Experiment: Cisplatin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeU and murP are separated by 3 nucleotides murP and b2430 are separated by 4 nucleotides
b2428: yfeU - N-acetylmuramic acid-6-phosphate etherase (NCBI), at 2,543,795 to 2,544,691
yfeU
b2429: murP - N-acetylmuramic acid phosphotransfer permease (NCBI), at 2,544,695 to 2,546,119
murP
b2430: b2430 - putative beta-lactamase (VIMSS), at 2,546,124 to 2,547,428
b2430
Position (kb)
2544
2545
2546
2547 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2543.880 kb on - strand at 2543.935 kb on - strand, within yfeU at 2544.000 kb on + strand, within yfeU at 2544.058 kb on - strand, within yfeU at 2544.130 kb on - strand, within yfeU at 2544.130 kb on - strand, within yfeU at 2544.390 kb on - strand, within yfeU at 2544.425 kb on + strand, within yfeU at 2544.425 kb on + strand, within yfeU at 2544.686 kb on + strand at 2544.693 kb on - strand at 2544.704 kb on - strand at 2544.717 kb on - strand at 2544.727 kb on + strand at 2544.801 kb on + strand at 2544.801 kb on + strand at 2544.811 kb on - strand at 2544.811 kb on - strand at 2544.964 kb on - strand, within murP at 2545.000 kb on - strand, within murP at 2545.234 kb on - strand, within murP at 2545.234 kb on - strand, within murP at 2545.234 kb on - strand, within murP at 2545.236 kb on - strand, within murP at 2545.306 kb on - strand, within murP at 2545.347 kb on + strand, within murP at 2545.445 kb on - strand, within murP at 2545.471 kb on - strand, within murP at 2545.471 kb on - strand, within murP at 2545.494 kb on - strand, within murP at 2545.494 kb on - strand, within murP at 2545.559 kb on + strand, within murP at 2545.559 kb on + strand, within murP at 2545.620 kb on + strand, within murP at 2545.637 kb on + strand, within murP at 2545.761 kb on - strand, within murP at 2545.768 kb on + strand, within murP at 2545.768 kb on + strand, within murP at 2545.786 kb on - strand, within murP at 2545.886 kb on + strand, within murP at 2546.146 kb on - strand at 2546.233 kb on - strand at 2546.239 kb on + strand at 2546.412 kb on + strand, within b2430 at 2546.420 kb on - strand, within b2430 at 2546.420 kb on - strand, within b2430 at 2546.423 kb on + strand, within b2430 at 2546.489 kb on - strand, within b2430 at 2546.489 kb on - strand, within b2430 at 2546.663 kb on - strand, within b2430 at 2546.674 kb on + strand, within b2430 at 2546.758 kb on + strand, within b2430 at 2546.844 kb on + strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2546.969 kb on - strand, within b2430 at 2547.030 kb on + strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.062 kb on - strand, within b2430 at 2547.071 kb on - strand, within b2430 at 2547.087 kb on - strand, within b2430
Per-strain Table
Position Strand Gene LocusTag Fraction Cisplatin 0.1 mg/ml remove 2,543,880 - -0.6 2,543,935 - yfeU b2428 0.16 -0.8 2,544,000 + yfeU b2428 0.23 -1.3 2,544,058 - yfeU b2428 0.29 -1.3 2,544,130 - yfeU b2428 0.37 +1.6 2,544,130 - yfeU b2428 0.37 +2.4 2,544,390 - yfeU b2428 0.66 +0.0 2,544,425 + yfeU b2428 0.70 -1.6 2,544,425 + yfeU b2428 0.70 -0.5 2,544,686 + +2.6 2,544,693 - -1.6 2,544,704 - +0.8 2,544,717 - -1.2 2,544,727 + -0.8 2,544,801 + +0.2 2,544,801 + +1.2 2,544,811 - +0.2 2,544,811 - +0.0 2,544,964 - murP b2429 0.19 +1.0 2,545,000 - murP b2429 0.21 +0.7 2,545,234 - murP b2429 0.38 +0.8 2,545,234 - murP b2429 0.38 -1.6 2,545,234 - murP b2429 0.38 -0.1 2,545,236 - murP b2429 0.38 -0.5 2,545,306 - murP b2429 0.43 -0.9 2,545,347 + murP b2429 0.46 -0.3 2,545,445 - murP b2429 0.53 +0.6 2,545,471 - murP b2429 0.54 -0.3 2,545,471 - murP b2429 0.54 +2.2 2,545,494 - murP b2429 0.56 -0.3 2,545,494 - murP b2429 0.56 +0.2 2,545,559 + murP b2429 0.61 -1.9 2,545,559 + murP b2429 0.61 -0.2 2,545,620 + murP b2429 0.65 -0.1 2,545,637 + murP b2429 0.66 -1.0 2,545,761 - murP b2429 0.75 -0.4 2,545,768 + murP b2429 0.75 -1.6 2,545,768 + murP b2429 0.75 -2.2 2,545,786 - murP b2429 0.77 -0.3 2,545,886 + murP b2429 0.84 -1.6 2,546,146 - -0.8 2,546,233 - -1.5 2,546,239 + -0.3 2,546,412 + b2430 b2430 0.22 -1.5 2,546,420 - b2430 b2430 0.23 -1.6 2,546,420 - b2430 b2430 0.23 -1.3 2,546,423 + b2430 b2430 0.23 -0.3 2,546,489 - b2430 b2430 0.28 -0.3 2,546,489 - b2430 b2430 0.28 +2.1 2,546,663 - b2430 b2430 0.41 +1.8 2,546,674 + b2430 b2430 0.42 -0.2 2,546,758 + b2430 b2430 0.49 +1.7 2,546,844 + b2430 b2430 0.55 -0.9 2,546,969 - b2430 b2430 0.65 -0.3 2,546,969 - b2430 b2430 0.65 +0.8 2,547,030 + b2430 b2430 0.69 +1.1 2,547,062 - b2430 b2430 0.72 +0.9 2,547,062 - b2430 b2430 0.72 -1.1 2,547,071 - b2430 b2430 0.73 +0.1 2,547,087 - b2430 b2430 0.74 -0.5
Or see this region's nucleotide sequence