Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acpS and pdxJ overlap by 1 nucleotides pdxJ and recO are separated by 11 nucleotides recO and era are separated by 11 nucleotides era and rnc overlap by 4 nucleotides
b2563: acpS - 4'-phosphopantetheinyl transferase (NCBI), at 2,698,640 to 2,699,020
acpS
b2564: pdxJ - pyridoxal phosphate biosynthetic protein (NCBI), at 2,699,020 to 2,699,751
pdxJ
b2565: recO - DNA repair protein RecO (NCBI), at 2,699,763 to 2,700,491
recO
b2566: era - GTP-binding protein Era (NCBI), at 2,700,503 to 2,701,408
era
b2567: rnc - ribonuclease III (NCBI), at 2,701,405 to 2,702,085
rnc
Position (kb)
2699
2700
2701 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2699.085 kb on - strand at 2699.173 kb on - strand, within pdxJ at 2699.178 kb on - strand, within pdxJ at 2699.290 kb on - strand, within pdxJ at 2699.465 kb on + strand, within pdxJ at 2699.465 kb on + strand, within pdxJ at 2699.504 kb on - strand, within pdxJ at 2699.519 kb on + strand, within pdxJ at 2699.536 kb on + strand, within pdxJ at 2699.536 kb on + strand, within pdxJ at 2699.713 kb on + strand at 2699.774 kb on - strand at 2699.774 kb on - strand at 2699.823 kb on + strand at 2699.924 kb on + strand, within recO at 2700.066 kb on - strand, within recO at 2700.126 kb on + strand, within recO at 2700.128 kb on + strand, within recO at 2700.219 kb on + strand, within recO at 2700.280 kb on - strand, within recO at 2700.280 kb on - strand, within recO at 2700.326 kb on - strand, within recO at 2700.359 kb on - strand, within recO at 2700.467 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,699,085 - -0.5 2,699,173 - pdxJ b2564 0.21 +2.1 2,699,178 - pdxJ b2564 0.22 +1.3 2,699,290 - pdxJ b2564 0.37 -2.8 2,699,465 + pdxJ b2564 0.61 +0.3 2,699,465 + pdxJ b2564 0.61 +1.0 2,699,504 - pdxJ b2564 0.66 -1.5 2,699,519 + pdxJ b2564 0.68 -1.3 2,699,536 + pdxJ b2564 0.70 +0.7 2,699,536 + pdxJ b2564 0.70 -1.5 2,699,713 + +0.7 2,699,774 - -2.8 2,699,774 - -1.0 2,699,823 + -4.0 2,699,924 + recO b2565 0.22 -3.6 2,700,066 - recO b2565 0.42 -3.4 2,700,126 + recO b2565 0.50 -0.9 2,700,128 + recO b2565 0.50 +0.3 2,700,219 + recO b2565 0.63 -2.2 2,700,280 - recO b2565 0.71 -2.0 2,700,280 - recO b2565 0.71 -0.7 2,700,326 - recO b2565 0.77 -3.1 2,700,359 - recO b2565 0.82 -1.0 2,700,467 - -1.7
Or see this region's nucleotide sequence