Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbK and ygbL are separated by 91 nucleotides ygbL and ygbM are separated by 4 nucleotides ygbM and ygbN are separated by 88 nucleotides
b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523
ygbK
b2738: ygbL - hypothetical protein (NCBI), at 2,861,615 to 2,862,253
ygbL
b2739: ygbM - hypothetical protein (NCBI), at 2,862,258 to 2,863,034
ygbM
b2740: ygbN - predicted transporter (NCBI), at 2,863,123 to 2,864,487
ygbN
Position (kb)
2861
2862
2863 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2860.657 kb on + strand, within ygbK at 2860.721 kb on + strand, within ygbK at 2860.847 kb on - strand, within ygbK at 2860.851 kb on + strand, within ygbK at 2860.895 kb on - strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.997 kb on + strand, within ygbK at 2860.998 kb on - strand, within ygbK at 2861.029 kb on - strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.080 kb on + strand, within ygbK at 2861.111 kb on + strand, within ygbK at 2861.166 kb on - strand, within ygbK at 2861.195 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.518 kb on + strand at 2861.531 kb on + strand at 2861.531 kb on + strand at 2861.539 kb on - strand at 2861.539 kb on - strand at 2861.551 kb on + strand at 2861.602 kb on - strand at 2861.614 kb on - strand at 2861.614 kb on - strand at 2861.719 kb on - strand, within ygbL at 2861.766 kb on - strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.778 kb on + strand, within ygbL at 2861.832 kb on + strand, within ygbL at 2861.924 kb on - strand, within ygbL at 2861.984 kb on - strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.001 kb on + strand, within ygbL at 2862.384 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.439 kb on - strand, within ygbM at 2862.479 kb on - strand, within ygbM at 2862.511 kb on - strand, within ygbM at 2862.531 kb on + strand, within ygbM at 2862.569 kb on - strand, within ygbM at 2862.569 kb on - strand, within ygbM at 2862.598 kb on + strand, within ygbM at 2862.608 kb on + strand, within ygbM at 2862.660 kb on - strand, within ygbM at 2862.695 kb on - strand, within ygbM at 2862.708 kb on - strand, within ygbM at 2862.741 kb on - strand, within ygbM at 2862.743 kb on + strand, within ygbM at 2862.743 kb on + strand, within ygbM at 2862.753 kb on - strand, within ygbM at 2862.756 kb on - strand, within ygbM at 2862.767 kb on + strand, within ygbM at 2862.775 kb on - strand, within ygbM at 2862.775 kb on - strand, within ygbM at 2862.898 kb on - strand, within ygbM at 2862.903 kb on - strand, within ygbM at 2862.903 kb on - strand, within ygbM at 2862.936 kb on - strand, within ygbM at 2862.947 kb on + strand, within ygbM at 2862.947 kb on + strand, within ygbM at 2862.967 kb on - strand at 2862.997 kb on + strand at 2863.069 kb on - strand at 2863.084 kb on - strand at 2863.114 kb on + strand at 2863.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,860,657 + ygbK b2737 0.26 -0.7 2,860,721 + ygbK b2737 0.31 -1.3 2,860,847 - ygbK b2737 0.42 -0.7 2,860,851 + ygbK b2737 0.42 +0.9 2,860,895 - ygbK b2737 0.46 +1.6 2,860,990 + ygbK b2737 0.54 -0.7 2,860,990 + ygbK b2737 0.54 +2.2 2,860,997 + ygbK b2737 0.55 -0.3 2,860,998 - ygbK b2737 0.55 +0.5 2,861,029 - ygbK b2737 0.58 -1.2 2,861,044 + ygbK b2737 0.59 -1.0 2,861,044 + ygbK b2737 0.59 +1.7 2,861,080 + ygbK b2737 0.62 -0.4 2,861,111 + ygbK b2737 0.65 -0.3 2,861,166 - ygbK b2737 0.69 +2.6 2,861,195 + ygbK b2737 0.72 -2.2 2,861,301 + ygbK b2737 0.81 +0.3 2,861,301 + ygbK b2737 0.81 -0.7 2,861,518 + -0.1 2,861,531 + +0.3 2,861,531 + +0.1 2,861,539 - -1.2 2,861,539 - -0.4 2,861,551 + -0.5 2,861,602 - -0.7 2,861,614 - -0.3 2,861,614 - +1.9 2,861,719 - ygbL b2738 0.16 +0.6 2,861,766 - ygbL b2738 0.24 -0.9 2,861,778 + ygbL b2738 0.26 +1.4 2,861,778 + ygbL b2738 0.26 -2.4 2,861,832 + ygbL b2738 0.34 +0.1 2,861,924 - ygbL b2738 0.48 +2.8 2,861,984 - ygbL b2738 0.58 +3.1 2,862,001 + ygbL b2738 0.60 -1.0 2,862,001 + ygbL b2738 0.60 -0.9 2,862,384 - ygbM b2739 0.16 +0.8 2,862,439 - ygbM b2739 0.23 -0.1 2,862,439 - ygbM b2739 0.23 +0.2 2,862,479 - ygbM b2739 0.28 -0.0 2,862,511 - ygbM b2739 0.33 -1.0 2,862,531 + ygbM b2739 0.35 +0.6 2,862,569 - ygbM b2739 0.40 -0.5 2,862,569 - ygbM b2739 0.40 +0.3 2,862,598 + ygbM b2739 0.44 -0.7 2,862,608 + ygbM b2739 0.45 -0.7 2,862,660 - ygbM b2739 0.52 +0.3 2,862,695 - ygbM b2739 0.56 -0.0 2,862,708 - ygbM b2739 0.58 +0.0 2,862,741 - ygbM b2739 0.62 -0.7 2,862,743 + ygbM b2739 0.62 -2.0 2,862,743 + ygbM b2739 0.62 -0.3 2,862,753 - ygbM b2739 0.64 +0.2 2,862,756 - ygbM b2739 0.64 +1.9 2,862,767 + ygbM b2739 0.66 +1.1 2,862,775 - ygbM b2739 0.67 +2.3 2,862,775 - ygbM b2739 0.67 -1.0 2,862,898 - ygbM b2739 0.82 -0.3 2,862,903 - ygbM b2739 0.83 -0.7 2,862,903 - ygbM b2739 0.83 -1.2 2,862,936 - ygbM b2739 0.87 -1.4 2,862,947 + ygbM b2739 0.89 +0.7 2,862,947 + ygbM b2739 0.89 -1.3 2,862,967 - +1.1 2,862,997 + +0.8 2,863,069 - +0.4 2,863,084 - -2.3 2,863,114 + -0.4 2,863,140 + +0.5
Or see this region's nucleotide sequence