Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygbI and ygbJ are separated by 195 nucleotides ygbJ and ygbK overlap by 4 nucleotides
b2735: ygbI - predicted DNA-binding transcriptional regulator (RefSeq), at 2,858,489 to 2,859,256
ygbI
b2736: ygbJ - predicted dehydrogenase, with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,859,452 to 2,860,360
ygbJ
b2737: ygbK - hypothetical protein (NCBI), at 2,860,357 to 2,861,523
ygbK
Position (kb)
2859
2860
2861 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2858.457 kb on + strand at 2858.457 kb on - strand at 2858.466 kb on - strand at 2858.471 kb on - strand at 2858.471 kb on - strand at 2858.473 kb on - strand at 2858.476 kb on - strand at 2858.514 kb on + strand at 2858.518 kb on + strand at 2858.568 kb on + strand, within ygbI at 2858.568 kb on + strand, within ygbI at 2858.618 kb on - strand, within ygbI at 2858.719 kb on + strand, within ygbI at 2858.822 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.827 kb on - strand, within ygbI at 2858.912 kb on - strand, within ygbI at 2859.114 kb on - strand, within ygbI at 2859.160 kb on - strand, within ygbI at 2859.528 kb on - strand at 2859.528 kb on - strand at 2859.539 kb on - strand at 2859.664 kb on + strand, within ygbJ at 2859.852 kb on - strand, within ygbJ at 2859.962 kb on - strand, within ygbJ at 2859.965 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.243 kb on + strand, within ygbJ at 2860.296 kb on + strand at 2860.464 kb on - strand at 2860.540 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.598 kb on - strand, within ygbK at 2860.657 kb on + strand, within ygbK at 2860.721 kb on + strand, within ygbK at 2860.847 kb on - strand, within ygbK at 2860.851 kb on + strand, within ygbK at 2860.895 kb on - strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.990 kb on + strand, within ygbK at 2860.997 kb on + strand, within ygbK at 2860.998 kb on - strand, within ygbK at 2861.029 kb on - strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.044 kb on + strand, within ygbK at 2861.080 kb on + strand, within ygbK at 2861.111 kb on + strand, within ygbK at 2861.166 kb on - strand, within ygbK at 2861.195 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK at 2861.301 kb on + strand, within ygbK
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,858,457 + -0.7 2,858,457 - -0.3 2,858,466 - -1.5 2,858,471 - +1.3 2,858,471 - -0.7 2,858,473 - -1.5 2,858,476 - +0.2 2,858,514 + +0.9 2,858,518 + +0.1 2,858,568 + ygbI b2735 0.10 +1.6 2,858,568 + ygbI b2735 0.10 +1.5 2,858,618 - ygbI b2735 0.17 -1.7 2,858,719 + ygbI b2735 0.30 +0.5 2,858,822 - ygbI b2735 0.43 -0.8 2,858,827 - ygbI b2735 0.44 -1.0 2,858,827 - ygbI b2735 0.44 -2.0 2,858,912 - ygbI b2735 0.55 +1.1 2,859,114 - ygbI b2735 0.81 -0.0 2,859,160 - ygbI b2735 0.87 -2.5 2,859,528 - +0.1 2,859,528 - +0.3 2,859,539 - +0.3 2,859,664 + ygbJ b2736 0.23 +1.1 2,859,852 - ygbJ b2736 0.44 +1.5 2,859,962 - ygbJ b2736 0.56 +0.9 2,859,965 + ygbJ b2736 0.56 +0.7 2,860,243 + ygbJ b2736 0.87 +0.6 2,860,243 + ygbJ b2736 0.87 +2.6 2,860,296 + +1.2 2,860,464 - +0.7 2,860,540 - ygbK b2737 0.16 +0.6 2,860,598 - ygbK b2737 0.21 -0.7 2,860,598 - ygbK b2737 0.21 -1.3 2,860,657 + ygbK b2737 0.26 -0.7 2,860,721 + ygbK b2737 0.31 -1.3 2,860,847 - ygbK b2737 0.42 -0.7 2,860,851 + ygbK b2737 0.42 +0.9 2,860,895 - ygbK b2737 0.46 +1.6 2,860,990 + ygbK b2737 0.54 -0.7 2,860,990 + ygbK b2737 0.54 +2.2 2,860,997 + ygbK b2737 0.55 -0.3 2,860,998 - ygbK b2737 0.55 +0.5 2,861,029 - ygbK b2737 0.58 -1.2 2,861,044 + ygbK b2737 0.59 -1.0 2,861,044 + ygbK b2737 0.59 +1.7 2,861,080 + ygbK b2737 0.62 -0.4 2,861,111 + ygbK b2737 0.65 -0.3 2,861,166 - ygbK b2737 0.69 +2.6 2,861,195 + ygbK b2737 0.72 -2.2 2,861,301 + ygbK b2737 0.81 +0.3 2,861,301 + ygbK b2737 0.81 -0.7
Or see this region's nucleotide sequence