064,306 - -1.8 3,064,306 - +0.2 3,064,306 - +0.7 3,064,306 - +1.5 3,064,330 + -1.9 3,064,330 - +0.0 3,064,332 + +0.3 3,064,389 + ygfI b2921 0.10 +0.1 3,064,391 - ygfI b2921 0.10 +0.6 3,064,395 - ygfI b2921 0.11 -0.5 3,064,397 - ygfI b2921 0.11 +1.2 3,064,397 - ygfI b2921 0.11 -1.3 3,064,397 - ygfI b2921 0.11 +1.2 3,064,399 + ygfI b2921 0.11 +1.2 3,064,402 - ygfI b2921 0.11 +0.4 3,064,404 + ygfI b2921 0.12 -0.1 3,064,407 - ygfI b2921 0.12 -1.6 3,064,407 - ygfI b2921 0.12 -0.9 3,064,407 - ygfI b2921 0.12 -0.9 3,064,419 - ygfI b2921 0.13 +0.8 3,064,435 + ygfI b2921 0.15 +1.2 3,064,438 - ygfI b2921 0.15 -1.6 3,064,466 - ygfI b2921 0.19 +2.6 3,064,467 + ygfI b2921 0.19 -0.5 3,064,490 + ygfI b2921 0.21 -0.9 3,064,493 - ygfI b2921 0.22 +2.9 3,064,498 - ygfI b2921 0.22 -0.6 3,064,534 - ygfI b2921 0.26 -1.3 3,064,535 + ygfI b2921 0.26 -1.6 3,064,555 - ygfI b2921 0.29 -0.3 3,064,555 - ygfI b2921 0.29 +0.8 3,064,555 - ygfI b2921 0.29 +1.4 3,064,585 + ygfI b2921 0.32 -1.5 3,064,585 + ygfI b2921 0.32 -1.3 3,064,615 + ygfI b2921 0.35 -0.5 3,064,627 + ygfI b2921 0.37 +0.8 3,064,636 - ygfI b2921 0.38 -0.6 3,064,638 + ygfI b2921 0.38 +0.4 3,064,663 - ygfI b2921 0.41 +1.7 3,064,663 - ygfI b2921 0.41 -0.5 3,064,702 - ygfI b2921 0.45 -1.2 3,064,708 - ygfI b2921 0.46 +0.8 3,064,708 - ygfI b2921 0.46 +0.2 3,064,743 - ygfI b2921 0.49 +1.1 3,064,817 + ygfI b2921 0.58 -1.9 3,064,817 + ygfI b2921 0.58 +0.4 3,064,837 + ygfI b2921 0.60 +0.5 3,064,837 + ygfI b2921 0.60 +0.8 3,064,837 + ygfI b2921 0.60 -0.1 3,064,837 + ygfI b2921 0.60 -2.3 3,064,837 + ygfI b2921 0.60 +0.4 3,064,845 - ygfI b2921 0.61 +0.6 3,064,848 + ygfI b2921 0.61 +0.4 3,064,848 + ygfI b2921 0.61 +2.1 3,064,858 + ygfI b2921 0.62 +1.2 3,064,858 + ygfI b2921 0.62 -1.9 3,064,891 + ygfI b2921 0.66 +0.2 3,064,891 + ygfI b2921 0.66 -0.2 3,064,975 + ygfI b2921 0.75 -3.4 3,064,997 + ygfI b2921 0.78 -0.6 3,064,997 + ygfI b2921 0.78 -0.2 3,065,056 - ygfI b2921 0.84 -2.9 3,065,072 + ygfI b2921 0.86 -0.0 3,065,125 - -0.2 3,065,226 - -0.5 3,065,244 + -1.4 3,065,245 - +1.4 3,065,259 + +0.6 3,065,270 - -0.8 3,065,284 + +0.3
Or see this region's nucleotide sequence