Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycC and hycB overlap by 4 nucleotides hycB and hycA are separated by 124 nucleotides hycA and hypA are separated by 211 nucleotides
b2723: hycC - NADH dehydrogenase subunit N (NCBI), at 2,845,437 to 2,847,263
hycC
b2724: hycB - hydrogenase 3, Fe-S subunit (NCBI), at 2,847,260 to 2,847,871
hycB
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019
hypA
Position (kb)
2847
2848 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2846.264 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.269 kb on + strand, within hycC at 2846.272 kb on - strand, within hycC at 2846.345 kb on - strand, within hycC at 2846.381 kb on + strand, within hycC at 2846.406 kb on - strand, within hycC at 2846.406 kb on - strand, within hycC at 2846.475 kb on - strand, within hycC at 2846.506 kb on - strand, within hycC at 2846.699 kb on + strand, within hycC at 2846.699 kb on + strand, within hycC at 2846.731 kb on - strand, within hycC at 2846.810 kb on - strand, within hycC at 2846.886 kb on - strand, within hycC at 2846.886 kb on - strand, within hycC at 2846.941 kb on - strand, within hycC at 2846.953 kb on + strand, within hycC at 2846.953 kb on + strand, within hycC at 2846.977 kb on + strand, within hycC at 2846.985 kb on - strand, within hycC at 2846.985 kb on - strand, within hycC at 2847.072 kb on - strand, within hycC at 2847.099 kb on + strand at 2847.139 kb on - strand at 2847.276 kb on - strand at 2847.353 kb on + strand, within hycB at 2847.363 kb on + strand, within hycB at 2847.421 kb on + strand, within hycB at 2847.437 kb on - strand, within hycB at 2847.455 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on + strand, within hycB at 2847.621 kb on - strand, within hycB at 2847.644 kb on + strand, within hycB at 2847.703 kb on + strand, within hycB at 2847.711 kb on - strand, within hycB at 2847.722 kb on - strand, within hycB at 2847.761 kb on - strand, within hycB at 2847.822 kb on - strand at 2847.829 kb on + strand at 2847.829 kb on + strand at 2847.888 kb on - strand at 2847.953 kb on - strand at 2848.071 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.082 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.719 kb on - strand, within hypA at 2848.850 kb on - strand, within hypA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,846,264 + hycC b2723 0.45 +0.8 2,846,269 + hycC b2723 0.46 -1.0 2,846,269 + hycC b2723 0.46 +1.9 2,846,269 + hycC b2723 0.46 +1.3 2,846,269 + hycC b2723 0.46 -0.8 2,846,272 - hycC b2723 0.46 -1.7 2,846,345 - hycC b2723 0.50 -0.8 2,846,381 + hycC b2723 0.52 -1.1 2,846,406 - hycC b2723 0.53 +2.0 2,846,406 - hycC b2723 0.53 -0.7 2,846,475 - hycC b2723 0.57 +0.2 2,846,506 - hycC b2723 0.59 -0.2 2,846,699 + hycC b2723 0.69 +2.3 2,846,699 + hycC b2723 0.69 +0.3 2,846,731 - hycC b2723 0.71 -0.2 2,846,810 - hycC b2723 0.75 -0.1 2,846,886 - hycC b2723 0.79 -1.8 2,846,886 - hycC b2723 0.79 -1.0 2,846,941 - hycC b2723 0.82 +0.3 2,846,953 + hycC b2723 0.83 -2.1 2,846,953 + hycC b2723 0.83 +0.3 2,846,977 + hycC b2723 0.84 -1.9 2,846,985 - hycC b2723 0.85 -0.7 2,846,985 - hycC b2723 0.85 -1.2 2,847,072 - hycC b2723 0.89 +1.3 2,847,099 + -1.1 2,847,139 - -1.2 2,847,276 - -0.2 2,847,353 + hycB b2724 0.15 +2.2 2,847,363 + hycB b2724 0.17 -2.1 2,847,421 + hycB b2724 0.26 +2.7 2,847,437 - hycB b2724 0.29 +1.3 2,847,455 + hycB b2724 0.32 -0.8 2,847,621 + hycB b2724 0.59 -0.8 2,847,621 + hycB b2724 0.59 +0.8 2,847,621 - hycB b2724 0.59 +4.0 2,847,644 + hycB b2724 0.63 -1.5 2,847,703 + hycB b2724 0.72 -0.7 2,847,711 - hycB b2724 0.74 +2.3 2,847,722 - hycB b2724 0.75 +1.4 2,847,761 - hycB b2724 0.82 +0.9 2,847,822 - +1.6 2,847,829 + -0.2 2,847,829 + +0.5 2,847,888 - +0.4 2,847,953 - +1.3 2,848,071 - hycA b2725 0.16 +0.9 2,848,073 - hycA b2725 0.17 -0.7 2,848,073 - hycA b2725 0.17 -1.9 2,848,082 - hycA b2725 0.19 -0.4 2,848,153 - hycA b2725 0.34 +0.3 2,848,153 - hycA b2725 0.34 +0.3 2,848,157 + hycA b2725 0.35 +1.3 2,848,157 + hycA b2725 0.35 -1.2 2,848,253 - hycA b2725 0.56 -1.7 2,848,253 - hycA b2725 0.56 +1.0 2,848,684 - -0.1 2,848,684 - +1.3 2,848,684 - -1.0 2,848,719 - hypA b2726 0.14 -0.2 2,848,850 - hypA b2726 0.52 +1.3
Or see this region's nucleotide sequence