Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yffQ and yffR are separated by 10 nucleotides yffR and yffS are separated by 150 nucleotides yffS and eutA are separated by 148 nucleotides
b2448: yffQ - CPZ-55 prophage; predicted protein (RefSeq), at 2,561,614 to 2,561,991
yffQ
b2449: yffR - CPZ-55 prophage; predicted protein (NCBI), at 2,562,002 to 2,562,394
yffR
b2450: yffS - CPZ-55 prophage; predicted protein (RefSeq), at 2,562,545 to 2,563,354
yffS
b2451: eutA - reactivating factor for ethanolamine ammonia lyase (NCBI), at 2,563,503 to 2,564,906
eutA
Position (kb)
2562
2563
2564 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2561.561 kb on + strand at 2561.561 kb on + strand at 2561.607 kb on - strand at 2561.652 kb on - strand, within yffQ at 2561.668 kb on - strand, within yffQ at 2561.668 kb on - strand, within yffQ at 2561.805 kb on + strand, within yffQ at 2561.872 kb on - strand, within yffQ at 2561.872 kb on - strand, within yffQ at 2561.872 kb on - strand, within yffQ at 2561.964 kb on + strand at 2561.964 kb on + strand at 2562.073 kb on + strand, within yffR at 2562.073 kb on + strand, within yffR at 2562.092 kb on + strand, within yffR at 2562.092 kb on + strand, within yffR at 2562.114 kb on + strand, within yffR at 2562.122 kb on - strand, within yffR at 2562.140 kb on - strand, within yffR at 2562.169 kb on - strand, within yffR at 2562.407 kb on - strand at 2562.416 kb on + strand at 2562.607 kb on - strand at 2562.611 kb on + strand at 2562.611 kb on + strand at 2562.693 kb on - strand, within yffS at 2562.777 kb on + strand, within yffS at 2562.777 kb on + strand, within yffS at 2563.370 kb on - strand at 2563.429 kb on - strand at 2563.754 kb on - strand, within eutA at 2563.754 kb on - strand, within eutA at 2563.862 kb on - strand, within eutA at 2563.862 kb on - strand, within eutA at 2563.913 kb on - strand, within eutA at 2563.949 kb on - strand, within eutA at 2564.001 kb on + strand, within eutA at 2564.037 kb on - strand, within eutA at 2564.285 kb on + strand, within eutA
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,561,561 + -0.7 2,561,561 + +0.8 2,561,607 - +1.1 2,561,652 - yffQ b2448 0.10 +0.8 2,561,668 - yffQ b2448 0.14 +1.4 2,561,668 - yffQ b2448 0.14 -0.3 2,561,805 + yffQ b2448 0.51 -1.9 2,561,872 - yffQ b2448 0.68 +3.0 2,561,872 - yffQ b2448 0.68 +0.7 2,561,872 - yffQ b2448 0.68 +0.3 2,561,964 + +0.3 2,561,964 + +1.5 2,562,073 + yffR b2449 0.18 +3.0 2,562,073 + yffR b2449 0.18 -1.1 2,562,092 + yffR b2449 0.23 -0.7 2,562,092 + yffR b2449 0.23 -0.2 2,562,114 + yffR b2449 0.28 -1.9 2,562,122 - yffR b2449 0.31 -0.5 2,562,140 - yffR b2449 0.35 +1.8 2,562,169 - yffR b2449 0.42 -0.3 2,562,407 - +1.1 2,562,416 + +0.0 2,562,607 - -1.1 2,562,611 + +0.3 2,562,611 + -2.8 2,562,693 - yffS b2450 0.18 -2.2 2,562,777 + yffS b2450 0.29 -1.5 2,562,777 + yffS b2450 0.29 -0.3 2,563,370 - +1.3 2,563,429 - +0.2 2,563,754 - eutA b2451 0.18 +1.3 2,563,754 - eutA b2451 0.18 -1.1 2,563,862 - eutA b2451 0.26 +3.1 2,563,862 - eutA b2451 0.26 -0.1 2,563,913 - eutA b2451 0.29 -0.1 2,563,949 - eutA b2451 0.32 +2.1 2,564,001 + eutA b2451 0.35 +0.2 2,564,037 - eutA b2451 0.38 +0.8 2,564,285 + eutA b2451 0.56 -0.1
Or see this region's nucleotide sequence