Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt norV and norW overlap by 4 nucleotides norW and hypF are separated by 127 nucleotides
b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937
norV
b2711: norW - nitric oxide reductase (NCBI), at 2,831,934 to 2,833,067
norW
b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447
hypF
Position (kb)
2831
2832
2833
2834 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2830.983 kb on + strand, within norV at 2830.986 kb on - strand, within norV at 2830.986 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.092 kb on - strand, within norV at 2831.109 kb on + strand, within norV at 2831.134 kb on + strand, within norV at 2831.273 kb on + strand, within norV at 2831.273 kb on + strand, within norV at 2831.314 kb on + strand, within norV at 2831.314 kb on + strand, within norV at 2831.629 kb on + strand, within norV at 2831.646 kb on - strand, within norV at 2831.646 kb on - strand, within norV at 2831.651 kb on + strand, within norV at 2831.706 kb on - strand, within norV at 2831.732 kb on - strand, within norV at 2831.748 kb on + strand, within norV at 2831.748 kb on + strand, within norV at 2831.829 kb on + strand at 2831.934 kb on - strand at 2831.957 kb on - strand at 2832.048 kb on + strand, within norW at 2832.064 kb on - strand, within norW at 2832.150 kb on - strand, within norW at 2832.281 kb on + strand, within norW at 2832.281 kb on + strand, within norW at 2832.364 kb on + strand, within norW at 2832.384 kb on - strand, within norW at 2832.384 kb on - strand, within norW at 2832.526 kb on + strand, within norW at 2832.896 kb on - strand, within norW at 2832.956 kb on + strand at 2833.005 kb on + strand at 2833.005 kb on + strand at 2833.046 kb on + strand at 2833.054 kb on - strand at 2833.119 kb on + strand at 2833.119 kb on + strand at 2833.350 kb on + strand at 2833.350 kb on + strand at 2833.353 kb on - strand at 2833.371 kb on + strand at 2833.399 kb on - strand at 2833.399 kb on - strand at 2833.408 kb on + strand at 2833.423 kb on + strand, within hypF at 2833.423 kb on + strand, within hypF at 2833.581 kb on + strand, within hypF at 2833.699 kb on + strand, within hypF at 2833.734 kb on + strand, within hypF at 2833.934 kb on - strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.980 kb on - strand, within hypF
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,830,983 + norV b2710 0.34 +1.1 2,830,986 - norV b2710 0.34 -2.1 2,830,986 - norV b2710 0.34 +2.5 2,831,011 - norV b2710 0.36 -1.8 2,831,011 - norV b2710 0.36 -1.4 2,831,018 - norV b2710 0.36 -2.2 2,831,018 - norV b2710 0.36 -0.8 2,831,075 - norV b2710 0.40 +1.7 2,831,075 - norV b2710 0.40 +2.2 2,831,092 - norV b2710 0.41 -2.2 2,831,109 + norV b2710 0.42 -0.4 2,831,134 + norV b2710 0.44 +0.9 2,831,273 + norV b2710 0.54 +1.1 2,831,273 + norV b2710 0.54 -1.3 2,831,314 + norV b2710 0.57 +0.9 2,831,314 + norV b2710 0.57 +1.5 2,831,629 + norV b2710 0.79 -0.4 2,831,646 - norV b2710 0.80 +0.5 2,831,646 - norV b2710 0.80 -0.7 2,831,651 + norV b2710 0.80 +0.2 2,831,706 - norV b2710 0.84 +1.8 2,831,732 - norV b2710 0.86 +0.8 2,831,748 + norV b2710 0.87 +1.5 2,831,748 + norV b2710 0.87 -0.3 2,831,829 + -1.2 2,831,934 - +0.5 2,831,957 - -1.0 2,832,048 + norW b2711 0.10 +0.9 2,832,064 - norW b2711 0.11 -0.5 2,832,150 - norW b2711 0.19 -0.6 2,832,281 + norW b2711 0.31 +1.8 2,832,281 + norW b2711 0.31 +1.9 2,832,364 + norW b2711 0.38 -0.2 2,832,384 - norW b2711 0.40 -1.0 2,832,384 - norW b2711 0.40 +0.4 2,832,526 + norW b2711 0.52 -0.4 2,832,896 - norW b2711 0.85 +2.2 2,832,956 + +2.0 2,833,005 + +3.0 2,833,005 + -0.6 2,833,046 + -1.0 2,833,054 - -0.2 2,833,119 + +1.2 2,833,119 + -0.6 2,833,350 + -0.2 2,833,350 + +2.3 2,833,353 - -1.2 2,833,371 + -2.4 2,833,399 - +0.4 2,833,399 - -1.4 2,833,408 + +3.2 2,833,423 + hypF b2712 0.10 -1.8 2,833,423 + hypF b2712 0.10 +0.2 2,833,581 + hypF b2712 0.17 +1.4 2,833,699 + hypF b2712 0.22 +0.1 2,833,734 + hypF b2712 0.24 -1.8 2,833,934 - hypF b2712 0.33 -2.2 2,833,962 + hypF b2712 0.34 +1.4 2,833,962 + hypF b2712 0.34 -0.1 2,833,980 - hypF b2712 0.35 -1.0
Or see this region's nucleotide sequence